Method for preparing guaijaverin and avicularin from guava leaf and use of method

Abstract

The invention provides a method for simultaneously preparing guaijaverin and avicularin from guava leaf and use of the method. The method provided by the invention comprises the following steps: crushing guava leaf as the raw material, extracting by using 5-10 times of 30-90% lower alcohol in volume for 2-3 times, each time for 2-3 h, mixing an extracting solution, concentrating in vacuum, and recycling a solvent to obtain an extract; dissolving the extract by adding water, sufficiently extracting by using ethyl acetate, mixing ethyl acetate extraction liquid, and concentrating in vacuum to obtain an ethyl acetate extract; carrying out silica gel column chromatography separation by using the ethyl acetate extract, carrying out gradient elute by using eluents, namely petroleum ether and ethyl acetate, concentrating eluents in vacuum with the volume ratio of petroleum ether to ethyl acetate of 60:40 or 40:60; and adding the concentrated solution into lower alcohol, concentrating and crystallizing in vacuum, filtering, and drying to obtain guaijaverin and avicularin. The method disclosed by the invention is simple and convenient; and guaijaverin and avicularin disclosed by the invention are capable of obviously reducing hyperglycemia induced by streptozotocin and can be used for preparing medicines or health-care products for reducing blood glucose.

Description

technical field [0001] The invention relates to the field of hypoglycemic health care products, in particular to a method and application for simultaneously preparing guava glycoside and guangparasitin from guava leaves. Background technique [0002] Diabetes (Diabetes Mellitus, DM) is caused by the decline of pancreatic islet function, insufficient insulin secretion and insulin resistance caused by a single or multiple pathogenic factors acting on the body, and is characterized by high blood sugar levels, accompanied by " Metabolic disorder syndrome with three excesses and one deficiency symptoms. With the increasing incidence of diabetes, it has become the third major chronic disease that seriously endangers human health after cardiovascular and cerebrovascular diseases and tumors in the world. [0003] Guava (Psidium guajava Linn.) is a Myrtaceae (Myrtaceae) guava plant, native to America, widely distributed in America, Asia and other tropical and subtropical regions, in...

AI Technical Summary

Problems solved by technology

"Drug preparation for hypoglycemia and its preparation method" (201110147738.7) disclosed in Chinese patent application provides a pharmaceutical preparation with hypoglycemic effect, but it does not describe the specific active ingredients of the guava extract and its antihyperglycemic effect. Extraction process

The laboratory’s previous research has confirmed the material basis of the hypoglycemic active ingredients in guava leaves through in vitro experiments, but the extraction process of the hypoglycemic active ingredients in guava leaves is less publicly disclosed, and there is no industrial process, and most of them are isolated and extracted in the laboratory

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