Drug carrier capable of realizing drug delivery specifically targeting tumor and application thereof

A tumor-targeting and drug-based technology, which is applied in the interdisciplinary fields of bionics, nanotechnology, immunology and biomedicine, can solve the problems of limiting the use of chemotherapy drugs, lack of targeting, side effects, etc., and achieves good application prospects and huge The effect of social and economic benefits

Active Publication Date: 2014-09-03
CHINA SCI XINYUN BIOTECH BEIJING CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chemotherapy and anticancer drugs commonly used in clinical practice have serious side effects due to lack of targeting
And these side effects often limit the use of chemotherapy drugs and lead to many cancers losing treatment options
For example, severe side effects caused by most anthracycline antineoplastic drug molecules and non-specific toxicity of platinum-containing substances greatly limit the use of these chemotherapy drugs

Method used

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  • Drug carrier capable of realizing drug delivery specifically targeting tumor and application thereof
  • Drug carrier capable of realizing drug delivery specifically targeting tumor and application thereof
  • Drug carrier capable of realizing drug delivery specifically targeting tumor and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1. Preparation and characterization of tumor-targeted nano-drug carrier HFn-Dox

specific Embodiment

[0041] As a drug carrier, the first thing to solve is the problem of drug loading. As mentioned above, the currently commonly used method is to load drugs into protein cavities by depolymerization-polymerization of protein shells under pH control. In fact, because of the stability of H-ferritin, it will depolymerize only under the condition of pH=2. Treating the ferritin protein shell under such strong acidic conditions will cause the ferritin subunits to fail to completely reassemble into the original protein shell when the pH value returns to neutral conditions, but will cause some "gap" ( Kim, M. et al., Biomacromolecules, 2011). This will cause the ferritin nanoparticles to be unstable after the drug is loaded, and the drug-encapsulation yield will decrease. Moreover, the incompleteness of the protein shell will affect its targeting to tumors. Another method of loading drugs into ferritin is to use metal ions as drug additives. After chemotherapy drugs are combined with...

Embodiment 2

[0046] Example 2. Research on in vitro release and stability of drug-loaded nano drug carrier HFn-Dox

[0047] (1) In order to study the stability of the targeted drug carrier HFn-Dox loaded with drugs, we put HFn-Dox nanoparticles (500 μmol / L, 500 μL in Dox amount) into the dialysis tube D-tube (MWCO 6-8kDa, Novagen), and then placed in normal saline or normal mouse serum for incubation at 37°C. Samples were taken at incubation times of 0, 1, 2, 4, 8, 12, 24, 36, 48, and 60 hours, and the amount of doxorubicin was determined by HPLC. The results of the assay are expressed in percent dissociation. Drug retention percentage = 100% - dissociation percentage.

[0048] The result is as figure 2 As shown in a, the results of the stability test show that the HFn-Dox drug carrier system is very stable in mouse serum at 37°C. Less than 10% dissociated after 60 hours. This indicated that HFn-Dox could stably exist in mouse serum after administration to mice through tail vein.

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Abstract

The invention discloses a tumor-targeting drug delivery system, and the system comprises a tumor-targeting drug carrier and a tumor-treating drug, wherein the tumor-targeting drug carrier comprises full heavy-chain human ferritin. The invention also discloses a preparation method for the tumor-treating drug delivery system. The method comprises: depolymerizing polymerized full heavy-chain human ferritin; adding the tumor-treating drug into the depolymerized full heavy-chain human ferritin, so as to enable depolymerized full heavy-chain human ferritin to be combined with the tumor-treating drug; and polymerizing the depolymerized full heavy-chain human ferritin combined with the tumor-treating drug again to form nano-particles.

Description

technical field [0001] The invention belongs to the intersecting fields of nanotechnology, bionics, immunology and biomedicine. In particular, the present invention discloses a tumor-targeted drug delivery system, which includes a tumor-targeted drug carrier and a tumor treatment drug, wherein the tumor-targeted drug carrier contains full heavy chain human ferritin. The invention also discloses a preparation method of the tumor targeting drug delivery system. Background technique [0002] Cancer has become one of the major diseases that are increasingly common and seriously threaten human life and quality of life. As the aging population continues to increase, the incidence of cancer is expected to continue to grow. Currently, there are three main methods of treating cancer: surgery, radiotherapy and chemotherapy. The choice of cancer treatment depends on the type, location, and spread of the cancer. Chemotherapy is a treatment method that uses chemical drugs to kill tum...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/51A61K47/42A61P35/00
CPCA61K47/64A61K47/6925A61K31/704A61P1/04A61P35/00A61P35/02A61K47/42A61K9/51A61K31/70
Inventor 阎锡蕴范克龙梁敏敏郑继燕冯静杨东玲
Owner CHINA SCI XINYUN BIOTECH BEIJING CO LTD
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