Method for promoting rooting of tissue culture seedlings of blueberry

A technology for tissue culture seedlings and black rice is applied to the field of rapid out-of-bottle rooting of tissue culture seedlings of Wufan tree, which can solve the problems of high cost, low transplanting survival rate, long period and the like, achieves developed root system, save tissue culture space, Easy to use effect

Inactive Publication Date: 2018-11-23
JIANGSU ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

However, the existing research and practice have shown that rooting in bottle of tissue culture seedlings of black rice tree has problems such as high cost, long cycle, and low survival rate of transplanting.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0021] Example 1: a method for promoting the rooting of black rice tree tissue culture seedlings, the method is carried out as follows:

[0022] (1) Selection of bottle seedlings: Select well-growing tissue-cultured seedlings of the black rice tree with a height of about 8-10 cm and strong branches for hardening and domestication.

[0023] (2) Seedling hardening: first place the tissue-cultured seedlings of the black rice tree in the hardening room. The hardening room is dominated by scattered light, the light intensity is 3000Lux, and the temperature is controlled at 25°C. The seedling room continued to be cultivated for 3 days.

[0024] (3) Cutting of tissue cultured seedlings outside the bottle: Take the seedlings out of the bottle, wash the medium sticking to the base of the plant, cut them off as a single plant, and remove the yellowed and damaged leaves at the bottom; cut each plant into 3-4 cm with buds For the stem section, dip in rooting water before cutting, the dep...

example 2

[0028] Example 2: a method for promoting rooting of black rice tree tissue culture seedlings, the method is carried out as follows:

[0029] (1) Prepare rooting agent:

[0030] The preparation method of every liter of rooting agent solution is as follows: 500 mg ABT rooting powder No. 1 is dissolved with a small amount of ethanol, and the volume is adjusted to 1 L with clear water;

[0031] (2) Preparation of transplanting substrate: put perlite into a plastic seedling tray and pour water thoroughly for later use; sterilize the substrate with 20g 50% carbendazim wettable powder per cubic meter;

[0032] (3) Selection of bottle seedlings: select well-growing tissue-cultured seedlings of black rice tree, about 8-10 cm in height, with strong branches, and carry out hardening and domestication;

[0033] (4) Seedling hardening: first place the tissue-cultured seedlings of the black rice tree in the hardening room, the hardening room is mainly based on scattered light, the light in...

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PUM

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Abstract

The invention relates to a method for promoting rooting of tissue culture seedlings of blueberry, which mainly solves the technical problems of multiple culture procedures, low survival rate, long period and the like in the rooting of tissue culture seedlings of the blueberry. The method comprises the following steps: (1) bottle seedling selection; (2) seedlings hardening; (3) tissue culture seedlings transplantation; and (4) post-transplantation management. An effective rooting technique of blueberry tissue culture seedling is established and the tissue culture and rapid propagation system isoptimized. The technique does not need to go through the rooting procedure in a tissue culture bottle, rooting and domestication are carried out at the same time, and a large amount of roots are produced in the seedling base after 25 to 30 days. A robust seedling base is obtained after 2 months. Compared with traditional bottle rooting, the seedling cycle is shortened by more than one month, andthe transplanting survival rate is more than 95%. Besides, the external rooting technology of the bottle can reduce the production cost by about 40% and save tissue culture space by more than 50%, which is of great significance for intensive production of the blueberry tissue culture.

Description

technical field [0001] The invention relates to a method for raising seedlings of the black rice tree, in particular to a method for rapid external rooting of the tissue cultured seedlings of the black rice tree. Background technique [0002] Black rice tree ( Vaccinium bracteatum ) is the genus Vaccinium (Ericacece) of Rhododendronaceae ( Vaccinium ) Evergreen shrubs or small trees, widely distributed in low-altitude mountains south of the Yangtze River, and their habitats are mostly mountain forest edges, sparse forests or shrubs. Black rice tree has a long history of application and broad development prospects in my country. The leaves can be soaked in glutinous rice for cooking, especially in Jiangsu and Zhejiang areas since ancient times. There has been a custom of eating "black rice"; the berries are sweet and sour after ripening, and can be used to make jam and wine; The plant body is rich in flavonoids, amino acids, vitamins, mineral elements and other components, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G2/10A01G17/00A01G7/06
CPCA01G7/06A01G17/005A01G2/10
Inventor 张敏周鹏陈庆生周洁黄婧
Owner JIANGSU ACAD OF FORESTRY
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