Lily virus gene chip and preparation method thereof as well as method for detecting lily viruses using lily virus gene chip

Abstract

The invention discloses a lily virus gene chip and a preparation method thereof as well as a method for detecting lily viruses using the lily virus gene chip. The lily virus gene chip includes a solid phase carrier and an oligonucleotide probe fixed on the carrier, wherein the probe is selected from nucleotide segments corresponding to one or more virus genes of lily symptomless virus (LSV), cucumber mosaic virus (CMV) or lily mottle virus (LMoV). The viruses detected by the lily virus gene chip provided by the invention include three types of viruses which have severe impact and damage on cut lily flowers. The method for detecting lily viruses using the lily virus gene chip, provided by the invention has the advantages that specific primers are used for PCR amplification; amplification products are fluorescence-labeled, with high accuracy; the gene chip is high in sensitivity; a probe with specificity is selected to hybridize with the amplification products, with high specificity; the defect that a solution is false positive when the PCR products are detected through gel electrophoresis can be avoided; the detection is fast; the method is good in stability and low in cost, and is applicable to an accurate detection on a large number of lily samples in short time.

Description

technical field [0001] The invention relates to a plant virus gene chip and a detection method thereof, in particular to a gene chip capable of simultaneously detecting multiple lily viruses and a detection method thereof, belonging to the field of microbial detection. Background technique [0002] Lily (Lilium) is an important economic crop that combines ornamental, edible and medicinal uses, and has become one of the most important cut flowers and ornamental flowers in the world. In recent years, with the growing area of ​​lily planting, long-term asexual reproduction has caused the accumulation of viruses; viral diseases have become more and more harmful to lily, affecting the yield and quality of lily, and causing huge economic losses to the lily planting industry. In the late 19th century, viral diseases nearly drove lily cultivars and varieties to extinction. Due to the different sources of bulbs of lily cultivated in various regions, and most of them lack strict plan...

AI Technical Summary

Problems solved by technology

The practical application of these technologies has cumbersome purification processes and takes a long time; some cumbersome operations are prone to contamination and false positives; the advantages of electron microscope identification of plant viruses are fast, simple and direct, and can directly observe the virus morphology and structure And the ultrastructural changes of cells caused by the virus infecting the host, but the operation of the electron microscope requires certain skills, and the work is relatively concentrated, and the number of samples is not easy to be too large; and these detection methods have a large workload, long time, and poor sensitivity, making it difficult to detect a large number of samples

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