Method for preparing guaijaverin and avicularin from guava leaf and use of method
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A technology of guava glycosides and guava leaves, which is applied in the directions of chemical instruments and methods, food preparation, sugar derivative preparation, etc.
Inactive Publication Date: 2015-03-25
SOUTH CHINA AGRI UNIV
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"Drug preparation for hypoglycemia and its preparation method" (201110147738.7) disclosed in Chinese patent application provides a pharmaceutical preparation with hypoglycemic effect, but it does not describe the specific active ingredients of the guava extract and its antihyperglycemic effect. Extraction process
The laboratory’s previous research has confirmed the material basis of the hypoglycemic active ingredients in guava leaves through in vitro experiments, but the extraction process of the hypoglycemic active ingredients in guava leaves is less publicly disclosed, and there is no industrial process, and most of them are isolated and extracted in the laboratory
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Embodiment 1
[0023] Weigh 10kg of guava leaf raw material, extract 3 times with 80L 50% low-carbon alcohol, 2 hours each time, concentrate the extract in vacuum to obtain extract extract; disperse the extract extract in water, extract 3 times with ethyl acetate, combine Concentrate the extract to obtain 400 g of ethyl acetate extract, pass the extract through a 100-mesh silica gel column, and the volume ratio of petroleum ether and ethyl acetate is 100:0, 80:20, 60:40, 50:50, 40:60 , 20:80, 0:100, each ratio of 5BV, the flow rate of elution is 1 / 2BV / h, vacuum concentration 60:40, 40:60 eluate, 50% ethanol crystallization to obtain guaijaniside 20.6g, Guang Parasitin 21.3g, liquid phase detection content guaijagarin 92.5%, wide parasitin 95.7%.
Embodiment 2
[0025] Weigh 50kg of guava leaf raw material, extract 3 times with 300L 50% low-carbon alcohol, 2 hours each time, and concentrate the extract in vacuum to obtain extract extract; the extract extract is dispersed in water, extracted 3 times with ethyl acetate, combined Concentrate the extract to obtain 12kg of ethyl acetate extract, pass the extract through a 200-mesh silica gel column, and the volume ratio of petroleum ether and ethyl acetate is 100:0, 80:20, 60:40, 50:50, 40:60 , 20:80, 0:100, each ratio of 5BV, the flow rate of elution is 1 / 3BV / h, vacuum concentration 60:40, 40:60 eluate, 40% ethanol crystallization to obtain guaijaniside 101.6g, Guang 103.5g of parasitic glycosides, liquid phase detection content of guaijagarin 93.1%, wide parasitin 97.4%.
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Abstract
The invention provides a method for simultaneously preparing guaijaverin and avicularin from guava leaf and use of the method. The method provided by the invention comprises the following steps: crushing guava leaf as the raw material, extracting by using 5-10 times of 30-90% lower alcohol in volume for 2-3 times, each time for 2-3 h, mixing an extracting solution, concentrating in vacuum, and recycling a solvent to obtain an extract; dissolving the extract by adding water, sufficiently extracting by using ethyl acetate, mixing ethyl acetate extraction liquid, and concentrating in vacuum to obtain an ethyl acetate extract; carrying out silica gel column chromatography separation by using the ethyl acetate extract, carrying out gradient elute by using eluents, namely petroleum ether and ethyl acetate, concentrating eluents in vacuum with the volume ratio of petroleum ether to ethyl acetate of 60:40 or 40:60; and adding the concentrated solution into lower alcohol, concentrating and crystallizing in vacuum, filtering, and drying to obtain guaijaverin and avicularin. The method disclosed by the invention is simple and convenient; and guaijaverin and avicularin disclosed by the invention are capable of obviously reducing hyperglycemia induced by streptozotocin and can be used for preparing medicines or health-care products for reducing blood glucose.
Description
technical field [0001] The invention relates to the field of hypoglycemic health care products, in particular to a method and application for simultaneously preparing guava glycoside and guangparasitin from guava leaves. Background technique [0002] Diabetes (Diabetes Mellitus, DM) is caused by the decline of pancreatic islet function, insufficient insulin secretion and insulin resistance caused by a single or multiple pathogenic factors acting on the body, and is characterized by high blood sugar levels, accompanied by " Metabolic disorder syndrome with three excesses and one deficiency symptoms. With the increasing incidence of diabetes, it has become the third major chronic disease that seriously endangers human health after cardiovascular and cerebrovascular diseases and tumors in the world. [0003] Guava (Psidium guajava Linn.) is a Myrtaceae (Myrtaceae) guava plant, native to America, widely distributed in America, Asia and other tropical and subtropical regions, in...
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