Exfoliated cell chip

Abstract

The present invention provides a technology and a method for preparing a high-throughput exfoliated cell chip. According to the present invention, a cervix brush is adopted to collect cervix exfoliated cells of 26 cervical lesions patients and are immediately placed into a cell preservation solution, centrifugation is performed, the supernatant is removed, a cell immobilization solution is added to immobilize cell precipitate, dehydration transparency and dipping in wax are performed, a tissue chip instrument is adopted to prepare an exfoliated cell paraffin chip and an exfoliated cell paraffin block array, and paraffin slicing is performed to prepare the exfoliated cell chip; and the defect of the conventional cytology detection is overcome, and the prepared exfoliated cell chip can be used for pathological diagnosis, IHC, in situ hybridization and other molecular medicine researches.

Description

technical field [0001] The invention belongs to the field of biochips, and relates to a biochip for preparing microarray shedding chips and a preparation method. Background technique [0002] Biochip refers to a high-throughput microarray of high-density tissues, cells, proteins, nucleic acids, sugars, and other biological components coated on solid supports such as silicon wafers and nylon membranes. Utilizing high-throughput biochips and specific molecular hybridization and staining techniques to detect the tested samples, a large amount of molecular detection information can be obtained accurately and quickly. At present, it has been widely used in the fields of life science research, molecular diagnosis of clinical medicine, and screening and verification of biological antibody-targeted drugs. [0003] Exfoliated cytology examination is one of the important means for early diagnosis of cancer. Its basic operating procedure is: centrifuge to collect exfoliated cells, slo...

AI Technical Summary

Problems solved by technology

Its disadvantages are: 1. Cell overlap is very easy to appear on the prepared cell smear, making it difficult to identify tumor cells; 2. Tumor cells may be covered by other fragmented tissues or tissue clumps, resulting in false negative test results; 3. . The exfoliated tumor cell clumps cannot be effectively dispersed, which leads to difficulties in pathological diagnosis and typing; 4. During the staining process, tumor cells that are not effectively absorbed by the glass slide may be eluted, resulting in false negative results; 5. Due to the use of cytological staining for cells, it is difficult to carry out clear pathological staging and typing of tumor cells

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