A positive control setting method for external micronucleus test of cigarette filter water extraction liquid

Abstract

The present invention relates to a method for setting the positive control of the external micronucleus test of the water extraction liquid of the cigarette filter. The method is as follows: 1) selecting the same number of cigarette filters meeting the conditions for preparing the positive control and the cigarette filter of the sample group to be tested; 2) The preparation concentration is 200-400? μg / ml cyclophosphamide aqueous solution; 3) Spray cyclophosphamide aqueous solution evenly on the cigarette filter tip used to prepare the positive control group, and wait for it to dry naturally for later use; 4) Use the same method for the positive control group and the test The sample group was extracted from the cigarette filter; 5) The extract was used as a solvent, prepared with powdered cell culture medium, and the prepared solution was filtered and sterilized; 6) The in vitro micronucleus test was carried out on the extract treated in step 5) . The positive control set by this method can verify whether the extraction process of the cigarette filter and the subsequent in vitro micronucleus test on the water extract of the cigarette filter are reliable.

Description

technical field [0001] The invention belongs to the technical field of tobacco, and in particular relates to a method for setting a positive control when conducting an in vitro micronucleus test on a cigarette filter water extract. Background technique [0002] The micronucleus test is a genotoxicity test that detects chromosomal or mitotic organ damage. Chromosome fragments without a centromere or entire chromosomes lost due to spindle damage remain in the cytoplasm of daughter cells during the anaphase of cell division and become micronuclei. The micronucleus test in vitro generally uses animal cells cultured in vitro to detect micronuclei. It is often used as one of the safety biological detection methods for food, medicine, dental materials, etc. [0003] Due to the direct contact between the cigarette filter and the human mouth, there are potential health risks. Therefore, it is necessary to use in vitro micronucleus test methods to investigate the potential hazards t...

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Problems solved by technology

[0005] However, the existing method is not suitable for the in vitro micronucleus test of the cigarette filter extract. This is because the cigarette filter extract needs to be prepared for the in vitro micronucleus test of the cigarette filter. Therefore, the above-mentioned first method is used to set Positive controls cannot verify the reliability of the extraction process

As for the use of the second method, since no genotoxicity has been found in the existing cigarette filter varieties, and there is no genotoxic material with similar properties to cigarette filters, in order to verify the extraction process of cigarette filters and follow-up Whether the in vitro micronucleus test performed on the water extract of cigarette filter is reliable, it is necessary to develop a positive control setting method for the external micronucleus test of the water extract of cigarette filter

[0006] Through literature search, do not find the public report of the same literature as the content of the present invention

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