Method suitable for high-quality RNA isolation of south Xinjiang-specific fruit trees

Abstract

The invention relates to a method suitable for high-quality RNA isolation of south Xinjiang-specific fruit trees. In the method, a 'borax-CTAB' method and a 'modified CTAB' method are combined, namely borax is added into an extraction buffer solution, and main technical process mainly refers to the process of the 'modified CTAB' method; in combination of own characteristics of the south Xinjiang-specific fruit trees (walnut trees, pear trees, almond trees,, apricot trees,and apple trees), in the method, a set of technology system suitable for the high-quality RNA isolation of the south Xinjiang-specific fruit trees (walnut trees, pear trees, almond trees, apricot trees and apple trees) is redetermined. The method comprises the following specific steps: determining the operating ambient temperature, determining the 65 DEG C hot-bath extraction time, determining the rotation speed and the time of high-speed repeated refrigerated centrifugation extraction, and determining the rotation speed and the time of high-speed refrigerated centrifugation of RNA. Through repeated experimental verification, the method is very stable in repeated effect, very high in reproducibility and higher in operability.

Description

technical field [0001] The invention relates to a method suitable for isolating high-quality RNA of characteristic fruit trees in southern Xinjiang, and belongs to the field of fruit tree biotechnology. Background technique [0002] The existing RNA isolation technology system for plants, as well as the RNA isolation technology system that has been better applied to a variety of fruit trees, have been tested on characteristic fruit trees in southern Xinjiang, and the effect is not obvious, especially unstable. Sex is also not very good, sometimes it can be separated, sometimes there is no result. In response to this shortcoming, the purpose of the present invention is to explore a method for RNA isolation on characteristic fruit trees in southern Xinjiang that is stable and reproducible. Only in this way will it not delay the time to complete scientific research tasks and avoid repeated exploration by scientific researchers. process. Contents of the invention [0003] Th...

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Problems solved by technology

[0002] The existing RNA isolation technology system for plants, as well as the RNA isolation technology system that has been better applied to a variety of fruit trees, have been tested on characteristic fruit trees in southern Xinjiang, and the effect is not obvious, especially unstable. The sex is also not very good, sometimes it can be separated, sometimes there is no result