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Tissue culture and rapid propagation method for eucalyptus urophydis DH32-29 varieties

A technology of tissue culture rapid propagation and varieties, applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve the problems of ineffective inheritance of good characteristics of parents, ineffective development of tissue culture work, and outstanding characteristics to be studied. Achieve the effects of improving economic and social benefits, being easy to promote and use, and reducing the risk of tissue culture

Active Publication Date: 2015-01-28
广西八桂种苗高科技集团股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The above-mentioned patent uses the stem section of Eucalyptus urophylla aseptic seedlings as explants to induce embryogenic callus, and then obtains regenerated plants through the regeneration of somatic embryogenesis. The operation is relatively complicated and difficult to implement. The stems of the seedlings are explants, and the excellent characteristics of the female parent need to be studied. The commercial seedling cultivation using this technology alone cannot effectively inherit the excellent characteristics of the parents.
[0009] It can be seen that although there are many studies on the tissue culture technology of eucalyptus at present, there is still a lot of work to be explored for the purposeful propagation of the excellent traits of the excellent trees, and the tissue culture rapid propagation method that can be effectively popularized and applied. Due to the DH32-29 It is a perennial species of Eucalyptus urophylla. There are few tissue-cultured seedlings in the market and resources are in short supply. However, the tissue culture of this variety has not been effectively carried out. The tissue culture research on the new variety of Eucalyptus urophylla DH32-29 is helpful for the rapid development of this fine variety. It is of great significance to breed, meet the market supply, and improve the ecological and economic benefits of this fine variety

Method used

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  • Tissue culture and rapid propagation method for eucalyptus urophydis DH32-29 varieties

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Embodiment 1

[0032] The tissue culture rapid propagation method of Eucalyptus macrocephalus DH32-29 kind, concrete steps are as follows:

[0033] ①Establishment of explants: Select 4-5 years old trees with strong growth, thick trunks, and no pests and diseases, and cut them down from a place 20-25cm above the ground. When sprouting, the bud strips were taken back as explant material. In order to reduce the source of material pollution, when taking bud strips, it should be carried out after more than three consecutive days of fine weather. After removing most of the leaves from the harvested bud strips, rinse them with tap water for 15 minutes, soak them in 0.5% washing powder solution for 15 minutes, and then rinse them with tap water.

[0034] ② Disinfection of explants: step ① Soak the obtained explants in 75% alcohol for 30 seconds, rinse them with sterile water for 5 times, drain the water, put them in 0.1% mercuric chloride solution for disinfection for 9 minutes, and rinse them with...

Embodiment 2

[0041] The tissue culture rapid propagation method of Eucalyptus macrocephalus DH32-29 kind, concrete steps are as follows:

[0042] ①Establishment of explants: Select 4-5 years old trees with strong growth, thick trunks, and no pests and diseases, and cut them down from a place 20-25cm above the ground. When sprouting, the bud strips were taken back as explant material. In order to reduce the source of material pollution, when taking bud strips, it should be carried out after more than three consecutive days of fine weather. After removing most of the leaves from the harvested bud strips, rinse them with tap water for 15 minutes, soak them in 0.5% washing powder solution for 15 minutes, and then rinse them with tap water.

[0043] ② Disinfection of explants: step ① Soak the obtained explants in 75% alcohol for 30 seconds, rinse them with sterile water for 4 times, drain the water, put them in 0.1% mercuric chloride solution for 9 minutes, and rinse them with sterile water fo...

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Abstract

The invention discloses a tissue culture and rapid propagation method for eucalyptus urophydis DH32-29 varieties. The tissue culture and rapid propagation method is realized through the steps of explant establishing, explant disinfection, primary culture, subculture, rooting culture, acclimatization, transplanting and the like; a special culture medium is selected for primary culture, subculture and rooting culture, and reasonable acclimatization measures are adopted, so that the transplanted eucalyptus urophydis is rapid in growth and high in survival rate, and the aims of high propagation coefficient and good propagation effect are achieved. Meanwhile, the method disclosed by the invention is low in production cost, is an effective path for tissue culture and rapid propagation of a eucalyptus and is wide in application range; and the genetic characters of the obtained tissue culture seedlings are consistent.

Description

technical field [0001] The invention relates to the technical field of eucalyptus cultivation, in particular to a method for tissue culture and rapid propagation of Eucalyptus macrocephalus DH32-29 variety. Background technique [0002] Eucalyptus (Eucalyptus) is the general name of Myrtaceae (Myrtaceae) Eucalyptus (Eucalyptus) plants. It is native to Australia. There are more than 100 species, of which more than 40 are widely cultivated. In recent years, all parts of our country are vigorously developing eucalyptus planting, forming a certain scale in Guangdong, Guangxi, Hainan, Sichuan, Yunnan and other provinces, which has produced obvious ecological and economic benefits. [0003] Eucalyptus grandis DH32-29 variety ( E. urophylla × E. Grandis) , is Guangxi Dongmen Forest Farm, with Indonesian provenance Eucalyptus urophylla U16 ( Eucalyptus urophylla ) as the female parent, and the Australian provenance Eucalyptus grandis G46 ( Eucalyptus grandis ) is an excellent ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCY02A40/22Y02P60/40
Inventor 庞贞武沈云黄全东吴兵梁秀莉陈允椿韦炳俭吴满芬俸荣娣邓冬丽付淼吕华丽莫继有兰俊唐再生吴永富龚卫新
Owner 广西八桂种苗高科技集团股份有限公司
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