Method for simultaneous determination of contents of 6 kinds of sedative-hypnotic drugs in serum by UHPLC-CAD technology

1. UHPLC-CAD, sedation and hypnosis technology, applied in the field of medicine, to achieve good clinical application prospects, improve drug efficacy, reduce side effects and drug costs

Active Publication Date: 2014-11-05
ZHEJIANG ACAD OF TRADITIONAL CHINESE MEDICINE
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  • Abstract
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  • Claims
  • Application Information

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According to the literature search, there are currently no literature reports or patent publications on the method for measuring the content of six sedative-hypnotic

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  • Method for simultaneous determination of contents of 6 kinds of sedative-hypnotic drugs in serum by UHPLC-CAD technology
  • Method for simultaneous determination of contents of 6 kinds of sedative-hypnotic drugs in serum by UHPLC-CAD technology
  • Method for simultaneous determination of contents of 6 kinds of sedative-hypnotic drugs in serum by UHPLC-CAD technology

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Embodiment Construction

[0047] A detailed description of the specific implementation of the present invention is given below.

[0048] UHPLC-CAD combined technology is used to simultaneously determine the content of 6 sedative and hypnotic drugs in serum. The method includes the following steps:

[0049] 1) Preparation of reference substance

[0050] Weigh accurately 45.79 mg of diazepam reference substance, 43.39 mg of lorazepam reference substance, 47.51 mg of clonazepam reference substance, 44.36 mg of estazolam reference substance, 43.60 mg of alprazolam reference substance, and Zolpidem tartrate Put 25.00 mg of the tan reference substance in a 100mL volumetric flask, add acetonitrile to dissolve, and quantify the concentration of 0.4579mg / mL diazepam reference substance solution, 0.4339mg / mL lorazepam reference substance solution, 0.4751 mg / mL clonazepam reference substance solution, 0.4436 mg / mL eszolam reference substance solution, 0.4360 mg / mL alprazolam reference substance solution and 0.2500 mg / m...

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Abstract

The invention relates to a method for simultaneous determination of the contents of 6 kinds of sedative-hypnotic drugs in serum by a UHPLC-CAD combined technology, wherein the 6 kinds of sedative-hypnotic drugs are diazepam, lorazepam, alprazolam, estazolam, clonazepam and zolpidem tartrate. The method is characterized by including the following steps: 1) reference substance preparation, 2) internal standard solution preparation, 3) sample solution preparation, 4) mobile phase solution preparation, 5) chromatographic condition setting, 6) electrospray detector condition optimization, 7) sample determination, 8) gradient-concentration reference substance solution preparation, 9) standard curve preparation, and 10) data calculation and analysis. The method is advanced, has good clinical application prospects and effects, can rationally use drugs for clinic, increases drug curative effects, lowers toxic and side effects and drug costs, and plays a positive role.

Description

Technical field [0001] The invention relates to a detection method that belongs to the field of medicines, in particular to a detection method for simultaneously determining six sedative and hypnotic drugs in serum by adopting a high-performance liquid phase-electrospray detection combined technology. Background technique [0002] In modern social life, due to fierce competition in work and tight life rhythm, various external stimulus factors affect people’s emotions, causing insomnia and tension, and then developing into various psychological and physical problems such as anxiety and depression, which have caused huge consequences for family and society. pressure. A foreign survey showed that 50%-60% of adults suffer from sleep disorders of varying degrees, and the incidence of insomnia in my country is as high as 40%. As the first-generation sedative and hypnotics, barbiturates have been basically replaced by other related drugs in recent years due to their adverse reactions s...

Claims

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Application Information

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IPC IPC(8): G01N30/88
Inventor 张扬陈炯寿旦马永春
Owner ZHEJIANG ACAD OF TRADITIONAL CHINESE MEDICINE
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