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Molecular detection method of salmonella and application thereof

A Salmonella and molecular detection technology, applied in the biological field, can solve the problems of low sensitivity, high false positive results, easy to produce precipitation, etc., and achieve the effect of short time consumption and simple operation

Inactive Publication Date: 2015-03-25
INSPECTION & QUARANTINE TESTING CENT OF HEBEI ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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Problems solved by technology

At present, the relatively mature single-primer isothermal amplification technology uses viruses as the object, and the detection method after amplification is polyacrylamide gel electrophoresis. The detection sensitivity of this method is low, precipitation is easy to occur, and the result is false positive. High, especially not suitable for amplification detection of prokaryotic microorganisms

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  • Molecular detection method of salmonella and application thereof
  • Molecular detection method of salmonella and application thereof
  • Molecular detection method of salmonella and application thereof

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Embodiment Construction

[0032] In order to make the above objects, features and advantages of the present invention more obvious and easy to understand, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings and specific implementation methods in the embodiments of the present invention. Obviously, The described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0033] The following experiments were completed by the biological laboratory of the Inspection and Quarantine Technology Center of Hebei Entry-Exit Inspection and Quarantine Bureau.

[0034] 1 Materials and methods

[0035] 1.1 Materials

[0036] 1.1.1 Strains

[0037] Salmonella Typhimurium, Salmonella Senfutenburg,...

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Abstract

The invention relates to a molecular detection method of salmonella. According to the molecular detection method, a single primer isothermal amplification method is adopted, a specific sequence of a salmonella invA gene is taken as a target sequence, SYBER Green II is taken as a fluorescent dye, and amplification signals are detected through real-time fluorescent quantitative PCR (Polymerase Chain Reaction). The invention further relates to application of the molecular detection method. The detection method disclosed by the invention is high in sensitivity, strong in specificity and sensibility and short in consumed time, has relatively low requirements on template DNA, and is simple and convenient.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a molecular detection method for Salmonella and an application thereof. Background technique [0002] Salmonella is one of the most common pathogens causing microbial food poisoning, especially in fresh meat, eggs, milk and other livestock and poultry products ， . In Europe, more than 160,000 people are infected with Salmonella every year, with an incidence of about 0.035%. In the United States, more than 1.4 million people are infected with Salmonella each year, and the direct economic loss exceeds 2.4 billion US dollars. In China, food poisoning incidents caused by Salmonella account for 40%~60% of all food poisoning incidents every year. Hygiene is very important. Generally speaking, ingestion of highly pathogenic serotype Salmonella 2.0×10 5 cfu / g can cause disease, weak pathogenicity of serotype Salmonella 10 8 CFU / g to cause food poisoning. Typically, 2.0×10 ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12Q1/10C12R1/42
CPCC12Q1/6844C12Q2531/101C12Q2561/113C12Q2561/101C12Q2525/117
Inventor 李静王建昌孙晓霞李瑞王金凤
Owner INSPECTION & QUARANTINE TESTING CENT OF HEBEI ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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