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Amniotic biological agent and preparation method thereof

A technology of biological preparations and amniotic membranes, applied in medical science, bandages, absorbent pads, etc., can solve problems such as allergic reactions, shorten wound healing time, etc., achieve the effect of reducing scar formation and improving healing time

Active Publication Date: 2014-12-10
CHENGDU QINGKE BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the problems existing in the prior art, an object of the present invention is to provide an amniotic membrane biological preparation, which solves the problems that the existing amniotic membrane preparations easily cause allergic reactions and are difficult to significantly shorten the wound healing time

Method used

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  • Amniotic biological agent and preparation method thereof
  • Amniotic biological agent and preparation method thereof
  • Amniotic biological agent and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] A kind of amniotic membrane biological preparation, is made up of the raw material of following ratio:

[0034] Decellularized amniotic membrane 1cm 2 : Platelet-rich factor plasma preparation 0.2ml;

[0035] The above-mentioned amniotic membrane biological preparation is obtained through the following preparations:

[0036] 1. Preparation of acellular amnion:

[0037] Take the fetal membranes of healthy people and animals, wash the blood stains on the surface of the fetal membranes with sterile phosphate buffer saline PBS, peel the amniotic membranes from the chorion, rinse them with PBS three times, and soak them in 1% Triton-100 for 24 hours;

[0038] After repeated washing with PBS, add 0.25% trypsin (containing 0.01% EDTA) dropwise to infiltrate the amniotic membrane, and enzymolyze it in a constant temperature incubator at 37°C for 4 hours;

[0039] Soak the amniotic membrane with 1~2 liters of PBS, and shake fully in a constant temperature shaking incubator at...

Embodiment 2

[0048] A kind of amniotic membrane biological preparation, is made up of the raw material of following ratio:

[0049] Decellularized amniotic membrane 1cm 2 : Platelet-rich factor plasma preparation 0.3ml;

[0050] The above-mentioned amniotic membrane biological preparation is obtained through the following preparations:

[0051] 1. Preparation of acellular amnion:

[0052] Amniotic membranes from healthy people and animals were washed with PBS for 3 times, and soaked in 1% Triton-100 for 18 hours;

[0053] After repeated washing with PBS, add 0.25% trypsin (containing 0.01% EDTA) dropwise to infiltrate the amniotic membrane, and enzymolyze it in a constant temperature incubator at 37°C for 2 hours;

[0054]Then soak the amniotic membrane with 1-2 liters of PBS, and fully shake in a constant temperature shaking incubator at 12°C and 180 rpm for 18 hours to obtain decellularized amniotic membrane.

[0055] 2. Preparation of platelet-rich factor plasma preparation: the pre...

Embodiment 3

[0062] A kind of amniotic membrane biological preparation, is made up of the raw material of following ratio:

[0063] Decellularized amniotic membrane 1cm 2 : Platelet-rich factor plasma preparation 0.5ml;

[0064] The above-mentioned amniotic membrane biological preparation is obtained through the following preparations:

[0065] 1. Preparation of acellular amnion:

[0066] Wash the blood on the surface of the fetal membrane with sterile phosphate buffered saline (PBS), peel the amniotic membrane from the chorion, rinse it with PBS three times, and soak it in 1% Triton-100 for 20 hours;

[0067] After repeated washing with PBS, add 0.25% trypsin (containing 0.01% EDTA) dropwise to infiltrate the amniotic membrane, and enzymolyze it in a constant temperature incubator at 37°C for 3 hours;

[0068] Then soak the amniotic membrane with 1-2 liters of PBS, and fully shake in a constant temperature shaking incubator at 8°C and a rotation speed of 190 rpm for 20 hours to obtain ...

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Abstract

The invention discloses an amniotic biological agent and a preparation method thereof. The amniotic biological agent is prepared by a freeze-drying, radiating and low-temperature storing platelet factor-enriched plasma preparation covering human and animal denuded amniotic membranes; and the amniotic biological agent is used for reparative therapy of skin injury in clinic. The amniotic biological agent can remarkably shortening healing time of a wound and relieving formation of scar on a wound surface; the amniotic biological agent is an industrial biological preparation with bright clinical application prospect.

Description

technical field [0001] The invention relates to medical biomaterial technology, in particular to an amniotic membrane biological preparation and a preparation method thereof. Background technique [0002] Acellular amnion has been widely used as a medical biomaterial, but in experiments we found that using acellular amnion alone to treat skin injuries (such as mechanical trauma, ulcers) can accelerate wound healing during the inflammatory response period, but compared with the control group The final healing time was not significantly improved compared to . [0003] The patent with application number 201310508091.5 discloses a biological amniotic membrane and its preparation method. The biological amniotic membrane adopts a collagen composite membrane, but the collagen has a certain immunogenicity, which is easy to cause allergic reactions in the body and has obvious side effects. Contents of the invention [0004] In view of the problems existing in the prior art, an obj...

Claims

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Application Information

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IPC IPC(8): A61L15/40A61L15/44
Inventor 康裕建丁雪琴陈静娴海泉赵峻
Owner CHENGDU QINGKE BIOTECH
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