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A method for improving the microspore embryogenesis rate of Utah

A microspore and Wutaicai technology, which is applied in the field of plant microspore embryo cultivation, can solve the problems of low embryo genotype ratio and low embryogenesis rate, achieve the effect of improving plant regeneration rate and solving technical obstacles

Active Publication Date: 2016-08-31
ANHUI AGRICULTURAL UNIVERSITY
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Problems solved by technology

[0003] The purpose of the present invention is to overcome the deficiencies in the prior art, and provides a method for improving the microspore embryogenesis rate of Utah cabbage, so as to solve the technical problems of low microspore embryogenesis rate and low embryo genotype ratio of Utah cabbage

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  • A method for improving the microspore embryogenesis rate of Utah
  • A method for improving the microspore embryogenesis rate of Utah
  • A method for improving the microspore embryogenesis rate of Utah

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Embodiment 1

[0021] 1. Experimental materials:

[0022] 1), B5 medium, purchased from Haibo Biotechnology Co., Ltd., Qingdao Hi-Tech Park, product number is HB8487-1.

[0023] 2), B5 extract, purchased from Qingdao Hi-Tech Park Haibo Biotechnology Co., Ltd., the product number is HB8487-2.

[0024] 3), the preparation of 1 / 2NLN-13:

[0025] Purchase NLN medium powder from Haibo Biotechnology Co., Ltd. in Qingdao Hi-Tech Park, product number HB8514, and prepare it into liquid NLN according to the requirements of the NLN medium powder specification sheet, add 13g sucrose in every 100ml liquid NLN, obtain NLN-13, will Mix and dilute NLN-13 and water at a volume ratio of 1:1 to obtain 1 / 2 NLN-13 with halved macroelements.

[0026] 2. Specific steps:

[0027] (1) Materials: 3 varieties of Wutaicai were taken, namely tadiwu 1, a variety with easy embryo emergence, tadiwu 6, a variety with weak embryo emergence, and tadiwu 2, a variety with difficult embryo emergence, and they were sown in Sep...

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Abstract

The invention discloses a method for improving microspore embryogenic rate of wuta-tsai. The method comprises the following steps: carrying out mixed culture to buds easy in embryogenesis and buds difficult or weak in embryogenesis, extracting by virtue of a B5 extracting solution, and inducing embryogenesis in a 1 / 2NLN-13 culture medium. Compared with the single culture of the prior art, the method disclosed by the invention can be used for effectively inducing the embryogenic rate of the variety difficult in embryogenesis and for improving the embryogenesis rate and the plant regeneration rate of the variety weak in embryogenesis, and the technical problem that the variety weak in embryogenesis is low in embryogenesis rate and embryogenesis genotypic ratio can be solved; meanwhile, the technical obstacle of generating plants from the variety difficult in embryogenesis by virtue of microspore culture is solved; and the method disclosed by the invention is significant in the microspore culture of the wuta-tsai.

Description

technical field [0001] The invention relates to a method for cultivating plant microspore embryos, in particular to a method for increasing the occurrence rate of microspore embryos of Utah cabbage. Background technique [0002] At present, microspore culture technology has been applied to Brassica vegetables such as Chinese cabbage, cabbage, and small green cabbage. However, there are few reports on the culture of free microspores of Utah. Due to differences in genotypes, it cannot The free microspore culture technology of Brassica vegetables such as Chinese cabbage was copied and applied to Wucai. However, in the rare reports on the culture of free microspores of Utakia, there are problems such as low embryonic genotype ratio and low embryo production rate. So far, there is no system that is completely suitable for microspores of Utah. cultivation method. Chinese patent document 201210047243.1 provides a culture method for increasing the embryonic birth rate of cabbage, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 汪承刚邹明倩朱世东袁凌云赵冠艳程圆刘姗葛继涛管超
Owner ANHUI AGRICULTURAL UNIVERSITY
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