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Breeding method for heterotrophic chlorella protothecoides

A technology of heterotrophic chlorella and culture medium, applied in the direction of microorganism-based methods, biochemical equipment and methods, single-cell algae, etc., can solve the problem of low biological content of algae cells, long cultivation time, and complicated chlorella methods and other problems, to achieve the effect of high biological content of algae cells, short cultivation time and simple cultivation process

Inactive Publication Date: 2014-06-25
QINGDAO ZHONGREN PHARMA
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Problems solved by technology

[0006] In order to overcome the above-mentioned defects existing in the prior art field, the object of the present invention is to provide a method for breeding heterotrophic chlorella, which solves the problem that the method for cultivating chlorella in the prior art is relatively complicated, the cultivation time is long, and the algal cell The problem of low biological content

Method used

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Embodiment

[0017] The breeding method of the heterotrophic chlorella of the present embodiment comprises the following steps:

[0018] (1) Adjust the pH of the culture medium to 7-8, sterilize at 120°C, 0.10-0.18Mp for 30 minutes;

[0019] (2) Take 100ml of the sterilized culture medium, add 1-2g of agar to dissolve it, sterilize it, pour it into a sterilized petri dish, cool and solidify, and make a screening plate;

[0020] (3) Take a water sample containing green water from a natural water body, centrifuge it in a centrifuge, pour off the supernatant, suspend the cells sinking at the bottom of the centrifuge tube with sterile culture medium, and then centrifuge to remove the supernatant After 3-5 repetitions, finally, add the culture medium to the centrifuge tube to suspend the algae cells along the bottom, draw 2ml of the algae cell suspension, apply it evenly on the surface of the screening plate, and place it under the light condition of 20-35°C After 5-10 days of culture, green m...

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Abstract

The invention discloses a breeding method for heterotrophic chlorella protothecoides. The breeding method comprises the steps of adjusting a pH value of a culture solution to 7-8; carrying out high temperature sterilization for 30 min at a temperature of 120 DEG C and a pressure of 0.10-0.18 Mp; then preparing a screening flat plate; then sampling and screening chlorella protothecoides; inoculating chlorella protothecoides on a sterile heterotrophic culture solution in a triangular flask; and carrying out culture for 5 days in dark with an oscillating rotation speed of 200 r / min and a temperature of 25 DEG C. A culture process is simple; a culture time is short; and biomass content of the chlorella protothecoides cells is relatively high.

Description

[0001] technical field [0002] The invention belongs to the technical field of microalgae cultivation, and in particular relates to a breeding method for heterotrophic chlorella. [0003] Background technique [0004] Chlorella cells are rich in chlorophyll, which belongs to single-celled green algae and is a eukaryote. Chlorella is rich in CGF (Chlorella Growth Factor), which can quickly restore the damage caused by the body, and can be used as a food flavor improver, widely used in food and fermentation fields. Chlorella is produced in fresh or salt water. Freshwater species often grow in relatively fertile water bodies, and can sometimes be found on wet soil, rocks, and tree trunks. There are not many individuals in the water body under natural conditions, but it can grow and reproduce in large quantities under artificial cultivation conditions, with a high yield. The protein content is about 50% of the dry weight, and it is also rich in other components. The existin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12R1/89
Inventor 张述智夏伟朱绍辉张浩王晓丽徐权汗李之详许团辉
Owner QINGDAO ZHONGREN PHARMA
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