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Ex-vitro rooting method of Callicarpa cathayana tissue culture seedlings

A technology of tissue culture seedlings and external rooting, applied in horticultural methods, botany equipment and methods, plant regeneration, etc., can solve the problems of long cycle, unseen flower rooting technology in bottle, high cost, etc., and achieve easy operation , the shortening of seedling cultivation period and the effect of well-developed root system

Active Publication Date: 2020-02-14
JIANGSU ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, existing studies and practices have shown that the rooting of Huazizhu tissue culture seedlings in bottles has problems such as high cost and long cycle.
In recent years, scholars at home and abroad have proposed the technology of rooting tissue culture seedlings outside the bottle. There have been many domestic reports on the rooting of tissue culture seedlings outside the bottle, involving blueberry (Forestry Science and Technology, 2013, 5: 4-6), black rice tree (Jiangsu forestry Science and Technology, 2018, (2):29-33.), Palma (Seed, 2018, 37(11):141-145.), Australian Tea Tree (Western Forestry Science, 2018, 47(2):86-90 ), Hippophae rhamnoides (Economic Forest Research, 2018(3):182-186.), Blackwood Acacia (Journal of Forest and Environment, 2019, 39(1):21-26), etc., but there is no external rooting technology of Huazizhu report

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1: a kind of method of Huazizhu tissue culture seedling rooting outside the bottle, the method is carried out according to the following steps:

[0025] 1. a method for rooting outside the bottle of Huazizhu tissue culture seedling, is characterized in that, comprises the steps:

[0026] Configuration of WPM medium:

[0027] The components of the constant elements and their corresponding use concentrations are as follows: NH 4 NO 3 200 mg / L, Ca(NO 3 ) 2 4H 2 O 278 mg / L, CaCl 2 2H 2 O 48mg / L, MgSO 4 ·7H 2 O 185 mg / L, KH 2 PO 4 85 mg / L, K 2 SO 4 495mg / L;

[0028] The components of trace elements and their corresponding concentration are as follows: manganese sulfate (MnSO 4 ·H 2 O) 22.3 mg / L, zinc sulfate (ZnSO 4 ·7H 2 O) 8.6 mg / L, boric acid (H 3 BO 3 ) 6.2 mg / L, sodium molybdate (Na 2 MoO 4 2H 2 O) 0.25 mg / L, copper sulfate (CuSO 4· 5H 2 O) 0.025 mg / L, cobalt chloride (CoCl 2 .6H 2 O) 0.025 mg / L;

[0029] The components of iron salt...

Embodiment 2

[0042] Embodiment 2: a kind of method of taking root outside the bottle of Huazizhu tissue culture seedling, the method is carried out according to the following steps:

[0043] Configuration of WPM medium:

[0044] The components of the constant elements and their corresponding use concentrations are as follows: NH 4 NO 3 200 mg / L, Ca(NO 3 ) 2 4H 2 O 278 mg / L, CaCl 2 2H 2 O 48mg / L, MgSO 4 ·7H 2 O 185 mg / L, KH 2 PO 4 85 mg / L, K 2 SO 4 495mg / L;

[0045] The components of trace elements and their corresponding concentration are as follows: manganese sulfate (MnSO 4 ·H 2 O) 22.3 mg / L, zinc sulfate (ZnSO 4 ·7H 2 O) 8.6 mg / L, boric acid (H 3 BO 3 ) 6.2 mg / L, sodium molybdate (Na 2 MoO 4 2H 2 O) 0.25 mg / L, copper sulfate (CuSO 4· 5H 2 O) 0.025 mg / L, cobalt chloride (CoCl 2 .6H 2 O) 0.025 mg / L;

[0046] The components of iron salts and their corresponding usage concentrations are as follows: disodium edetate (Na 2 EDTA) 37.3 mg / L, ferrous sulfate (FeSO 4 ·...

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Abstract

The invention relates to an ex-vitro rooting method of Callicarpa cathayana tissue culture seedlings. The method includes the steps of firstly, sterilizing plant tissue; secondly, culturing tissue culture seedlings; thirdly, performing ex-vitro tissue culture seedling cuttage; fourthly, performing management after cuttage. The method has the advantages that the effective ex-vitro rooting technology of the Callicarpa cathayana tissue culture seedlings is built, the technology does not need the tissue culture in-vitro rooting procedure, rooting and acclimation are performed at the same time, theseedlings can growth a large number of root systems in 25-30 days, and robust rooted seedlings can be obtained in 2 moths; the method is short in seedling culture cycle, seedling rooting rate is larger than 90%, the seedling transplanting survival rate is larger than 95%, production cost can be lowered, tissue culture space can be saved, and the method is of important significance to the improving of the industrial Callicarpa cathayana tissue culture level.

Description

technical field [0001] The invention relates to a method for raising seedlings of huazizhu, in particular to a method for rooting out of a bottle of huazizhu tissue culture seedlings. Background technique [0002] Purple Pearl ( Callicarpa L.) is a deciduous shrub belonging to Verbenaceae and Zizhu. It has beautiful plant type, luxuriant branches and leaves, rich flower colors, bright fruit color, and round and jade-like pearls. It can be used as a fruit-watching tree species in autumn for foundation planting, lawn or forest edge lining; It can also be used as potted ornamental and cut flower materials. Most of the plants in this genus have the effects of antibacterial and hemostatic, detoxification and anti-inflammatory, wind-dispelling and damp-dispelling, and anti-tumor. Both leaves and roots can be used as medicine. [0003] Hua Zizhu ( C. cathayana ) is a plant of the Verbenaceae purple pearl genus. It is a unique plant in China. It grows in areas with an altitude ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 黄婧陈庆生张敏周鹏
Owner JIANGSU ACAD OF FORESTRY
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