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A kind of rooting method of silver birch tissue culture seedling outside the bottle

A tissue culture seedling and exogenous rooting technology is applied in the field of artificial seedling raising of new tree species for ecological management. The effect of planting survival rate, speeding up seedling reproduction and reducing cultivation space

Inactive Publication Date: 2016-01-13
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Due to the low seed setting rate and germination rate of silver birch, cutting propagation is very difficult, and the rooting rate in the tissue culture propagation bottle is only about 20%, the cultivation period is long, the transplanting survival rate is low, the production cost is high, and it is difficult to adapt to the needs of a large number of seedlings. develop

Method used

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  • A kind of rooting method of silver birch tissue culture seedling outside the bottle
  • A kind of rooting method of silver birch tissue culture seedling outside the bottle

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1 MS culture medium formula is prepared by dissolving in water according to the ratio of the type and quality of medicines containing the following nutritional elements per 1 liter, and the pH is adjusted to 5.8

[0026]

[0027] Add water to 1000ml, additionally add 6-benzyl adenine 0.5-3.0mg / l, naphthalene acetic acid 0.01-0.2mg / l.

Embodiment 2

[0028] Example 2 The MS medium formula is prepared by dissolving in water according to the ratio of the type and quality of medicines containing the following nutrient elements per 1 liter, and the pH is adjusted to 5.8

[0029]

[0030] Add water to 1000ml, additionally add: 6-benzyl adenine 0.2-2.0mg / l, naphthalene acetic acid 0.01-0.2mg / l.

Embodiment 3

[0031] Embodiment 3 carries out according to the following steps:

[0032] (1) Establishment of sterile culture: Select healthy and excellent trees, pick young shoots free from pests and diseases, and without mechanical damage, trim them into stems with 1-2 axillary buds, wash them with running water, and use 0.1 % mercuric chloride for 3 minutes, and then washed 4 times with sterile water, one section of a bottle was inoculated on MS+6BA2mg / l+NAA0.05mg / l medium, cultivated for 30 days, green shoots sprouted from the leaf axils, Cut the green shoots and inoculate them on the proliferation medium, and after subculture, they can grow and reproduce continuously to complete the establishment of sterile cultures;

[0033] (2) Cultivation of rootless seedlings: Inoculate the sterile propagules into the proliferation medium of MS+6BA0.5mg / l+NAA0.05mg / l, place them at room temperature 28°C, and cultivate them in a culture room with light intensity of 1500lx for 30 Days, the multiplic...

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Abstract

The invention discloses an out-of-tube rooting method for tissue culture seedlings of silver birch. The out-of-tube rooting method comprises the following seven steps: establishing an axenic culture medium; culturing the tissue culture seedlings; acclimatizing rootless vitro seedlings; transplanting; managing after transplanting, and the like. According to the method provided by the invention, the current situation of low vitro rooting percentage of the silver birch is solved, the vitro rooting link is simplified, the survival rate of transplanting is increased and the growing condition is excellent, so that the culturing time of tissue culture seedlings is shortened, the seedling multiplying speed is increased, the culturing space is reduced, the production link is simplified and the production cost is saved. The reliable technical guarantee is supplied to the industrialized production according to the out-of-tube rooting method for the tissue culture seedlings of the silver birch provided by the invention.

Description

technical field [0001] The invention relates to a method for rooting out of a bottle of tissue cultured seedlings of tree species, in particular to a method for rooting out of a bottle of tissue cultured seedlings of silver birch, belonging to the technical field of artificial seedling cultivation of new tree species for ecological management. Background technique [0002] Silver birch is native to Oceania. It is evergreen, with straight trunk and beautiful tree shape. Especially in the flowering season, the green bushes are lined with orange-yellow flowers. It is a landscape tree and street tree. It is introduced in various cities and towns in Guangdong, Guangxi, and Yunnan. One of the main greening tree species in the city, there are about 60 genera and 1,300 species of silver birch, such as safflower silver birch, horse felt silver birch, super silver birch, Jones silver birch, Canberra silver birch, ground cover silver birch, bronze Silver birch, moonlight silver birch, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G31/00
Inventor 杨小红黄少伟刘天颐刘纯鑫张
Owner SOUTH CHINA AGRI UNIV
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