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Tissue culture and rapid propagation method for isatis tinctoria

A technique for tissue culture rapid propagation and woad indigo, applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problems of low seedling induction rate, failure to meet routine experiments, production needs, etc., to ensure the induction effect, shorten the Effects of emergence time and shortening induction time

Inactive Publication Date: 2015-04-08
TIANJIN CITY AGRI BIO TECH RES CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The current disadvantage is that the emergence induction rate is low, which cannot meet the needs of routine experiments and production.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] (1) Obtaining Isatis indigo sterile vaccine:

[0030] Take Isatis indica seeds, peel off the seed coat, put them into a sterilized 50mL conical flask on a clean bench, add 10mL of 75% ethanol to soak for 30S, pour out the ethanol, add 2.5% sodium hypochlorite solution, disinfect for 10min, pour out Sodium hypochlorite and sterile water were washed 5 times, and the seeds were taken out and placed in a 100mL sterilized Erlenmeyer flask containing 20mL of MS medium. The flask was placed in a light incubator at 25°C with a light intensity of 1200 lux and 16 hours of light every day. After 3 days, the seeds germinated, and after about 30 days, the woad seedlings grew to 10 cm.

[0031] (2) Acquisition of clustered buds:

[0032] On the ultra-clean workbench, cut the leaves of Isatis indigo into 1cm-long sections and put them in a 100mL Erlenmeyer flask filled with 20mL cluster bud medium (MS + 1.3 mg / L 6-BA + 0.5 mg / L NAA). The flask was placed in a light incubator at 22±...

Embodiment 2

[0038] (1) Obtaining sterile Isatis indigo vaccine:

[0039] Take Isatis indica seeds, peel off the seed coat, put them into a sterilized 50mL Erlenmeyer flask on an ultra-clean workbench, add 10mL of 75% ethanol to soak for 30S, pour out the ethanol, add 2.5% sodium hypochlorite solution, sterilize for 11min, pour out Sodium hypochlorite and sterile water were washed 5 times, and the seeds were taken out and placed in a 100mL sterilized Erlenmeyer flask containing 20mL of MS medium. The flask was placed in a light incubator at 25°C with a light intensity of 1200 lux and 16 hours of light every day. After 4 days, the seeds germinated, and after about 30 days, the woad seedlings grew to 10 cm.

[0040] (2) Acquisition of clustered buds:

[0041] Cut Isatis indigo leaves into 1.5cm-long sections on the ultra-clean workbench and put them in a sterilized 100mL Erlenmeyer flask containing 20mL cluster bud medium (MS+ 1.4mg / L 6-BA+ 0.6 mg / L NAA), Place the flask in a light incuba...

Embodiment 3

[0047] (1) Obtaining sterile Isatis indigo vaccine:

[0048] Take Isatis indica seeds, peel off the seed coat, put them into a sterilized 50mL Erlenmeyer flask on an ultra-clean workbench, add 10mL of 75% ethanol to soak for 30S, pour out the ethanol, add 2.5% sodium hypochlorite solution, sterilize for 12min, pour out Sodium hypochlorite and sterile water were washed 5 times, and the seeds were taken out and placed in a 100mL sterilized Erlenmeyer flask containing 20mL of MS medium. The flask was placed in a light incubator at 25°C with a light intensity of 1200 lux and 16 hours of light every day. After 3 days, the seeds germinated, and after about 30 days, the woad seedlings grew to 10cm.

[0049] (2) Acquisition of clustered buds:

[0050] Cut Isatis indigo leaves into 2 cm long pieces on the ultra-clean workbench and put them in a sterilized 100mL Erlenmeyer flask containing 20mL cluster bud medium (MS+ 1.5 mg / L 6-BA + 0.7 mg / L NAA), Place the flask in a light incubato...

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Abstract

The invention discloses a tissue culture and rapid propagation method for isatis tinctoria. The tissue culture and rapid propagation method comprises the following steps: obtaining isatis-tinctoria aseptic seedlings, obtaining clustered shoots, carrying out rooting culture and hardening seedlings. The tissue culture and rapid propagation method disclosed by the invention has the advantages that since isatis-tinctoria seeds with the skins being peeled are adopted as experimental materials, the seedling emergence time is shortened and the seedling emergence rate is increased; MS+1.3-1.7mg / L 6-BA and 0.5-1.0mg / L NAA are adopted as clustered-shoot induction media, so that the clustered-shoot induction time is shortened, the clustered-shoot induction rate is increased and the guarantee is provided for obtaining a great quantity of clustered shoots in short time; 1 / 2MS+0.3-0.7mg / L IBA is adopted as a rooting medium, so that a great quantity of thin-long and strong root systems are obtained, and a foundation is laid for surviving of the young seedlings; and since a mixture of vermiculite, turf and perlite is adopted as a medium in the hardening, the survival rate of the young seedlings is guaranteed.

Description

technical field [0001] The invention relates to the field of tissue culture and rapid propagation of medicinal plants, in particular to a method for tissue culture and rapid propagation of Isatis indica, a plant of the genus Isatis in the family Cruciferae. Background technique [0002] Woad ( Isatis indigotica Fortune), a biennial herb of the Cruciferae family, is native to my country and has a long history of cultivation. It is cultivated in most parts of the north, but relatively few in the south. The roots and leaves of Isatis indigo are used for medicinal purposes, and have the effects of clearing away heat and detoxification, cooling blood and eliminating spots, relieving sore throat and relieving pain. Blue dye can also be extracted from the leaves; oil is extracted from the seeds for industrial use. The root of woad is called Banlangen ( Radix isatidis ), which is a traditional Chinese herbal medicine in my country, recorded in "Shen Nong's Materia Medica", and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 刘玉堂岳东霞赵宪争尉万聪杨迎霞周祥明宋兆伟
Owner TIANJIN CITY AGRI BIO TECH RES CENT
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