65results about How to "Reproducible and stable" patented technology

The invention belongs to the technical fields of water pollution treatment and photocatalytic materials, and particularly relates to a visible-light-driven photocatalyst Ag-AgCl-BiOCl and a preparation method and application thereof. The visible-light-driven photocatalyst is a composite photocatalyst which is obtained from AgCl and BiOCl through a coprecipitation method and denoted as Ag-AgCl-BiOCl. The preparation method comprises the steps that ethanediol and water are taken as solvent, the BiOCl-AgCl are subjected to coprecipitation in the solvent, precipitation is irradiated through a xenon lamp, part AgCl is resolved into Ag, and the Ag-AgCl-BiOCl composite photocatalyst is finally formed. The composite photocatalyst effectively increases absorption of the BiOCl within a visible light range, a larger specific surface area and the higher electron-hole separation efficiency are showed, and compared with pure BiOCl and AgCl, better adsorbability and photocatalytic activity are achieved. The visible-light-driven photocatalyst Ag-AgCl-BiOCl can be widely used for dye and pharmaceutical wastewater treatment.

An arc end tooth addendum measuring method comprises the following steps: using the scanning probe of a three-coordinate measuring machine to measure the addendum of an arc end tooth, and acquiring actual contour data points of a standard concave tooth at the reference circle of the end tooth through a cylindrical scanning function; establishing a rectangular coordinate system, measuring a datum plane A, determining a z-axis direction and an oxy plane, measuring a benchmark hole, setting the center of the hole as the origin of coordinates o, and taking any direction as an x-axis direction; and setting end tooth measurement parameters, selecting Edit scanning data in the three-coordinate measuring machine, and setting selected unknown circumferential scanning. By using the arc end tooth addendum measuring method, the detection precision of standard arc end tooth addendum is improved.

The invention relates to a preparation method for super-hydrophobic chitosan oil-absorbing sponge. The preparation method comprises the following steps: (1) preparing chitosan-based sponge with a three-dimensional porous structure by using a vacuum freeze-drying method; (2) preparing modified Fe3O4 particles, and enabling the modified Fe3O4 particles and nano zinc oxide particles to be loaded in the three-dimensional porous structure of the chitosan-based sponge; and (3) soaking the obtained chitosan-based sponge in octadecanethiol-absolute ethyl alcohol solution, and repeatedly compressing and expanding, after curing, to obtain a product. Compared with existing chitosan-based sponge, the product has excellent hydrophobic oil-absorbing properties, and a high repeated utilization ratio.

The invention discloses a method for realizing efficient differentiation of induced pluripotent stem cells towards mature endothelial cells, belonging to the technical field of cell engineering. The method comprises the following steps: carrying out pretreatment culturing on the induced pluripotent stem cells; carrying out induction to form side plate mesoblastic cells; carrying out inducing to form endothelial progenitor cells tending to the endothelial cells; and carrying out inducing to form the mature endothelial cells. According to the method, various cell inducing factors are matched through increase and decrease, meanwhile, the reasonable upgrading is carried out on the inducing method, thus the directional differentiation of the induced pluripotent stem cells towards the endothelial cells can be efficiently induced, under the condition that a small number of induced pluripotent stem cells are adopted and the purifying operation is not needed, a large number of mature endothelial cells with high purity and complete functions are obtained.

The invention discloses a method for preparing a strain sensitivity-conductivity silicone rubber for a precise resistor and relates to a conductivity-strain sensitivity rubber material with low modulus and high repeatability. The preparation method comprises the following steps: the surface of a high-conductivity nanometer ruthenium oxide powder body is subjected to hydrophilization treatment; the surface of the nanometer ruthenium oxide powder body is modified through a silane coupling agent of which a terminal group is an ethylene group to form the conductive ruthenium oxide powder body containing the ethylene group on the surface; the conductive ruthenium oxide powder body is mixed with silicone rubber; the volume percentage of the ruthenium oxide powder body accounts for 17 to 30 percent of the total volume of the mixture; the mixture is added with a vulcanizing agent and a strengthening agent, is mixed, is kept stand for 30 minutes, is put to a mould, is pressurized to a pressure of between 10 and 15 Mpa, is kept at a temperature of between 172 and 178 DEG C for 15 to 30 minutes to obtain the vulcanized silicone rubber composite material; and the conductivity of the vulcanized silicone rubber composite material has the characteristic of repeated change along strain.

The invention provides a quality control method of a pummelo pee medicinal material based on gene level. The quality control method comprises the following steps: (1) extracting genome DNAs of a genuine pummelo pee medicinal material and a medicinal material to be detected; (2) taking the extracted genome DNAs as primers in sequence numbers of 1, 2, 3, 4, 5, 6, 7, 8, 9, 10 and 11 respectively to perform polymerase chain reaction; (3) respectively performing electrophoretic separation on products from the polymerase chain reaction by agarose gel electrophoresis; and (4) comparing electrophoretograms of the sample to be detected and the genuine pummelo pee medicinal material. The method has the advantages of good stability and repeatability, and helps obtain the genetic relation between the medicinal material to be detected and the genuine medicinal material, can help ensure selection of fine varieties based on the gene level and helps ensure the quality of medicinal materials from the source.

The invention discloses a molecular culture medium for culturing small intestine organoids of pigs. The molecular culture medium consists of a WNR-J2 condition culture medium and a basal culture medium, and the volume percentage concentration of the WNR-J2 condition culture medium in the molecular culture medium is 50-60%, and the basic culture medium is composed of Advanced DMEM/F12, an HEPES buffer solution, a B27 serum substitute, gastrin I, N-acetyl-L-cysteine, niacinamide10M, L-glutamine, N2-MAX, ethanolamine and an EGF epidermal growth factor. The molecular culture medium is suitable forsmall intestine crypt separation and culture of suckling piglets, weaned piglets and fattening pigs, long-term passage and maintenance of the small intestine organs of the pigs and intestinal nutrition and disease modeling of the pigs.

The invention relates to the field of a lead-acid storage battery, and discloses a <pi>-type-conjugated oxidation reduction polymer modified lead storage battery and a preparation method therefor. Positive electrode lead paste of the battery comprises 98.5-99.5 parts of lead powder, 0.2-0.8 parts of additive and 0.3-0.7 parts of mixed polymer powder; and the mixed polymer powder is prepared from a <pi>-type-conjugated oxidation reduction polymer, a surface anti-oxidation functional group sulfonated arene polymer and single-wall carbon nanotubes. The active material of the lead storage battery comprises the surface-modified and anti-oxidation functional group conjugated oxidation reduction polymers, so that strong electronic storage capability and rapid electronic reaction speed are achieved; the polymers comprise available oxidation reduction nodes, so that relatively strong oxidation reduction activity is achieved; therefore, the lead storage battery has relatively high energy density, and the discharging time of the battery is obviously prolonged in a high-rate discharging condition of the battery; and meanwhile, the service life of the battery is prolonged.

The invention discloses a memristor based on a metalloporphyrin heterojunction, a preparation method thereof and application. The memristor comprises a bottom electrode, a heterojunction layer, a metal oxide layer and a top electrode arranged in sequence from bottom to top, wherein the heterojunction layer comprises two metalloporphyrin layers for regulating the electrical properties of the memristor; and the metal oxide layer is used for providing ions and vacancies required for the operation of the memristor. The metalloporphyrin heterojunction memristor has a simple preparation process, is stable in properties, is suitable for a flexible device, can be processed in a large area, has changeable and adjustable property indexes and has the voltage-dependent filtering characteristics; and besides, the device yield, the repetition and the property stability are high.

The invention discloses a shift-by-wire switch test device, system and method based on a six-degree-of-freedom mechanical arm. The test device comprises a position information management system for establishing a space coordinate system for determining all working planes of the mechanical arm in a working area of the mechanical arm, a test module for sending a test instruction corresponding to a test case according to the test case selected by a user, a motion control module and a shooting module. The test module is also used for obtaining the gear, where a shift-by-wire switch locates, according to a position map, judging whether the gear obtained when the mechanical arm reaches a preset target position corresponding to the test instruction is consistent with the gear, where the shift-by-wire switch locates, and testing whether the shift-by-wire switch is qualified. The shift-by-wire switch test device, system and method comprise the six-degree-of-freedom mechanical arm and the test system corresponding to the six-degree-of-freedom mechanical arm; motion redundancy thereof can meet test requirements of a new shift-by-wire switch; and the shift-by-wire switch test device, system and method have stable repeatability and wide application range.

The invention discloses a preparation method of an ambroxol hydrochloride impurity reference substance. The method comprises the following steps: firstly, dissolving ambroxol hydrochloride in an appropriate amount of dichloromethane solution, then adding a quantitative aqueous solution of sodium hydrogencarbonate for uniform stirring to obtain free ambroxol, separating, drying and concentrating the organic phase, dissolving the concentrated solution in the alcohol solution, then refluxing with excess orthoformate to obtain a crude product of (1R, 4R)-4-(6,8-dibromo-3,4-dihydroquinazoline)-cyclohexanol after condensation, and obtaining a pure product after the crude product is purified by silica gel column chromatography. The invention has the creative application of orthoformate as a condensing agent. Compared with other condensing agents reported in the literature, the orthoformate has the advantages such as good stability, high safety, low cost, high product yield and easy purification.

The invention discloses a recombinant canine sourced antibody scFv-Fc against the H3N2 canine influenza virus (H3N2 CIV). The recombinant canine sourced antibody scFv-Fc is prepared by the following steps: extracting the total RNA of an infection cell and performing reverse transcription to obtain cDNA; amplifying VH, VL and Fc genes; performing PCR fusion, and extracting recombinant plasmids T-scFv and/or T-CE-Fc; performing amplifying to obtain scFv and/or Fc on the basis of the T-scFv and/or T-CE-Fc, linking the scFv and/or Fc target fragments into a cloning vector, and selecting a positivestrain; performing amplification by using the scFv as a template and CE-scFv-F and/or CE-scFv-R as a primer, and performing homogenous recombinant linkage on the product and the vector, transforminga Rosetta competent cell, and selecting a positive strain named ET-32a-scFv-Fc after resistance plate screening. The recombinant canine source antibody scFv-Fc can be used for preparing similar biological preparations. The recombinant canine sourced antibody against H3N2 sub-type CIV lays a material foundation for preventing and controlling canine infection caused by the H3N2 CIV and dealing withpotential menace of the H3N2 CIV on human health and public hygiene.