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Endotoxin adsorbent for hemoperfusion and preparation method thereof

A blood perfusion and endotoxin technology, applied in chemical instruments and methods, other chemical processes, dialysis systems, etc., can solve the problem of low ability to absorb endotoxin, avoid harmful substances residue, increase ligand content, epoxy resin The effect of increasing the base content

Active Publication Date: 2016-10-05
佛山市博新生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, this adsorbent material is less capable of absorbing endotoxin

Method used

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  • Endotoxin adsorbent for hemoperfusion and preparation method thereof

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Comparison scheme
Effect test

Embodiment 1

[0046] The preparation process of the polymethacrylate adsorbent of the present embodiment is as follows:

[0047] 1-1 Polymethacrylate adsorbent

[0048] 1-1-1 prepare porous carrier;

[0049] Prepare polymethyl methacrylate resin, which is prepared by suspension polymerization, with a pore size of 5-50 nm and a specific surface area of ​​600 m 2 / g, the residual double bond content is 1.2mmol / g.

[0050] 1-1-2 Epoxy modification

[0051] Take 10g of polymethyl methacrylate resin, add 40mL of dichloroethane to soak overnight, then add 20mL of 1% m-chloroperoxybenzoic acid in dichloroethane dropwise, place in an ice-water bath (the temperature is controlled at 0~ 4°C) Stir the reaction for 24h at a rotation speed of 200rpm. After the reaction was completed, it was washed with alcohol and purified water; an epoxy-modified polymethyl methacrylate resin was obtained, and the epoxy group content of the resin was tested to be 0.7 mmol / g.

[0052] 1-1-3 Immobilized Ligands (Pol...

Embodiment 2

[0070] The preparation process of the polystyrene divinylbenzene resin adsorbent of the present embodiment is as follows:

[0071] 2-1 Prepare a porous carrier;

[0072] Prepare polystyrene divinylbenzene resin, using divinylbenzene with a purity of 80wt%, and prepare it by suspension polymerization. The obtained resin has a pore size of 5-50nm and a specific surface area of ​​800m 2 / g, the residual double bond content is 2.1mmol / g.

[0073] 2-2 Epoxy modification

[0074] Take 10g of polystyrene divinylbenzene resin and add 40mL of dichloroethane to soak overnight, then add 20mL of 1% m-chloroperoxybenzoic acid in dichloroethane dropwise, and put it in an ice-water bath (the temperature is controlled at 0~4 °C) Stir the reaction for 24 hours at a rotation speed of 200 rpm. After the reaction is completed, wash with alcohol and purified water. An epoxidation-modified polystyrene divinylbenzene resin was obtained, and the epoxy group content of the resin was tested to be 1...

Embodiment 3

[0083] The preparation process of the polystyrene divinylbenzene resin adsorbent of the present embodiment is as follows:

[0084] 3-1 Preparation of Porous Support

[0085] Prepare polystyrene divinylbenzene resin, using divinylbenzene with a purity of 80wt%, and prepare it by suspension polymerization. The obtained resin has a pore size of 5-50nm and a specific surface area of ​​800m 2 / g, the residual double bond content is 2.2mmol / g.

[0086] 3-2 Graft modification

[0087] 3-2-1 grafted vinyl acetate

[0088] Take 10g of polystyrene divinylbenzene resin, add 50mL of 95% alcohol solution of 1.5% vinyl acetate, and 0.12g of dibenzoyl peroxide. Start stirring speed 200rpm. The reaction was carried out at 70° C. for 4 hours. After the reaction is completed, wash with alcohol and purified water.

[0089] 3-2-2 grafted vinylpyrrolidone and hydroxyethyl methacrylate

[0090] Take 10g of polystyrene divinylbenzene resin, add 1% vinylpyrrolidone, 50mL of 95% alcohol solutio...

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Abstract

The invention discloses an endotoxin adsorbent used in hemoperfusion, and a preparation method thereof. The preparation method comprises the following steps: a porous carrier is prepared; the carrier is polystyrene divinylbenzene resin or polymethyl methacrylate resin, wherein the pore size of the resin is 5-50nm and the specific surface area of the resin is no less than 500m<2> / g; residual double bonds of the carrier resin are subjected to epoxidation modification; and ligands are immobilized. According to the scheme of the invention, a synthesis process route is changed, such that adsorbent harmful substance residue is avoided, and adsorbent adsorption capacity is improved. Specifically, residual double bonds of the adsorption resin are subjected to epoxidation modification, such that the epoxy group amount is increased; with the epoxy groups, hydrophobic tethers are added, such that ligand movement capacity is improved, and the adsorbent is provided with a good endotoxin adsorption effect; and resin pore size and specific surface area are controlled, such that the adsorption amount and removal rate of the adsorbent upon cytokines are ensured.

Description

technical field [0001] The invention relates to an endotoxin adsorbent for hemoperfusion and a preparation method thereof. Background technique [0002] Endotoxemia can occur in multiple diseases of multiple systems, usually leading to sepsis, fatal septic shock, multiple organ failure, disseminated intravascular coagulation, etc., and the fatality rate is extremely high, exceeding 40%. The endotoxin adsorbent for hemoperfusion can be used to remove endotoxins in patients with sepsis, septic shock and multiple organ dysfunction syndrome, so as to improve the survival rate of patients and improve their clinical conditions. [0003] The endotoxin adsorbent for hemoperfusion is usually obtained by using macroporous resin or activated carbon as the carrier, and loading the ligand through activation. At present, the commonly used ligands for adsorbing endotoxin mainly include polymyxin B, polylysine, human serum albumin, polyethyleneimine PEI, diethylaminoethyl DEAE, anti-rHu-TN...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01J20/26B01J20/30A61M1/14
CPCB01J20/26
Inventor 姜建明李设桥
Owner 佛山市博新生物科技有限公司
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