Antigen polypeptide for detecting autoantibody of plasma immune marker VEGFR1 and application of antigen polypeptide

An antigen polypeptide and autoantibody technology, applied in the field of immunology, can solve the problems of low-standard qualitative detection, complex protein space structure, and high cost of recombinant protein, so as to prevent recurrence and metastasis, improve immune surveillance function, and prevent tumor formation and the effect of development

Active Publication Date: 2015-02-18
QINGDAO HAILANSHEN BIOTECH
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Problems solved by technology

At present, the only method in the field of biotechnology is to use the recombinant protein as the antigen to detect and screen the corresponding antibody of VEGFR1. , the linear epitope is not easily exposed, so the specificity of the antibody detected by the recombinant protein is poor, and a considerable proportion cannot bind to the antigen target on the surface of the tumor cell membrane. Practical means to prevent and treat tumors
At the same time, the high sensitivity of the ELISA method requires extremely high stability of the protein purification technology, so the recombinant protein that meets the detection requirements is expensive
At home and abroad, there is still no effective method for the detection and screening of VEGFR1 corresponding antibodies

Method used

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  • Antigen polypeptide for detecting autoantibody of plasma immune marker VEGFR1 and application of antigen polypeptide
  • Antigen polypeptide for detecting autoantibody of plasma immune marker VEGFR1 and application of antigen polypeptide
  • Antigen polypeptide for detecting autoantibody of plasma immune marker VEGFR1 and application of antigen polypeptide

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Embodiment Construction

[0025] 1. Sample collection: 121 normal adult plasma samples were collected, including 65 males and 56 females. The blood sample providers are all individuals who have not suffered from any type of tumor as determined by clinical physical examination. All plasma samples were stored at minus 80 degrees (-80°C) before operation, and it was verified that the storage time did not exceed two years, and there were no plasma samples that had been repeatedly frozen and thawed (no more than 3 times).

[0026] 2. Sample detection: Thaw plasma samples at 4°C. The three antigenic peptides listed in Table 1 used in this experiment were synthesized by SEVERN BIOTECH Co., Ltd., UK, with a purity of 95%. The specific steps are as follows:

[0027] (1) Before operation, each antigen polypeptide was dissolved in 67% acetic acid into a 5.7mg / ml stock solution, then mixed in equal volumes and stored in a -20°C refrigerator;

[0028](2) At the beginning of the operation, first dilute the mixture ...

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Abstract

The invention provides a VEGFR1 antigen polypeptide, which comprises three polypetides: H-DEGVYHCKATNQKGSVESSAYLTVQGTSDKSNLE-OH, H-DLKLSCTVNKFLYRDVTWILLRTVNNRTMHYSI-OH and H-ESGLSDVSRPSFCHSSCGHVSEGKRRFTYDHAEL-OH, and application of the antigen polypeptide for detecting the autoantibody of a plasma immune marker VEGFR1. The invention further provides a method of utilizing the VEGFR1 antigen polypeptide to detecting the autoantibody of the plasma immune marker VEGFR1, and a detecting kit containing the VEGFR1 antigen polypeptide.

Description

technical field [0001] The invention belongs to the technical field of immunology and relates to a VEGFR1 antigen polypeptide. The specific reagent for detecting VEGFR1 antibody level in human plasma can be prepared by using the antigen polypeptide. Background technique [0002] Studies in recent years have shown that the discovery and determination of tumor-associated antibodies in the blood of healthy people has the value of guiding targeted therapy. For example, trastuzumab and bevacizumab, which have been widely used in clinical practice, are labeled with epithelial cell growth factor type 2 receptor (HER2) and vascular endothelial cell growth factor A (VEGF-A). It has become an important means of clinical treatment of advanced tumors. However, due to too many side effects, these two monoclonal antibodies can only be used as third-line treatment drugs. Therefore, it is an urgent problem to develop new methods that can be used to prevent tumor metastasis and recurrence...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/71G01N33/68
CPCC07K14/71G01N33/6854G01N33/564G01N2333/71G01N33/574A61K39/001109A61K39/385G01N33/53A61K39/001103C07K14/705
Inventor 尉军王伟力
Owner QINGDAO HAILANSHEN BIOTECH
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