Serum-free medium for hybridoma cells

Abstract

The invention discloses a serum-free medium for hybridoma cells. By adding various amino acids, vitamins, microelements and supplementing factors such as alkaloid, fatty acid and hormones, the medium makes the hybridoma cells quickly grow in a suspension culture condition without adaptation. Both the cell density and cell viability can be equivalent to those in a serum medium. The serum-free medium for hybridoma cells not only can satisfy the requirements on culture of hybridoma cells, but also effectively avoids various adverse factors caused by use of serum. The production cost is lowered, and the serum-free medium for hybridoma cells can completely replace the serum medium for high density large-scaled culture of hybridoma cells.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a serum-free culture medium for hybridoma cells. Background technique [0002] The serum in the traditional serum-containing medium brings many unfavorable factors to production and scientific research. The main function of serum is to provide cells with hormones, growth factors, transfer proteins and other nutrients needed for growth and value-added, but it has many disadvantages: there are differences between batches, a lot of verification work is required, it is inconvenient to use, the composition is not clear, there are Growth-inhibiting components are especially not conducive to the separation and purification of target products such as vaccines and monoclonal antibodies, and are easily infected by viruses and mycoplasma; expensive serum may cause shortages in supply, and the source of serum is unstable. [0003] Hybridoma cells are cells that are fused with myeloma cells and ...

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