HER-2 gene quick FISH detection kit

A detection kit, HER-2 technology, applied in the direction of microbial determination/inspection, biochemical equipment and methods, etc., can solve the problems of long hybridization time, large probe, unfavorable diagnosis and treatment, etc., to ensure reliability, The effect of reducing false positive results

Inactive Publication Date: 2014-12-10
GUANGZHOU HEAS BIOTECH CO LTD
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  • Claims
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Problems solved by technology

For non-repetitive gene sequences, the probe needs to cover the gene sequence other than the target gene, and the length of the probe is required to be relatively large, res

Method used

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  • HER-2 gene quick FISH detection kit
  • HER-2 gene quick FISH detection kit

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Embodiment 1

[0083] HER-2 gene rapid FISH detection kit, including FISH fluorescent probe, hybridization solution and staining solution, wherein the sequences of the probes are:

[0084] Chromosome 17 specific probe, its sequence is:

[0085] Probe 1: CATTGTCAGAAACTTCTTTGTGATG

[0086] Probe 2: ATTGCATTCAACTCACAGAGAGTTGA

[0087] Probe 3: AGGTTCCTTTTTCAAACAGCAGTTTC

[0088] Probe 4: CAATCACTCTTTCTGTGGAATCTGC

[0089] Probe 5: AAGTGGATATTTGGGCCTCTCTG

[0090] Probe 6: AGGATTTCGTTGGAAACGGGA

[0091] Probe 7: AACGGGATAACTGCACCTAACTAA

[0092] Probe 8: ACGGAAGCATTTCTCAGAAACTGCT

[0093] Probe 9: TTGTGATGATTGCATTCACCTCAC

[0094] Probe 10: AGAGTTGAACATTCCTATTGATAG

[0095] Probe 11: AGCAGTTTGGAAACACTCTTGTTG

[0096] Probe 12: CATAGAGTAGTTCTGAAACATGC

[0097] Probe 13: CCTGTGGTGGAAAACGAATTATG

[0098] Probe 14: ATATGTGGACCTCTCCGAAGATG

[0099] Probe 15: ATATGTGGACCTCTCCGAAGATG

[0100] Probe 16: CATTTCCAGAAACTTCTTGGTGATG

[0101] Probe 17: CCACTCTGTGGCCTTCGTTCG;

[0102] HER-2 gen...

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Abstract

The invention discloses a HER-2 gene quick FISH detection kit which comprises FISH fluorescent probes, a hybridization solution and a staining solution, wherein the sequences of the probes are respectively a No.17 chromosome specific probe and a HER-2 gene specific probe. By adopting the optimized short sequence fluorescent probes, the FISH detection time is shortened to 1-2 hours from 16-18 hours, and the kit can quickly and accurately detect the HER-2 gene and is beneficial to early diagnosis and treatment of diseases. By adopting two different types of probes, one probe is combined with the centromere of the No.17 chromosome, and the other probe is combined with the target gene HER-2, thereby better ensuring the reliability of the detection result and reducing the false positive result.

Description

technical field [0001] The invention relates to a FISH method and a detection kit thereof, in particular to a rapid FISH detection kit of HER-2 gene. Background technique [0002] The tyrosine kinase encoded by the Her2 gene - human epidermal growth factor receptor 2, contains 2 copies in normal tissues, and there are gene amplification and protein overexpression in 20%-30% of breast cancer patients. HER-2 amplification-positive breast cancer has strong invasiveness, short disease-free survival, and poor prognosis. Trastuzumab (Herceptin) is a recombinant DNA-derived humanized monoclonal antibody for HER-2 overexpressing breast cancer. Since only HER-2 overexpressing and gene-amplified breast cancer patients respond to trastuzumab, it is critical to correctly detect and assess the HER-2 status of breast cancer. Currently commonly used immunohistochemical methods cannot distinguish whether patients with moderately positive HER-2 protein (IHC detection 2+) have HER-2 gene am...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
CPCC12Q1/6841C12Q1/6876C12Q2600/16C12Q2600/166
Inventor 陈华云刘淑园肖湘文丁渭陈嘉昌黄爽曾烨黄家伟
Owner GUANGZHOU HEAS BIOTECH CO LTD
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