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Establishment method of fingerprint spectrum of fragrant plantain lily flower in mongolian medicine, and standard fingerprint spectrum of fragrant plantain lily flower in mongolian medicine

A standard fingerprint, fingerprint technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of reflecting the chemical connotation and quality of hosta flower medicinal materials, and achieve the analysis method is simple and easy, good reproducibility, and precision. high effect

Inactive Publication Date: 2014-07-16
INNER MONGOLIA MEDICAL UNIV
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  • Abstract
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Problems solved by technology

Neither the statutory current standards nor the existing standard research can fully and comprehensively reflect the chemical connotation and quality of hosta medicinal materials from an overall perspective

Method used

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  • Establishment method of fingerprint spectrum of fragrant plantain lily flower in mongolian medicine, and standard fingerprint spectrum of fragrant plantain lily flower in mongolian medicine
  • Establishment method of fingerprint spectrum of fragrant plantain lily flower in mongolian medicine, and standard fingerprint spectrum of fragrant plantain lily flower in mongolian medicine
  • Establishment method of fingerprint spectrum of fragrant plantain lily flower in mongolian medicine, and standard fingerprint spectrum of fragrant plantain lily flower in mongolian medicine

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Experimental program
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Effect test

Embodiment Construction

[0059] The technical solution of the present invention will be described in further detail below in conjunction with the accompanying drawings and specific embodiments.

[0060] 1 Instruments and materials

[0061] 1.1 Instruments and reagents

[0062] Ultra-high performance liquid chromatography Thermo UltiMate3000 series: binary high-pressure gradient pump, autosampler, column thermostat, DAD detector; AB135-S electronic balance (METTLER TOLEDO); KH3200E ultrasonic cleaning machine (Kunshan Hechuang Ultrasonic Cleaning Co., Ltd. Company); Methanol is chromatographically pure (Fisher, USA); Ethanol is analytically pure (Tianjin Weichen Chemical Reagent Science and Trade Co., Ltd.); other reagents are analytically pure.

[0063] 1.2 Materials

[0064] Chromatographic column used in the experiment: (a) Alltima5μC 18 (250mm×4.6nm); (b) YMC-Pack ODS-A (250mm×4.6mm D5μm, 12nm); (c) COSMOSIL C 18 (250mm×4.6mm ID)

[0065] 1.3 Reagents: The reference substances were all isolate...

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Abstract

The invention discloses an establishment method of a fingerprint spectrum of a fragrant plantain lily flower in mongolian medicine, and a standard fingerprint spectrum of the fragrant plantain lily flower in the mongolian medicine. The method comprises the following steps: precisely weighing 0.5g of fragrant plantain lily flower medicinal material powder; arranging the medicinal material powder in a 100mL conical flask with a cover; precisely adding 60mL of 60% ethyl alcohol; performing supersonic extraction for 45min; filtering to obtain filter residue; cleaning the filter residue three times with the 60% ethyl alcohol, wherein 10mL of the ethyl alcohol is consumed each time; performing decompression recycling on solvents until the solvents are dry; making to constant volume of the dry recovered solvents into a 25mL measuring flask by using 10% methyl alcohol; diluting to the scale; shaking up uniformly; filtering by a micro-pore filter film to obtain subsequent filtrate for later use; and performing chromatographic step, wherein the chromatographic conditions are as follows: a chromatographic column is Alltima5muC18, a flowing phase is methyl alcohol-water, the elution gradient is 5%B0min-10%B25min-27%B58min-40%B61min70%B80min-100%B90min, the detection wave length is 265nm; the column temperature is 30 DEG C, the flowing speed is 1mL / min, and the sampling size is 20muL. A high performance liquid chromatography is adopted for performing fingerprint spectrum researches on the fragrant plantain lily flower in the mongolian medicine, and the establishment method is used for evaluating the validity, the excellent performance and the stability of the quality of the fragrant plantain lily flower medicinal material.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a method for establishing a fingerprint of Mongolian medicine hosta flower and a standard fingerprint thereof. Background technique [0002] In recent years, the international botanical drug market demand has been increasing, and our country's botanical drug resources are facing a serious shortage of medicinal raw materials. Due to the influence of a series of processes such as the base source, the environment and climate of the place of origin, the harvest time, and the processing method, the ingredients contained in the raw materials of Chinese and Mongolian medicines often vary greatly, and the authenticity, uniformity, and stability of the quality are difficult to guarantee. At the same time, under the lure of huge profits, many drug companies disregarded the safety of consumers and passed off fakes as real ones. The quality problem of Chinese and Mongolian medicine has become a t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
Inventor 薛培风魏慧解红霞李春燕王娜娜李晓娟王丽王静张金花赵子龙高建萍
Owner INNER MONGOLIA MEDICAL UNIV
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