Method and system for acquiring autosomal STR gene locus typing result of single individual from mixed seminal stains

Abstract

The invention provides a method and a system for acquiring an autosomal STR gene locus typing result of a single individual from mixed seminal stains. The method comprises the following steps of: acquiring different Y-STR gene loci; acquiring autosomal STR gene locus haplotype typing results of a sufficient amount of Y type spermoblast; merging the autosomal STR gene locus haplotype typing results of the Y type spermoblast, which have same typing results of the different Y-STR gene loci to obtain the autosomal STR gene locus typing result of the single individual from the mixed seminal stains. The invention also provides the system for acquiring the autosomal STR gene locus typing result of the single individual from the mixed seminal stains. The method and system which are provided by the invention can acquire the typing result of the single individual from the mixed seminal stains by comprehensively analyzing autosomal STR and Y-STR typing results, thereby providing a powerful technical support for a public security organ to fast determine a criminal suspect.

Description

technical field [0001] The present invention relates to a method and system for inferring typing, in particular to a method and system for obtaining the typing result of autosomal STR locus of a single individual from mixed seminal spots. Background technique [0002] Biological evidence extracted from sexual assault crime scenes is often a mixture of male and female bodily fluids, and in some cases different male bodily fluids (eg, mixed semen spots). The latter is often the key to the investigation and detection of criminal cases. At present, it is mainly through the analysis of the DNA of different male donors in mixed sperm spots to eliminate and determine criminal suspects. [0003] However, the current testing procedures for mixed sperm spots, such as the differential lysis method, can only remove female samples, and cannot separate sperm DNA from different male donors. Can be used to exclude but not identify individuals. In the follow-up, it is necessary to manually...

AI Technical Summary

Problems solved by technology

[0004] Existing studies have confirmed that the combined use of laser capture microdissection (LCM) and LV–PCR methods can obtain autosomal STR typing of single sperm, but because sperm cells are formed through meiosis and contain only half of the genetic material, it is difficult for mixed Autosomal STR typing of sperm cell DNA in seminal spots can only obtain a large number of different autosomal STR haplotype results, and it is difficult to distinguish which male the haplotype results are for, so the same identification cannot be made and individual identification

Y-STR typing is a commonly used typing method to determine paternal relationship and exclude suspects through Y chromosome locus typing. However, the sperm Y-STR locus typing of male individuals in the same paternal inheritance family is the same. Therefore, it is difficult to carry out the same identification and individual identification only by Y-STR locus typing

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