Method for detecting horse-breed and mule-breed derived ingredients in gelatin-type traditional Chinese medicine and products

A technology of glue-like traditional Chinese medicine and detection method, which is applied in the direction of material separation, material analysis, measuring device, etc., to achieve the effect of high content and low degree of damage

Active Publication Date: 2015-01-14
SHAN DONG DONG E E JIAO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is currently no method for detecting the components of horse or mule origin in gelatinous traditional Chinese medicine and its products from the perspective of collagen peptides.

Method used

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  • Method for detecting horse-breed and mule-breed derived ingredients in gelatin-type traditional Chinese medicine and products
  • Method for detecting horse-breed and mule-breed derived ingredients in gelatin-type traditional Chinese medicine and products
  • Method for detecting horse-breed and mule-breed derived ingredients in gelatin-type traditional Chinese medicine and products

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The detection of horse species and mule provenance components in the colloid traditional Chinese medicine of embodiment 1

[0023] 1 Materials and Reagents

[0024] Materials: Pure glue includes horsehide glue, donkey mule hide glue, horse mule hide glue, donkey-hide gelatin, yellow gelatin, pigskin glue, tortoise shell glue, and deer antler glue. , Cowhide, Pigskin, Tortoise Shell, and Antlers are obtained by decocting.

[0025] Reagents: ammonium bicarbonate (analytical grade), trypsin (sequence pure, purchased from China Institute for the Control of Pharmaceutical and Biological Products), synthetic oligopeptide Gly-Ala-Ser-Gly-Pro-Ala-Gly-Val-Arg (Gill Biochemical Shanghai Co., Ltd).

[0026] 2 detection method

[0027] (1) Preparation of synthetic oligopeptide control sample enzymatic hydrolysis solution

[0028] Get 1.0g sample of pure donkey-hide gelatin to be tested in 500mL measuring bottle, add a small amount of mass fraction to be 1%NH 4 HCO 3 (pH8.0) s...

Embodiment 2

[0034] The detection of horse breed and mule provenance component in the colloid traditional Chinese medicine of embodiment 2

[0035] 1 Materials and Reagents

[0036] Materials: Pure glue includes horsehide glue, donkey mule hide glue, horse mule hide glue, donkey-hide gelatin, yellow gelatin, pigskin glue, tortoise shell glue, and deer antler glue. , Cowhide, Pigskin, Tortoise Shell, and Antlers are obtained by decocting.

[0037] Mixed gum samples (donkey-hide gelatin with a mass fraction of 5% horsehide glue, donkey-hide gelatin with a mass fraction of 10% horsehide glue, donkey-hide gelatin with a mass fraction of 20% horsehide glue, and donkey-hide gelatin with a mass fraction of 40% horsehide glue) The above-mentioned pure gum is precisely mixed.

[0038] Reagents: ammonium bicarbonate (analytical grade), trypsin (sequence pure, purchased from China Institute for the Control of Pharmaceutical and Biological Products), synthetic oligopeptide Gly-Ala-Ser-Gly-Pro-Ala-Gl...

Embodiment 3

[0047] Example 3 Detection of Horse Breed and Mule Progeny Components in Glue Traditional Chinese Medicine Added with Horsehide Glue

[0048] 1 Materials and Reagents

[0049] Materials: The mixed glue sample is made by adding 5% horsehide glue into the pure glue sample in Example 2, including donkey-hide gelatin with 5% horsehide glue and 5% horsehide glue. yellow gelatin, pigskin glue with 5% horse hide glue, tortoise shell glue with 5% horse hide glue in mass fraction, and deer antler glue with 5% horse hide glue in mass fraction.

[0050] Reagents: ammonium bicarbonate (analytical grade), trypsin (sequence pure, purchased from China Institute for the Control of Pharmaceutical and Biological Products), synthetic oligopeptide Gly-Ala-Ser-Gly-Pro-Ala-Gly-Val-Arg (Gill Biochemical Shanghai Co., Ltd).

[0051] 2 detection method

[0052] (1) Preparation of synthetic oligopeptide control sample enzymatic hydrolysis solution

[0053] Get 1.0g sample of pure donkey-hide gelati...

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Abstract

The invention relates to a method for detecting horse-breed and mule-breed derived ingredient in gelatin-type traditional Chinese medicine and products. On the basis that specific oligopeptides Gly-Ala-Ser-Gly-Pro-Ala-Gly-Val-Arg is contained in a horse-breed and mule-breed collagen sequence, the specific oligopeptides Gly-Ala-Ser-Gly-Pro-Ala-Gly-Val-Arg is free out from the collagen by virtue of enzyme digestion of restriction endonuclease, and then the gelatin-type traditional Chinese medicine and products are detected by adopting liquid-phase chromatography-tandem mass spectrometry. The method is strong in characteristic, high in sensitivity, simple in operation and capable of identifying whether the gelatin-type traditional Chinese medicine and products are mixed with the horse-breed and mule-breed derived ingredients.

Description

technical field [0001] The invention relates to a detection method for components derived from horse breeds and mules, in particular to a detection method for components derived from horse breeds and mules in gelatinous traditional Chinese medicines and products thereof, and belongs to the field of detection of traditional Chinese medicines. Background technique [0002] Glue-like traditional Chinese medicines are boiled from animal skins and bones to make glue-like substances, including donkey-hide gelatin, tortoise shell glue, antler glue, etc. Traditional Chinese medicine with typical ethnic characteristics is widely used in blood, gynecology, obstetrics, respiratory, tumor and other diseases prevention and treatment. [0003] With the continuous development of the glue-type traditional Chinese medicine market, the number of manufacturers producing glue-type traditional Chinese medicine is also increasing, and the production capacity and product quality are uneven. Takin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02C07K7/06
Inventor 尤金花周祥山秦玉峰田守生郭尚伟段小波嵇传良史兆松
Owner SHAN DONG DONG E E JIAO
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