Application of low molecular weight basic fibroblast growth factor lmw FGF2

A technology of fibroblasts and growth factors, applied in the field of preparation of anti-hepatic fibrosis drugs, can solve problems such as unclear effects, achieve the effect of inhibiting the activation of hepatic stellate cells, inhibiting the activation of HSCs, and reducing liver fibrosis

Active Publication Date: 2016-04-13
HANGZHOU S EVANS BIOSCI LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, the effect of low molecular weight basic fibroblast growth factor LMWFGF2 on liver fibrosis remains unclear

Method used

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  • Application of low molecular weight basic fibroblast growth factor lmw FGF2
  • Application of low molecular weight basic fibroblast growth factor lmw FGF2
  • Application of low molecular weight basic fibroblast growth factor lmw FGF2

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1: Preparation of chronic liver injury mouse model

[0020] Utilize 10% (volume concentration) carbon tetrachloride (solvent is olive oil) to induce chemical liver damage to C57BL / 6J mice (body weight about 30 grams) of eight weeks of rat age, and each mouse is intraperitoneally injected with 0.25ml 10% CCl 4 , 2 times a week, continuous injection for 4 weeks, to establish the liver fibrosis model, and the injected mice were fed normally in a sterile environment.

Embodiment 2

[0021] Example 2: LMWFGF2 inhibits HSC activation and reduces liver fibrosis

[0022] LMWFGF2 reagent: LMWFGF2 (purchased from Peprotech) was dissolved in IMDM culture medium (purchased from Corning Company), and every 50 μg of LMWFGF2 was dissolved in 3.3 ml of IMDM culture medium.

[0023] Continuous injection of CCl as described in Example 1 4 For 4-week-old mice, LMWFGF2 was injected into the tail vein of the mice in the experimental group at a dose of 60 μg / kg body weight, and the control group was injected with IMDM culture medium twice a week for 1 week. During all mice continue to carry out CCl by the method described in embodiment 1 4 Liver injury, 3 days after the last injection of LMWFGF2, the mouse liver tissue was taken, and a part of the total RNA was extracted with TRizol (Invetigen). The extraction method was carried out according to the instructions, and then the reverse transcription kit RNAPCRkit (AMV) Ver3.0 (TaKaRa) was used reverse transcription. Realt...

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Abstract

The invention discloses application of a low molecular weight basic fibroblast growth factor LMW FGF2 in preparing medicament for resisting liver fibrosis. The low molecular weight basic fibroblast growth factor LMW FGF2 provided by the invention can inhibit the expression of HSCs activation marker genes alpha smooth muscle actin (alpha-SMA) and collagen type I [col I (alpha 1)], and thereby inhibiting the activation of hepatic stellate cells and decrease liver fibrosis.

Description

(1) Technical field [0001] The invention relates to the application of a low-molecular-weight basic fibroblast growth factor LMWFGF2, in particular to the application in the preparation of anti-hepatic fibrosis drugs. (2) Background technology [0002] Liver fibrosis and cirrhosis are the main pathological features of chronic liver diseases such as hepatitis and liver metabolic diseases, which seriously threaten health and life, and are major medical problems faced by human beings. In my country, 10%-15% of patients with hepatitis B, especially chronic active hepatitis B, may develop post-hepatitis cirrhosis within 5-10 years. In the development process of chronic liver disease of "chronic liver injury-hepatic fibrosis-cirrhosis", the prevention and treatment of liver fibrosis is the key, but there is still no effective method for this. Therefore, it is very urgent to develop effective anti-hepatic fibrosis drugs and methods. [0003] Liver fibrosis usually results from th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/18A61P1/16
Inventor 邵建忠潘若浪项黎新王萍刘小园
Owner HANGZHOU S EVANS BIOSCI LTD
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