Exfoliated cell chip

A technology of exfoliated cells and chips, which is applied in the field of biochips and preparations for the preparation of microarray exfoliated chips, which can solve the problems of false negative test results, difficult pathological diagnosis and typing, false negatives, etc.

Inactive Publication Date: 2014-07-02
泰州医药城博奥邦科生物科技有限公司
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Problems solved by technology

Its disadvantages are: 1. Cell overlap is very easy to appear on the prepared cell smear, making it difficult to identify tumor cells; 2. Tumor cells may be covered by other fragmented tissues or tissue clumps, resulting in false negative test results; 3. . The exfoliated tumor cell clumps cannot be effectively dispersed, which lea...

Method used

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Examples

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Embodiment Construction

[0009] Example: On the basis of fully informed consent, cervical exfoliated cells of 42 patients with cervical lesions were collected to prepare exfoliated cell chips.

[0010] 1.1 Collection of exfoliated cells: The cervical exfoliated cells of 42 patients with cervical lesions were collected by using a cervical brush, and immediately put into the cell preservation solution to fully elute the cells on the cervical brush. Centrifuge, remove the supernatant, and collect the exfoliated cell pellet.

[0011] 1.2 Fixation of exfoliated cells: Add 1ml of cell fixation solution to the exfoliated cell pellet, mix thoroughly, and fix at room temperature for 1 hour.

[0012] 1.3 Dehydration and embedding: the exfoliated cells were collected by centrifugation, and the exfoliated cells were washed twice with neutral PBS buffer. After alcohol gradient dehydration, the degreased and transparent cells were collected by centrifugation. Add exfoliated cells to 50ul preheated melted paraffin...

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Abstract

The present invention provides a technology and a method for preparing a high-throughput exfoliated cell chip. According to the present invention, a cervix brush is adopted to collect cervix exfoliated cells of 26 cervical lesions patients and are immediately placed into a cell preservation solution, centrifugation is performed, the supernatant is removed, a cell immobilization solution is added to immobilize cell precipitate, dehydration transparency and dipping in wax are performed, a tissue chip instrument is adopted to prepare an exfoliated cell paraffin chip and an exfoliated cell paraffin block array, and paraffin slicing is performed to prepare the exfoliated cell chip; and the defect of the conventional cytology detection is overcome, and the prepared exfoliated cell chip can be used for pathological diagnosis, IHC, in situ hybridization and other molecular medicine researches.

Description

technical field [0001] The invention belongs to the field of biochips, and relates to a biochip for preparing microarray shedding chips and a preparation method. Background technique [0002] Biochip refers to a high-throughput microarray of high-density tissues, cells, proteins, nucleic acids, sugars, and other biological components coated on solid supports such as silicon wafers and nylon membranes. Utilizing high-throughput biochips and specific molecular hybridization and staining techniques to detect the tested samples, a large amount of molecular detection information can be obtained accurately and quickly. At present, it has been widely used in the fields of life science research, molecular diagnosis of clinical medicine, and screening and verification of biological antibody-targeted drugs. [0003] Exfoliated cytology examination is one of the important means for early diagnosis of cancer. Its basic operating procedure is: centrifuge to collect exfoliated cells, slo...

Claims

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Application Information

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IPC IPC(8): G01N1/28
Inventor 郜恒骏张可浩张新朱华玉张宇马小慧
Owner 泰州医药城博奥邦科生物科技有限公司
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