A kind of method for rooting outside the bottle of hosta tissue culture seedlings

Abstract

The invention relates to a fragrant plantain lily tissue culture seedling vitro-bottle rooting method which comprises the following steps: (1) preparing a rooting agent; (2) preparing a transplant matrix; (3) carrying out bottled seedling selection and seedling hardening; (4) transplanting tissue culture seedlings; (5) managing after transplanting. According to the fragrant plantain lily tissue culture seedling vitro-bottle rooting method, an effective fragrant plantain lily tissue culture seedling vitro-bottle rooting technology is established, and a tissue culture rapid-propagation system is optimized. According to the technology, the tissue culture in-bottle rooting procedure is not needed, rooting and domestication are performed at the same time, the seedling raising period is shortened by more than one month, the rooting rate is greater than 95%, and the transplant survival rate is 100%; the vitro-bottle rooting technology is capable of reducing the production cost by about 40% and saving the tissue culture space by more than 50%, and has great significance in improving the fragrant plantain lily tissue culture industrialization level.

Description

Technical field [0001] The invention relates to a method for raising seedlings of hosta, in particular to a method for rapid rooting of hosta tissue culture seedlings outside the bottle. Background technique [0002] Hosta (Hosta), also known as Baihexian, Golden Pincao, and Small Banana, is a perennial perennial root herb of the Liliaceae family. Hosta is native to China, Korea and Japan, and is widely distributed in temperate and subtropical regions of East Asia. Hosta has a wide variety of flowers and leaves, and some flowers are scented, resistant to stress, and extremely shade-tolerant. They are widely used in garden landscaping and indoor viewing. By the end of the 20th century, they have become the world's first-selling herbal flower. In addition, the whole hosta plant can be human medicine, has the effects of clearing heat, detoxification, hemostasis, and relieving cough. It is also an important medicinal plant. At present, there are more than 4000 horticultural cultivar...

AI Technical Summary

Problems solved by technology

However, existing research and practice have shown that the rooting of hosta tissue culture seedlings in bottles has problems such as high cost, long cycle, and low transplanting survival rate.

[0004] In recent years, scholars at home and abroad have proposed the technology of rooting tissue culture seedlings outside the bottle, and there have been many reports on the rooting of tissue culture seedlings in China, involving Sapindus (Fujian Forestry, 2013, 6: 24-25), Wasabi (Jinggangshan University Journal of the Chinese Academy of Sciences, 2014, 1: 43-45), blueberry (Forestry Science and Technology, 2013, 5: 4-6), sugarcane (Anhui Agricultural Science, 2012, 29: 14191-14194), Radix Pseudostellariae (Journal of Liupanshui Normal University, 2013, 4:27-30), European and American poplar (Plant Physiology Communications, 2010, 10:1050-1054), Anthurium (Forestry Science and Technology, 2013, 5:7-9), Gerbera (Northern Horticulture, 2013, 9:78- 80), etc., but there is no report on the technology of rooting outside the hosta bottle