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A test paper and a method for simultaneously detecting IgG and IGM in liquid samples

A liquid sample and test paper technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of missed detection, long time required for detection, false negatives, etc., and achieve a high overall coincidence rate, fast and intuitive reading results, highly specific effect

Active Publication Date: 2016-08-24
THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The current detection techniques for detecting specific IgM and IgG in samples through enzyme-linked immunoimmunoassay kits require separate detection of IgM and IgG, resulting in a long time for detection (detection by ELISA method generally takes several hours)
If you simply detect specific IgM and IgG at the same time, there will be defects such as missed detection and false negative

Method used

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  • A test paper and a method for simultaneously detecting IgG and IGM in liquid samples
  • A test paper and a method for simultaneously detecting IgG and IGM in liquid samples
  • A test paper and a method for simultaneously detecting IgG and IGM in liquid samples

Examples

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preparation example Construction

[0034] The preparation method of colloidal gold test paper strip of the present invention specifically can comprise the steps:

[0035] (1) Put 150-250 μg of antigen in a dialysis bag, place the dialysis bag in 150-200 mL of Tris-HCl buffer solution with a concentration of 5-10 mM for dialysis, and replace the buffer solution every 2h-3h for co-dialysis 18-24h, after the dialysis is completed, take out the retained solution and place it in a centrifuge tube, add 2-3mL triple distilled water and centrifuge at 10000-15000rpm for 10-25min, then discard the precipitate to obtain the centrifuged antigen;

[0036] (2) Add 500-1500mL triple-distilled water into the beaker, then add 10-40mL of 1% by weight sodium citrate solution, heat to boiling, then add 5-15mL of 0.5-1.5% by weight of chloroauric acid solution After boiling for 15min-20min and cooling to 15-50℃, take 15-25mL of the colloidal gold solution in a beaker, add 150-160μL of 0.05-0.15M K 2 CO 3 Adjust the pH of the solu...

preparation example 1

[0076] This preparation example is used to illustrate the test paper that utilizes the preparation method of test strip provided by the present invention to prepare for detecting anti-Mycoplasma pneumoniae IgG and IgM in the liquid sample:

[0077] (1) Put 200 μg of Mycoplasma pneumoniae antigen in dialysis bags, place the dialysis bags in 200 mL of Tris-HCl buffer solution with a concentration of 5 mM for dialysis, and change the buffer solution every 2.5 hours for a total of 24 hours of dialysis. After completion, the retained solution in the dialysis bag was taken out and placed in a centrifuge tube, 2 mL of triple-distilled water was added and centrifuged at 10,000 rpm for 10 min, and the precipitate was discarded to obtain the post-centrifugation antigen.

[0078] (2) Add 1000mL triple distilled water into the beaker, then add 20mL of 1% by weight sodium citrate solution and heat to boiling, then add 10mL of 1% by weight chloroauric acid solution, boil for 15min and cool t...

preparation example 2

[0083] Test strips for detecting specific IgG and IgM anti-Chlamydia pneumoniae antigens were prepared according to the same method as Preparation Example 1.

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Abstract

The invention discloses test paper. The test paper comprises a sample adding region, a binder region, an observation region and a water absorption region, which are sequentially arranged, wherein the binder region contains antigen combined colloidal gold which can be eluted by a liquid sample; the observation region comprises antibody lines, an antigen line T3 and a quality control line C in sequence from the binder region to the water absorption region; the antibody lines comprise a T1 line and a T2 line; the antibody line T1 is fixedly provided with an anti-human IgG antibody, the antibody line T2 is fixedly provided with an anti-human IgM antibody, the antigen line T3 is fixedly provided with by an antigen, and the quality control line C is fixedly provided with an anti-antigen antibody; and the IgG and the IgM can be specifically combined with the antigen. The test paper can detect whether the tested sample contains IgG and IgM simultaneously one time, can prevent leaked detection, shorten the detection time and reduce the detection expense and can be convenient for field detection.

Description

technical field [0001] The invention relates to a test paper and a method for simultaneously detecting IgG and IgM in a liquid sample. Background technique [0002] Immunoglobulin M (IgM) and immunoglobulin G (IgG) are the two most important antibodies in humans or animals. When the body is infected with an antigen, IgM is the first immunoglobulin produced in the humoral immune response induced by antigen stimulation, which can bind complement and is mainly distributed in serum. Because IgM has a high binding value, it is a high-efficiency anti-biological antibody and plays an important role in the early defense of the body. The appearance of IgG antibody is later than that of IgM antibody. It is the most persistent and important antibody in the primary immune response, and it exists only in monomeric form. Most antibacterial, antiviral and antiviral antibodies belong to IgG, and IgG plays a major role in fighting infection. Therefore, by detecting the presence or absence...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/558
CPCG01N33/6854
Inventor 蒋兴宇刘煜凯曹丰晶张伟
Owner THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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