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Method for detecting glucose by paper-based colorimetric assay

A glucose and colorimetric technology, which is used in material analysis by observing the influence of chemical indicators, and analysis by chemical reaction of materials, etc., can solve the problems of expensive equipment, complex analysis process, long measurement cycle, etc. The effect of reducing experimental cost, simple post-processing, and simple operation

Inactive Publication Date: 2015-02-25
UNIV OF JINAN
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  • Summary
  • Abstract
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  • Application Information

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Problems solved by technology

Although these methods have different advantages, there are still deficiencies. For example, the above method requires specific equipment for the detection of glucose concentration, and the equipment itself is relatively expensive and not economical; universality
In addition, the above-mentioned method has a long measurement cycle, and the analysis process is relatively complicated, so it is impossible to realize the real-time measurement of glucose.

Method used

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  • Method for detecting glucose by paper-based colorimetric assay
  • Method for detecting glucose by paper-based colorimetric assay
  • Method for detecting glucose by paper-based colorimetric assay

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Experimental program
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Embodiment 1

[0033] Embodiment 1 (glucose in water)

[0034] A paper-based colorimetric method for detecting glucose with simple operation, fast detection speed and low cost, comprising the following steps:

[0035] 1. Self-made colorimeter, the schematic diagram is as follows figure 1 shown.

[0036] 2. Use Adobe Illustrator CS4 software to design hydrophobic wax printing pattern on the computer, the style is as follows figure 2 shown.

[0037] 3. Cut the filter paper into A4 size.

[0038] 4. Put the A4 filter paper cut in step 3 into the wax jet printer, and print the hydrophobic wax printing pattern designed in step 2 onto the A4 filter paper in step 3.

[0039] 5. Place the A4 filter paper with hydrophobic wax patterns obtained in step 4 on a heating plate and heat at 90°C for 1.5 min to melt the wax and soak through the entire thickness of the paper to form a hydrophobic wall.

[0040] 6. Preparation of ZnFe by the method reported in the literature 2 o 4 .

[0041] 7. Prepar...

Embodiment 2

[0050] Example 2 (glucose in serum)

[0051] A paper-based colorimetric method for detecting glucose with simple operation, fast detection speed and low cost, comprising the following steps:

[0052] 1. Self-made colorimeter, the schematic diagram is as follows figure 1 shown.

[0053] 2. Use Adobe Photoshop CS6 software to design hydrophobic wax printing pattern on the computer, the style is as follows figure 2 shown.

[0054] 3. Cut the filter paper into A4 size.

[0055] 4. Put the A4 filter paper cut in step 3 into the wax jet printer, and print the hydrophobic wax printing pattern designed in step 2 onto the A4 filter paper in step 3.

[0056] 5. Put the A4 filter paper with hydrophobic wax patterns obtained in step 4 into an oven and heat at 150°C for 0.5 min to melt the wax and soak through the entire thickness of the paper to form a hydrophobic wall.

[0057]6. Preparation of ZnFe by the method reported in the literature 2 o 4 .

[0058] 7. Prepare the mixed s...

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Abstract

The invention discloses a method for detecting glucose by paper-based colorimetric assay. The method comprises the following steps: manufacturing a homemade colorimeter; designing a hydrophobic wax printing pattern; cutting a piece of filter paper; printing the hydrophobic wax printing pattern onto the filter paper; molding by dewaxing; preparing ZnFe2O4; preparing a mixed solution; dropwise adding the mixed solution into a detection pool; dropwise adding a solution to be tested; folding the paper in half; and detecting by the homemade colorimeter. The method for detecting the glucose is simple to operate, can be operated by unprofessional people, and is high in detection speed, low in cost, simple in aftertreatment and free from pollution.

Description

technical field [0001] The invention relates to a paper-based colorimetric detection method for glucose, which belongs to the technical field of glucose detection. Background technique [0002] As the main component of blood sugar in the human body, glucose provides the main energy source for human activities, and its level directly affects people's physical condition. Among them, the harm of high blood sugar to human health is more and more serious, such as obesity, diabetes and other diseases. At present, diabetes has become the third major disease threatening human health after cancer and cardiovascular disease. According to the survey results of relevant international organizations in 2010, the number of diabetic patients in the world has reached 285 million, and the number of diabetic patients is still on the rise. At the same time, diabetes will also produce a variety of complications, such as cerebral infarction, cerebral hemorrhage, high blood pressure, etc., which...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78
Inventor 于京华张彦李念强张丽娜葛慎光颜梅刘海云黄加栋孙国强吴开庆吴鲁丹苏敏
Owner UNIV OF JINAN
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