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A kind of immunochromatographic test strip, detection method and application

An immunochromatographic test strip and antibody detection technology, applied in the field of immunodetection, can solve the problems such as the inability to meet the high sensitivity and quantitative detection of the target, only qualitative or semi-quantitative detection, poor sensitivity, etc. Intuitive, quantitative detection, low-cost effect

Active Publication Date: 2017-04-26
THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The immunochromatographic test strips generally use colloidal gold or magnetic beads as tracer markers for visual detection with the naked eye, but this method has poor sensitivity and can only be used for qualitative or semi-quantitative detection, which cannot meet certain requirements. Requirements for high sensitivity and quantitative detection of target objects
[0005] Therefore, the technology that Janus particles are used for immunochromatographic test strips is needed in this area to solve the poor sensitivity and quantifiable defects of traditional colloidal gold immunochromatographic test strips

Method used

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  • A kind of immunochromatographic test strip, detection method and application
  • A kind of immunochromatographic test strip, detection method and application
  • A kind of immunochromatographic test strip, detection method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Example 1: Detection of alpha-fetoprotein

[0053] We take alpha-fetoprotein (AFP) as an example of macromolecular detection.

[0054] 1. Preparation of immunochromatographic test strips:

[0055]The Janus nanoparticles used in the experiment are gold nanoparticles-CdTe quantum dots (AuNPs (AuNPs) are used for visual detection by naked eyes, and CdTe quantum dots are used for fluorescence quantitative detection). Mycoavidin (Streptavidin, SA) and biotin (Biotin) were modified, and then through the specific reaction of streptavidin and biotin, the gold nanoparticles and CdTe quantum dots were successfully connected to finally generate gold Nanoparticles - Janus nanoparticles of CdTe quantum dots (refer to Sensitivity improved plasmonic goldnanoholes array biosensor by coupling quantum-dot for the detection of specific biomolecular interactions, Lihong Niu, Ke Cheng, Zuliang Du, Biosensorsand, 2013, 50, 137-142).

[0056] (1) Take 100 μg of AFP detection antibody in a d...

Embodiment 2

[0067] Embodiment 2: the detection of albuterol

[0068] We take albuterol as an example of small molecule detection, and the experimental design method is as follows:

[0069] 1. Preparation of immunochromatographic test strips:

[0070] (1) Take 100 μg of salbutamol detection antibody and place it in a dialysis bag to dialyze 5mM Tris-HCl for 24 hours. During this process, change the dialysate every 2 hours. After the dialysis is completed, take out the detection antibody and place it in a centrifuge tube Add triple distilled water to 2 mL, centrifuge and discard precipitated impurities;

[0071] (2) Prepare the conjugate pad: use K at a concentration of 0.01M 2 CO 3 Solution to adjust the pH of the Janus particle solution, stir evenly, then add the centrifuged antibody, stir for about 20 minutes, then add 2mL of 10% bovine serum albumin (BSA) solution, 10000rpm, centrifuge for 40min, then discard the supernatant, and then add 1% BSA solution (containing 1mM pH8.6 buffer...

Embodiment example 3

[0081] Implementation Case 3: Cd 2+ detection

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Abstract

The invention discloses an immunochromatographic test strip, a detection method by using the immunochromatographic test strip, and application of the immunochromatographic test strip; the immunochromatographic test strip comprises a conjugate cushion, a testing line and a quality control line; the conjugate cushion is coated with Janus nano-particles marked by a detection antibody; the testing line is coated with a capture antibody or antigen; and the quality control line is coated with an antibody of the detection antibody. On the basis of the Janus nano-particles, double functions including visual read-out and fluorescent read-out of a target object can be realized; compared with a traditional colloidal gold immunochromatographic test strip, for the immunochromatographic test strip, the quantitative detection can be realized; and the relatively high detection sensitivity can be obtained.

Description

technical field [0001] The invention relates to the technical field of immune detection, in particular to an immunochromatographic test strip, a detection method using the same and an application. Background technique [0002] Colloidal gold immunochromatographic test strips have been widely used in disease diagnosis, food testing, environmental monitoring and other fields because of their simplicity, convenience, speed, cheapness, and no need for large-scale instruments and professional operations. However, there are still some deficiencies in the colloidal gold-based immunochromatographic test strips, for example, it is difficult to achieve quantitative detection and high sensitivity (for example, alpha-fetoprotein AFP, etc.), which limits its detection in certain targets. aspects of application. [0003] Double-faced God particles, namely Janus particles, are asymmetric particles in appearance or properties. Due to the differences in physical and chemical properties, thi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/558G01N33/532G01N33/533
CPCG01N33/558
Inventor 蒋兴宇曹丰晶张伟
Owner THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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