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A method for breeding a three-line sterile line of hybrid rice with white midribs on leaves

A three-line male sterile line and hybrid rice technology, applied in the field of hybrid rice three-line male sterile line breeding, can solve the problem of reducing rice photosynthetic efficiency, affecting the growth period of rice male sterile line, agronomic traits, and greatly affecting rice photosynthesis and other problems, to achieve the effects of good plant and leaf morphology, excellent agronomic traits, and improvement of the purity of sterile lines and hybrids

Active Publication Date: 2015-07-29
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, it is worth noting that the existing leaf color markers such as green-turning albino leaf color, purple leaf, yellow leaf, light green and striped vein markers are expressed in large areas in leaves, which have a great impact on the photosynthesis of rice and reduce the The photosynthetic efficiency of rice, which more or less affects the growth period and agronomic traits of rice and even the cultivated sterile lines

Method used

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  • A method for breeding a three-line sterile line of hybrid rice with white midribs on leaves
  • A method for breeding a three-line sterile line of hybrid rice with white midribs on leaves
  • A method for breeding a three-line sterile line of hybrid rice with white midribs on leaves

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1 Breeding of Rice Carrying Leaf Midrib as White Mark

[0030] (1) Get 3000 dry seeds of "Ningjing No. 3" with a moisture content of 13%, carry out cobalt-60 gamma ray mutagenesis, dose 250Gy, dose rate 10Gy / min, get mutagenic seeds (M 0 ).

[0031] (2) direct seeding mutation seeds, mixed with the seeds of all mutation plants (M 1 ).

[0032] (3) The seeds obtained in step (2) are planted and transplanted by individual plants, and 100,000 individual plants are planted in the field, and 5 mutant individual plants (M 2 ).

[0033] (4) The above-mentioned 5 variation single plants are planted by the single plant of the strain, observe the stability of the midrib of the leaf between the strain and the single plant for the white trait, and screen 4 stable strains (M 3 ).

[0034] (5) Evaluate the expression of the white midrib of the leaves at different development stages, and screen the lines whose midrib is expressed in the whole growth period of the white tr...

Embodiment 2

[0043] Example 2 Breeding of Hybrid Rice CMS Line and Maintainer Line Carrying Leaf Midrib as White Mark

[0044]Breeding material:

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Abstract

The invention discloses a breeding method of a hybrid rice three-line sterile line with leaves carrying white midribs. The breeding method comprises the steps: with Zhe midrib rice as a male parent and a rice variety of the three-line sterile line as a female parent, selfing after hybridizing to obtain F2; performing selfing on plants with white midribs and excellent characters, which are selected from the F2, performing continuous selfing on plants which have white midribs and excellent characters, are capable of keeping the sterility characteristics of the three-line sterile line, and are selected from the selfed later generation to obtain a hybrid rice three-line maintainer line with leaves carrying white midribs; with the hybrid rice three-line maintainer line as a male parent, performing test crossing with a rice variety of the three-line sterile line; performing back-crossing on sterile plants selected from the later generation obtained through test crossing, and the hybrid rice three-line maintainer line; with the hybrid rice three-line maintainer line as a cycle parent, performing continuous back-crossing on sterile plants with white midribs, which are selected from the later generation and obtained through back-crossing, and the cycle parent to obtain the hybrid rice three-line sterile line with leaves carrying white midribs. In the sterile line obtained by the invention, the marked characters are expressed in the midribs of the leaves only, without influencing the economical characters; hybrids can be simply rejected, and the purity can be identified.

Description

technical field [0001] The invention relates to the technical field of crop genetics and breeding, in particular to a method for breeding a three-line male sterile line of hybrid rice with leaves carrying white midribs. Background technique [0002] The annual planting area of ​​hybrid rice in my country accounts for about half of the total planting area of ​​rice. High-purity and high-quality hybrid rice seeds are the basic guarantee for a large number of rice farmers to harvest. It is one of the breeding goals of breeders to cultivate excellent agronomic traits and to be simple, controllable and pure in the production of hybrids. For this reason, breeders design to combine leaf color markers with breeding, introduce leaf color markers into sterile lines, and cultivate sterile lines with leaf color markers, so as to facilitate the removal of self-bred seeds and hybrids according to the significant difference in leaf color , to achieve the goal of easy identification of hy...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/02
Inventor 吴殿星舒小丽张宁王寅孙健柳蕾
Owner ZHEJIANG UNIV
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