Preparing method for preservative agent for chicken newcastle disease virus

Abstract

The present invention discloses a preparation process of preservative agent of chicken Newcastle disease virus. The preparation process includes weighing deoxyribonuclease, dissolving with distilled water, concentration and filtering; weighing ribonuclease, dissolving with distilled water, concentration and filtering; adding the concentrated deoxyribonuclease liquid and the concentrated ribonuclease liquid into the virus solution to result in the concentration of 50-250 microngram each ml and 25-125 microngram each ml separately; and shaking to homogenize and preservation. The said process can prolong the preservation period of virus liquid and maintain the virus potency stably.

Description

technical field

[0001] The invention relates to poultry biological products, and more specifically relates to a preparation method of chicken Newcastle disease virus preservation agent. Background technique

[0002] Chicken Newcastle disease virus (Newcastle disease virus NDV) has been used in the production of chicken plague vaccine, and plays a decisive role in the prevention and treatment of chicken viral diseases. In the 1980s, as an inducer, it was widely used in human leukocyte interferon and antitumor drug cicrimycin (BC 369 ) production. Changjiang Daily reported in February 2001 that the general surgery department of Wuhan Union Medical College Hospital used chicken Newcastle disease virus to change 20% of the surface antigens of captured cancer cells to prepare tumor vaccines. At present, Newcastle disease virus (NDV) at home and abroad is generally preserved by freeze-drying method and -30 ℃ low-temperature refrigerator method, and the ...