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Preparing method for preservative agent for chicken newcastle disease virus

A technology of chicken Newcastle disease virus and preservative, which is applied in the field of poultry biological products, can solve the problem of insufficient stability of virus hemagglutination titer, and achieve the effect of favorable development and utilization and great application potential

Inactive Publication Date: 2003-01-22
WUHAN INST OF VIROLOGY CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But the problem is: With the development and utilization of chicken Newcastle disease virus resources, it is necessary to store the virus solution in a refrigerator at 4-8°C for one month or more, but the virus solution should be stored at 4-8°C for 3-4 hours. Days later, the virus hemagglutination titer will drop rapidly, and the chicken Newcastle disease virus hemagglutination titer is not stable enough

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] (a) Get 9-day-old chicken embryos, sterilize the egg shells with iodine and 75% alcohol in a sterile room, punch a small hole in the air cell, and inoculate the NDV-F strain (NDV-F) with a sterile syringe. The F virus strain is preserved by freeze-drying, and the virus hemagglutination titer is 640; 0.2ml is diluted 20 times with sterile normal saline in advance, sealed with nail polish, and placed in a 36°C incubator for incubation for 4-5 days. After 5 days, take it out and put it in a refrigerator at 4°C overnight, and take out the allantoic fluid under aseptic conditions, and the allantoic fluid is the proliferated NDV-F virus.

[0012] (b) The proliferated allantoic fluid is sterilized by filtration with a 0.45μ microporous membrane, and the broth culture solution is used to detect whether there is bacterial contamination, and the sterility test should be negative. The hemagglutination titer was determined to be 640.

[0013] (c) Add 1 g of deoxyribonuclease (DNas...

Embodiment 2

[0018] (a) Get 10-day-old chicken embryos, sterilize the eggshells with iodine and 75% alcohol in a sterile room, punch a small hole in the air cell, and inoculate the NDV-F strain (NDV-F) with a sterile syringe. The F virus strain is preserved by freeze-drying, and the virus hemagglutination titer is 640; 0.2ml is diluted 20 times with sterile normal saline in advance, sealed with nail polish, and placed in a 36°C incubator for incubation for 4-5 days. After 5 days, take it out and put it in a refrigerator at 4°C overnight, and take out the allantoic fluid under aseptic conditions, and the allantoic fluid is the proliferated NDV-F virus.

[0019] (b) The proliferated allantoic fluid is sterilized by filtration with a 0.45μ microporous membrane, and the broth culture solution is used to detect whether there is bacterial contamination, and the sterility test should be negative. The hemagglutination titer was determined to be 640.

[0020] (c) Add 1 g of deoxyribonuclease (DNas...

Embodiment 3

[0025] (a) Get 9-day-old chicken embryos, sterilize the egg shells with iodine and 75% alcohol in a sterile room, punch a small hole in the air cell, and inoculate the NDV-F strain (NDV-F) with a sterile syringe. The F virus strain is preserved by freeze-drying, and the virus hemagglutination titer is 640; 0.2ml is diluted 20 times with sterile normal saline in advance, sealed with nail polish, and placed in a 36°C incubator for incubation for 4-5 days. After 5 days, take it out and put it in a refrigerator at 4°C overnight, and take out the allantoic fluid under aseptic conditions, and the allantoic fluid is the proliferated NDV-F virus.

[0026] (b) The proliferated allantoic fluid is sterilized by filtration with a 0.45μ microporous membrane, and the broth culture solution is used to detect whether there is bacterial contamination, and the sterility test should be negative. The hemagglutination titer was determined to be 640.

[0027] (c) Add 1 g of deoxyribonuclease (DNas...

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PUM

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Abstract

The present invention discloses a preparation process of preservative agent of chicken Newcastle disease virus. The preparation process includes weighing deoxyribonuclease, dissolving with distilled water, concentration and filtering; weighing ribonuclease, dissolving with distilled water, concentration and filtering; adding the concentrated deoxyribonuclease liquid and the concentrated ribonuclease liquid into the virus solution to result in the concentration of 50-250 microngram each ml and 25-125 microngram each ml separately; and shaking to homogenize and preservation. The said process can prolong the preservation period of virus liquid and maintain the virus potency stably.

Description

technical field [0001] The invention relates to poultry biological products, and more specifically relates to a preparation method of chicken Newcastle disease virus preservation agent. Background technique [0002] Chicken Newcastle disease virus (Newcastle disease virus NDV) has been used in the production of chicken plague vaccine, and plays a decisive role in the prevention and treatment of chicken viral diseases. In the 1980s, as an inducer, it was widely used in human leukocyte interferon and antitumor drug cicrimycin (BC 369 ) production. Changjiang Daily reported in February 2001 that the general surgery department of Wuhan Union Medical College Hospital used chicken Newcastle disease virus to change 20% of the surface antigens of captured cancer cells to prepare tumor vaccines. At present, Newcastle disease virus (NDV) at home and abroad is generally preserved by freeze-drying method and -30 ℃ low-temperature refrigerator method, and the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N7/01
Inventor 陈绳亮李天宪严哲熊克娟范兆军张忠王瑶
Owner WUHAN INST OF VIROLOGY CHINESE ACADEMY OF SCI
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