A kind of outside rooting method of Hua Zizhu tissue culture seedling in bottle

A technology of tissue culture seedlings and external rooting, applied in horticultural methods, botany equipment and methods, plant regeneration, etc., can solve the problems of long cycle, high cost, and no external rooting technology of Huazizhu bottle, and achieve easy operation , developed root system and shortened seedling cycle

Active Publication Date: 2022-07-15
JIANGSU ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, existing studies and practices have shown that the rooting of Huazizhu tissue culture seedlings in bottles has problems such as high cost and long cycle.
In recent years, scholars at home and abroad have proposed the technology of rooting tissue culture seedlings outside the bottle. There have been many domestic reports on the rooting of tissue culture seedlings outside the bottle, involving blueberry (Forestry Science and Technology, 2013, 5: 4-6), black rice tree (Jiangsu forestry Science and Technology, 2018, (2):29-33.), Palma (Seed, 2018, 37(11):141-145.), Australian Tea Tree (Western Forestry Science, 2018, 47(2):86-90 ), Hippophae rhamnoides (Economic Forest Research, 2018(3):182-186.), Blackwood Acacia (Journal of Forest and Environment, 2019, 39(1):21-26), etc., but there is no external rooting technology of Huazizhu report

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1: a kind of method for rooting outside the bottle of Hua Zizhu tissue culture seedling, the method is carried out according to the following steps:

[0025] 1. a method for rooting outside a tissue culture seedling bottle of Hua Zizhu, is characterized in that, comprises the steps:

[0026] Configuration of WPM medium:

[0027] The composition of the major elements and their corresponding use concentrations are as follows: NH 4 NO 3 200 mg / L, Ca(NO 3 ) 2 ·4H 2 O 278mg / L, CaCl 2 ·2H 2 O 48mg / L, MgSO 4 ·7H 2 O 185 mg / L, KH 2 PO 4 85 mg / L, K 2 SO 4 495 mg / L;

[0028] The components of trace elements and their corresponding concentrations are as follows: manganese sulfate (MnSO 4 ·H 2 O) 22.3 mg / L, zinc sulfate (ZnSO 4 ·7H 2 O) 8.6 mg / L, boric acid (H 3 BO 3 ) 6.2 mg / L, sodium molybdate (Na 2 MoO 4 ·2H 2 O) 0.25 mg / L, copper sulfate (CuSO 4· 5H 2 O) 0.025 mg / L, cobalt chloride (CoCl 2 .6H 2 O) 0.025 mg / L;

[0029] The components of iro...

Embodiment 2

[0042] Embodiment 2: a method for rooting out of a tissue culture seedling bottle of Hua Zizhu, the method is carried out according to the following steps:

[0043] Configuration of WPM medium:

[0044] The composition of the major elements and their corresponding use concentrations are as follows: NH 4 NO 3 200 mg / L, Ca(NO 3 ) 2 ·4H 2 O 278mg / L, CaCl 2 ·2H 2 O 48mg / L, MgSO 4 ·7H 2 O 185 mg / L, KH 2 PO 4 85 mg / L, K 2 SO 4 495 mg / L;

[0045] The components of trace elements and their corresponding concentrations are as follows: manganese sulfate (MnSO 4 ·H 2 O) 22.3 mg / L, zinc sulfate (ZnSO 4 ·7H 2 O) 8.6 mg / L, boric acid (H 3 BO 3 ) 6.2 mg / L, sodium molybdate (Na 2 MoO 4 ·2H 2 O) 0.25 mg / L, copper sulfate (CuSO 4· 5H 2 O) 0.025 mg / L, cobalt chloride (CoCl 2 .6H 2 O) 0.025 mg / L;

[0046] The components of iron salts and their corresponding concentrations are as follows: disodium EDTA (Na 2 ·EDTA) 37.3 mg / L, ferrous sulfate (FeSO 4 ·7H 2 O) 27.8 mg / L;...

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PUM

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Abstract

The invention relates to a method for rooting out of a bottle of tissue culture seedlings of Hua Zizhu, comprising the following steps: (1) disinfection of plant tissues; (2) culture of tissue culture seedlings; (3) cuttings of tissue culture seedlings outside the bottle; (4) cuttings post management. The invention establishes an effective rooting technology outside the bottle for tissue culture seedlings of Hua Zizhu. The technology does not need to go through the rooting procedure in the tissue culture bottle. Robust rooting shoots. The invention has short seedling raising period, rooting rate greater than 90%, transplanting survival rate greater than 95%, can reduce production cost and save tissue culture space, and is of great significance for improving the level of tissue culture industrialization of Hua Zizhu.

Description

technical field [0001] The invention relates to a method for raising seedlings of Radix chinensis, in particular to a method for rooting outside a bottle of tissue culture seedlings of Radix chinensis. Background technique [0002] Purple Pearl ( Callicarpa L.) is a deciduous shrub of the Verbena family and the genus Viola, with beautiful plant shape, lush branches and leaves, rich flowers, bright fruit colors, and round and jade-like beads. It can be used as a fruit tree species in autumn for foundation planting, lawn or forest edge lining; It can also be used as potted ornamental and cut flower material. Most of the plants in this genus have the functions of antibacterial and hemostasis, detoxification and anti-inflammatory, expelling wind and dampness and anti-tumor. Both leaves and roots can be used as medicines. [0003] Hua Zizhu ( C. cathayana ) is a plant of the genus Verbena, endemic to China, growing at an altitude of 1,200 meters, mostly in valleys, hillsides...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 黄婧陈庆生张敏周鹏
Owner JIANGSU ACAD OF FORESTRY
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