Drug-resistant cell strain of human bile duct cancer and application of drug-resistant cell strain

A technology of drug-resistant cells and cholangiocarcinoma, applied in the field of tumor biology, can solve the problems affecting the drug-resistant cell line's drug resistance mechanism and drug resistance degree, poor chemotherapy effect, etc., and achieve the effect of high scientific research and production application value.

Active Publication Date: 2014-07-23
山东国九堂制药集团股份有限公司
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Problems solved by technology

The combined use of 5-FU and cisplatin is considered to be one of the standard treatment methods, and the tumor response rate is reported to be 20% to 40%, while the chemotherapy effect of 5-FU alone is poor
The comparative study of the above two methods suggests that different induction methods will affect the drug resistance mechanism and degree of drug resistance of the obtained drug-resistant cell lines

Method used

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  • Drug-resistant cell strain of human bile duct cancer and application of drug-resistant cell strain
  • Drug-resistant cell strain of human bile duct cancer and application of drug-resistant cell strain
  • Drug-resistant cell strain of human bile duct cancer and application of drug-resistant cell strain

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Embodiment

[0025] 1. Cell culture method.

[0026] The culture method of the human cholangiocarcinoma drug-resistant cell line of the present embodiment, the specific steps are as follows:

[0027] A. Add the cells (human cholangiocarcinoma cells RBE provided by the Cell Room of the Chinese Academy of Sciences) into the cell culture medium (DMEM medium containing 10% calf serum), and place at 37°C, 5% CO 2 , cultured in an incubator with saturated humidity, digested and passaged with 0.25% trypsin.

[0028] B. When the cells are cultured until the cell density reaches 80% (or cultured to the logarithmic growth phase), replace fresh cell culture medium, and add 5-FU to the cell culture medium, so that the concentration of 5-FU in the cell culture medium The effect concentration is 25 μg / ml, and then placed at 37°C, 5% CO 2 , in an incubator with saturated humidity, discard the 5-FU cell culture medium after 24 hours, and wash it twice with PBS solution.

[0029] C. Repeat the method of...

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Abstract

The invention relates to a drug-resistant cell strain of human bile duct cancer. The drug-resistant cell strain has a collection number of CCTCC NO:C 201441. A human bile duct cancer RBE cell strain is selected as a parent cell and is added into 5-FU for culture, the action concentration is gradually increased according to a concentration sequence of 25, 50, 75 and 100mu g / ml, and the drug-resistant cell strain of human bile duct cancer is established. The drug-resistant cell strain RBE / 5-FU of the human bile duct cancer can provide a drug-resistant tumor cell model for researching a drug-resistant tumor mechanism, analyzing the sensitivity of chemotherapeutics, screening and evaluating the chemotherapeutics, developing drug-resistant tumor reversing drugs and researching more effective tumor treatment methods.

Description

technical field [0001] The invention relates to a drug-resistant cell line, which belongs to the field of tumor biology. Background technique [0002] Cholangiocarcinoma refers to malignant tumors originating from the extrahepatic bile duct, including the hilar area to the lower end. Cholangiocarcinoma accounted for 2.88% to 4.65% of all malignant tumor deaths. The incidence of cholangiocarcinoma is increasing year by year, and the incidence of cholangiocarcinoma has even doubled in the past ten years in some high-incidence areas. The age of onset is mostly 50-70 years old, but it can also be seen in young people. Curative resection is still the only option for patients with cholangiocarcinoma. However, due to the late onset of clinical symptoms and high degree of malignancy of cholangiocarcinoma, the long-term postoperative effect is still not optimistic. [0003] At present, the experience of chemotherapy for hilar cholangiocarcinoma is limited, and the therapeutic effe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/09C12Q1/02C12R1/91
Inventor 于观贞徐冬云王杰军徐英杰陈智伟沈涤华王勇赵勇门琼黄春锦李亮李晓勇陈宏
Owner 山东国九堂制药集团股份有限公司
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