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A colloidal gold immunochromatographic test strip for detecting influenza A antigen and method for detecting influenza A antigen

A technology of immunochromatographic test paper and colloidal gold, which is applied in the field of colloidal gold immunochromatographic test strips and the use of the test strips to detect influenza A antigens, colloidal gold immunochromatographic test strips and the detection of influenza A antigens, can solve the problem of The reading results are not easy to obtain, the operation is complicated, and the sensitivity is poor, etc., to achieve the effect of fast and intuitive reading results, high overall coincidence rate, and improved detection sensitivity

Active Publication Date: 2016-05-18
THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The above-mentioned relevant literatures related to the detection of influenza antigens generally have defects such as complicated operation, poor sensitivity, and difficult to obtain reading results. Therefore, it is of great clinical diagnostic significance to find a detection method with high sensitivity, fast and intuitive reading results, etc.

Method used

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  • A colloidal gold immunochromatographic test strip for detecting influenza A antigen and method for detecting influenza A antigen
  • A colloidal gold immunochromatographic test strip for detecting influenza A antigen and method for detecting influenza A antigen
  • A colloidal gold immunochromatographic test strip for detecting influenza A antigen and method for detecting influenza A antigen

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Experimental program
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Effect test

Embodiment 1

[0059] Sources of reagents and equipment used:

[0060] 1 Influenza A, antigen and its antibody: Beijing Mozhidong Biotechnology Co., Ltd.;

[0061] 2 Goat anti-mouse IgG: Beijing Boaosen Biotechnology Co., Ltd.;

[0062] 3 streptavidin, biotin: Beijing Boaosen Biotechnology Co., Ltd.;

[0063] 4 Nitrocellulose membrane: Merck Millipore;

[0064] 5. Three-dimensional planar film marking instrument, three-dimensional planar gold spraying instrument: Shanghai Jinbiao Biotechnology Co., Ltd.;

[0065] 6 Freeze dryer: Beijing Boyikang Experimental Instrument Co., Ltd.;

[0066] 7 Slitting machine: Shanghai Gold Standard Biotechnology Co., Ltd.;

[0067] 8PVC rubber sheet, absorbent paper, glass fiber membrane, and card case were purchased from Shanghai Jinbiao Biotechnology Co., Ltd.

[0068] Detection of Influenza A Virus Antigen:

[0069] 1. Preparation of streptavidin-gold-antibody conjugates

[0070] (1) Take 200 μg of influenza A labeled antibody and place it in a dial...

Embodiment 2

[0080] The influenza A colloidal gold test strips of Guangzhou Wanfu Biotechnology Co., Ltd. and Kabili Biotechnology Co., Ltd. are compared with the method of the present invention, and by detecting different concentrations of influenza A antigens, the results are as follows Figure 9 Shown, the sensitivity of the colloidal gold test strips of Wanfu and Kaybile is respectively 25ppb and 50ppb, and the detection sensitivity of the method of the present invention is 12.5ppb, illustrates that the colloidal gold immunochromatography test strip of the present invention has higher sensitivity.

Embodiment 3

[0082]Dilute the influenza A antigen with human serum to simulate the detection of actual samples, and dilute solutions with different concentrations. 10 actual samples randomly checked were detected with the test strip prepared by the present invention. Drop the sample on a test strip and read the result after 15 minutes. The result is as Figure 10 As shown, all 10 samples tested in the spot check showed positive results.

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Abstract

The invention relates to a colloidal gold immunochromatography test strip used for testing an H1N1 influenza antigen and a method for testing the H1N1 influenza antigen. The test strip comprises a base plate, a sample pad, a conjugate pad, a pyroxylin film and an absorbent paper, wherein the sample pad, the conjugate pad, the pyroxylin film and the absorbent paper are lapped and adhered on the base plate sequentially; the conjugate pad is a combination of a gold-label-biotin conjugate pad and a streptavidin-gold-antibody conjugate pad; according to the method for testing the H1N1 influenza antigen, a sample to be tested is dropwise added on the test strip; if a red band is displayed at a testing line (1), the sample to be tested contains the H1N1 influenza antigen. According to the advantages, the test strip provided by the invention is cheap in price, fast, simple, convenient, and greatly suitable for on-site testing; moreover, the test strip can meet the requirement of higher testing sensitivity, and has an important clinical diagnostic significance.

Description

technical field [0001] The invention belongs to the technical field of immune detection and analysis. It specifically relates to a colloidal gold immunochromatographic test strip and a method for detecting influenza A antigen, in particular to a colloidal gold immunochromatographic test strip based on signal enhancement and a method for detecting influenza A antigen using the test strip. Background technique [0002] Influenza (abbreviated as influenza) is an acute respiratory infection caused by influenza virus, and it is also a disease with strong contagion and fast transmission speed. It is mainly spread through droplets in the air, person-to-person contact or contact with contaminated items. Typical clinical symptoms are: sudden onset of high fever, general pain, significant fatigue and mild respiratory symptoms. Generally, autumn and winter are the high-incidence period, and the complications and death caused by it are very serious. [0003] Influenza is influenza ca...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/569
CPCG01N33/558G01N33/56983G01N2333/11
Inventor 蒋兴宇曹丰晶张伟陈翊平陈雯雯赵大龙
Owner THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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