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Amino acid sequence for detecting autoantibody of tumor immune marker BIRC5 and applicaiton

A tumor marker and autoantibody technology, which is applied in the field of preparation of early diagnosis reagents for tumors and the development of targeted drugs for the treatment of tumors, can solve problems such as undetectable, and achieve the effect of high specificity and high sensitivity

Inactive Publication Date: 2014-10-08
SHENZHEN YINGJI BIOLOGICAL SCI & TECH DEV CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

BIRC5 is highly expressed in tumor cells and human embryonic cells, but hardly detected in differentiated mature cells

Method used

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  • Amino acid sequence for detecting autoantibody of tumor immune marker BIRC5 and applicaiton
  • Amino acid sequence for detecting autoantibody of tumor immune marker BIRC5 and applicaiton
  • Amino acid sequence for detecting autoantibody of tumor immune marker BIRC5 and applicaiton

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] kit preparation

[0037] 1 Reagents See Tab.2-9 for reagent preparation.

[0038] Tab.2 Antigen coating buffer Sodium azide 0.1g PBS (0.01M, pH7.4) 100ml Store at 4°C

[0039]

[0040]

[0041]

[0042]

[0043]

[0044]

[0045]

[0046]

[0047] 2 operations

[0048] (1) Coating: the working antigen and reference antigen are diluted to the working concentration with coating solution, and coated on the microtiter plate,

[0049] overnight at 4°C.

[0050] (2) Add plasma (primary antibody): Wash the ELISA plate 3 times with washing buffer, dilute the plasma to an appropriate concentration with the analysis solution, generally 1:100-1:500, 100 μl per well, incubate at 25°C or room temperature 2~3h;

[0051] (3) Secondary antibody incubation: wash with washing buffer for 3 to 5 times, dilute the secondary antibody standard solution IgG with the analytical solution, add 200 μl to each well, and incubate at 25°C / room temperatur...

Embodiment 2

[0055] Detection of BIRC5 auto-IgG antibody in patients with lung cancer

[0056] Sample collection: 271 plasma samples of lung cancer patients (including 113 cases of phosphorus carcinoma and 176 cases of adenocarcinoma) and 227 serum samples of age- and sex-matched healthy individuals were collected for lung cancer research. The clinical diagnosis of tumor patients conforms to the International Classification of Diseases standard ICD-10. The healthy group and the lung cancer group were matched in gender and age and were comparable (P>0.05)

[0057] Lung cancer detection results: According to Tab.10, the area under the ROC curve (AUC) of BIRC5 antibody IgG in serum of lung cancer patients is 0.66, the sensitivity is 37.8%, and the specificity is 90.4%; the level of BIRC5 antibody IgG in plasma of patients is significantly higher than that of healthy people group (Z=4.74, P<0.0001). The above data fully show that the autoantibody IgG level of lung cancer patients detected by...

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PUM

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Abstract

An amino acid sequence for detecting autoantibody of tumor immune marker BIRC5 and an application belong to the technical field of immunology. The invention provides an antigen amino acid sequence of apotosis inhibition gene BIRC5. A BIRC5 polypeptide antigen is used for detecting corresponding specific autoantibody in blood of lung cancer patients, wherein the autoantibody can be used as a tumor marker for evaluating risk of generation of lung cancer. The polypeptide antigen and an antibody thereof can be used for preparing a tumor early-stage diagnosis reagent and developing a targeted drug which is used for treating tumors.

Description

[0001] technical field [0002] The invention belongs to the technical field of immunology, and relates to the preparation of tumor early diagnosis reagents and the development of targeted drugs for treating tumors. Background technique [0003] A large number of studies have shown that tumor-associated antigens in serum or plasma can induce the body to produce autoantibodies, and there are both tumor antigens and autoantibodies against the tumor antigens in the serum of cancer patients. Therefore, both antibodies can be used to detect tumor antigens, and antigens can also be used to detect tumor autoantibodies. However, in the early stages of tumorigenesis, the specificity and sensitivity of using tumor autoantibodies to detect tumors are much higher than using tumor antigens to detect tumors, because the expression of tumor antigens can only reach a detectable level in the middle and late stages. In addition, many tumor-associated antigens exist not only in tumor patients...

Claims

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Application Information

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IPC IPC(8): C07K14/47G01N33/68G01N33/574G01N33/564
CPCC07K14/4747G01N33/57423G01N2333/47
Inventor 孙世龙张聪刘颖尉军
Owner SHENZHEN YINGJI BIOLOGICAL SCI & TECH DEV CO LTD
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