Anti-bifenthrin monoclonal antibody and application thereof

A monoclonal antibody and phenthrin technology, applied in the field of biology, can solve the problems of fast and simple detection of a large number of samples, cumbersome sample pretreatment, time-consuming and laborious detection, etc., and achieve high accuracy, high sensitivity, and strong specificity Effect

Inactive Publication Date: 2015-02-04
无锡市白蚁防治中心 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These instrumental analysis methods are sensitive and accurate, but the detection instruments are expensive, the sample pretreatment is cumbersome, the detection is time-consuming and laborious, and it is difficult to meet the needs of rapid and simple detection of a large number of samples.

Method used

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  • Anti-bifenthrin monoclonal antibody and application thereof
  • Anti-bifenthrin monoclonal antibody and application thereof
  • Anti-bifenthrin monoclonal antibody and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0019] Example 1: Preparation of anti-bifenthrin monoclonal antibody

[0020] 1. Immunization of mice:

[0021] (1) Prepare the immunogen by coupling the immune hapten represented by formula (I) to BSA (bovine serum albumin);

[0022]

[0023] (2) Initial immunization: take healthy BALB / c female mice aged 6-8 weeks, prepare the prepared immunogen with PBS buffer to 1 mg / mL, mix it with an equal volume of Freund's complete adjuvant (FCA), and fully After emulsification, each mouse was intraperitoneally injected with 200 μL (i.e. 100 μg of immunogen);

[0024] (3) Booster immunization: three weeks after the first immunization, mix the immunogen (equal dose with the first immunization) and an equal volume of Freund's incomplete adjuvant (FIA), fully emulsify, and inject 200 μL intraperitoneally into each mouse Afterwards, booster immunization once every two weeks, a total of 4 immunizations; 3 days before cell fusion, the mice were injected with the immunogen, and the inject...

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PUM

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Abstract

The invention relates to an anti-bifenthrin monoclonal antibody and an application thereof and especially provides a monoclonal antibody capable of specifically recognizing bifenthrin, a hybrid tumor generating the monoclonal antibody and the application of the monoclonal antibody. The invention belongs to the field of biology. A method includes following steps: immunizing BALB / c female mice by an artificial antigen; performing cell fusion with myeloma cell SP2 / 0 and mice spleen cells to prepare a hybridoma; obtaining a hybridoma strain which can stably secret the anti-bifenthrin monoclonal antibody; injecting the hybridoma strain into abdomen of the mice to prepare ascites; purifying the ascites in an octanoic acid-ammonium sulfate manner to obtain the specific anti-bifenthrin specifically monoclonal antibody. The monoclonal antibody does not cross-react with other compounds. ELISA method established by the antibody can be used for quickly, sensitively, conveniently and cheaply detecting residual bifenthrin in environment and agricultural products.

Description

technical field [0001] The present invention relates to an anti-bifenthrin monoclonal antibody, especially a monoclonal antibody that can specifically recognize bifenthrin, a hybridoma producing the monoclonal antibody, and the use of the monoclonal antibody, belonging to The field of biological technology. Background technique [0002] Pyrethroids (Pyrethroids) insecticides are a class of ultra-high-efficiency insecticides imitated according to the chemical structure of natural pyrethroids. It has been used more and more widely in the past 30 years. Pyrethroid insecticides are mainly used to kill pests on cotton, vegetables, fruit trees, tea and other crops, and are broad-spectrum and highly effective insecticides. The chemical name of bifenthrin ester is (1R, S)-cis-(Z)-3-(2-chloro-3,3,3-trifluoro-1-propenyl)-2,2-dimethyl - Cyclopropanecarboxylic acid-2-methyl-3-phenylbenzyl ester, bifenthrin (Bifenthrin), as a strong termite repellent agent, has dual effects of prevent...

Claims

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Application Information

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IPC IPC(8): C07K16/44C12N5/20G01N33/577
Inventor 陆艺文王鸣华夏亚中华修德周秋君李伟
Owner 无锡市白蚁防治中心
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