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Method for promoting rooting of ilex verticillata tissue cultured seedlings

A technology for tissue culture seedlings and holly, which is applied in the field of rapid out-of-bottle rooting of North American holly tissue culture seedlings, which can solve the problems of low transplanting survival rate, long seedling cultivation cycle, and high production cost, and achieve improved cultivation space and good seedling consistency , the effect of easy operation

Inactive Publication Date: 2020-02-14
JIANGSU ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the rooting of North American holly test-tube seedlings still adopts the traditional bottle rooting, which has high production costs, long seedling cultivation period (60 days for rooting in the bottle and transplanting), and low transplanting survival rate (the root system of the rooting seedlings in the tissue culture bottle Weak and weak, the survival rate of transplanting is less than 70%) and other problems

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1: a kind of method that North American Ilex tissue culture micro-cuttings directly takes root, the method is carried out as follows:

[0024] (1) Selection of bottle seedlings: select well-growing North American holly tissue culture seedlings, with a height of about 8-10 cm, and strong branches, for hardening and domestication;

[0025] (2) Seedling hardening: First place the North American holly tissue culture seedlings in the hardening room. The hardening room is dominated by scattered light, the light intensity is 4000Lux, and the temperature is controlled at 15°C. room to continue culturing for 2 days;

[0026] (3) Cutting of tissue cultured seedlings outside the bottle: Take the seedlings out of the bottle, wash the medium sticking to the base of the plant, cut them off as a single plant, and remove the yellowed and damaged leaves at the bottom; cut each plant into 3-4 cm with buds For the stem section, dip the rooting water before cutting. The rooting...

Embodiment 2

[0028] Embodiment 2: a kind of method that North American Ilex tissue culture micro-cuttings directly takes root, the method is carried out as follows:

[0029] (1) Prepare rooting agent:

[0030] The preparation method of every liter of rooting agent solution is as follows: 500 mg ABT rooting powder No. 1 is dissolved with a small amount of ethanol, and the volume is adjusted to 1 L with clear water;

[0031] (2) Transplanting substrate preparation: put peat into plastic seedling trays, water thoroughly for later use; substrate is sterilized with 20 g 50% carbendazim wettable powder per cubic meter;

[0032] (3) Selection of bottle seedlings: select well-growing North American holly tissue culture seedlings, with a height of about 8-10 cm, and strong branches, for hardening and domestication;

[0033] (4) Seedling hardening: first place the North American holly tissue culture seedlings in the hardening room. The hardening room is dominated by scattered light, the light inten...

Embodiment 3

[0036] Embodiment 3: a kind of method that North American Ilex tissue culture micro-cuttings directly takes root, the method is carried out as follows:

[0037] (1) Selection of bottle seedlings: select well-growing North American holly tissue culture seedlings, with a height of about 8-10 cm, and strong branches, for hardening and domestication;

[0038] (2) Seedling hardening: First place the North American holly tissue culture seedlings in the hardening room. The hardening room is dominated by scattered light, the light intensity is 3500Lux, and the temperature is controlled at 20°C. room to continue culturing for 2 days;

[0039](3) Cutting of tissue cultured seedlings outside the bottle: Take the seedlings out of the bottle, wash the medium sticking to the base of the plant, cut them off as a single plant, and remove the yellowed and damaged leaves at the bottom; cut each plant into 3-4 cm with buds For the stem section, dip in rooting water before cutting. The rooting w...

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Abstract

The invention relates to a method for promoting rooting of ilex verticillata tissue cultured seedlings and mainly solves technical problems of excessive culture procedures, low survival rate, long period and the like in rooting of existing ilex verticillata tissue cultured seedlings. The method includes steps: (1) bottle seedling selection; (2) hardening; (3) ex-vitro cuttage of the tissue cultured seedlings; (4) after-cuttage management. An effective ex-vitro rooting technique for the ilex verticillata tissue cultured seedlings is created, and a tissue-culture rapid propagation system is optimized. The technique avoids an in-vitro rooting procedure, rooting and domestication are carried out at the same time, base parts of the seedlings grow a great quantity of root systems in 25-30d, andstrong rooted seedlings can be obtained in 2 months. Compared with traditional in-vitro rooting, the method has advantages that a seedling cultivation period is shortened by 1 month or more, the rooting rate is greater than 93%, the transplanting survival rate is greater than 95%, production cost can be reduced by about 40%, the tissue culture space is saved by 50% or more, and the method is significant to tissue culture intensive production of ilex verticillata.

Description

technical field [0001] The invention relates to a method for raising seedlings of North American holly, in particular to a method for rapid out-of-bottle rooting of North American holly tissue culture seedlings. Background technique [0002] North American holly ( Ilex verticillata ) is a plant of the Ilex family Ilex. After the leaves fall in winter, the red fruits on the branches are more festive and gorgeous. It is a famous cutting tree species, and the market name is "red bean". The market time of red beans is from the end of November to around New Year's Day every year. It can very well set off the festive atmosphere of New Year's Day, Christmas, Lantern Festival, Valentine's Day, etc., and its market recognition in our country is very high. At present, the production of cut branches of North American holly in China is still in the initial stage, and my country needs to import a large amount of them every year. [0003] Scholars at home and abroad have carried out a l...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G2/10A01H4/00
CPCA01H4/008A01G2/10
Inventor 陈庆生张敏周鹏黄婧宋倩云
Owner JIANGSU ACAD OF FORESTRY
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