Method for detecting 6-benzyladenine and 4-sodium chlophenoxycetate in bean sprouts at the same time
A technology of sodium chlorophenoxyacetate and benzyl adenine, which is applied to measurement devices, instruments, scientific instruments, etc., can solve the problems of poor sensitivity, multiple resources, and difficulty in meeting high-sensitivity detection requirements, and achieves the effect of high sensitivity
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Embodiment 1
[0037] (1) Pretreatment process
[0038] Take the bean sprouts sample to be tested and put it into a tissue crusher for crushing. Take 10g of the crushed sample and put it in a 50mL centrifuge tube with a screw cap; add 5mL of 10mM sodium hydroxide aqueous solution and 5mL of methanol solution, shake well, and carry out Light wave extraction for 2 minutes (pay attention to sufficient light), then centrifuge at 8000rpm for 10min; take 1mL of the supernatant, put it in a 1.5mL centrifuge tube, centrifuge at 12000rpm for 10min, take the supernatant and filter it with a 0.45μm filter membrane, and put it into an automatic sampling bottle to be tested.
[0039] (2) Put the liquid to be tested into the liquid chromatography tandem mass spectrometer for detection
[0040] HPLC conditions
[0041] Chromatographic column: Eclipse Plus C18 (2.1 mm×150 mm, 3.1 μm, Agilent Company). Mobile phase: A phase is 0.1% formic acid water, and B phase is acetonitrile. Gradient elution program:...
Embodiment 2
[0047] The test procedure is the same as in Example 1, the difference is that the light wave extraction time is continuously increased from 10s to 2.5min, and the target peak area changes as follows: figure 2 .
Embodiment 3
[0049] The test procedure is the same as in Example 1, the difference is that the fragmentation voltage is changed from 10V to 150V, the mass-to-charge ratio of the parent ion and different collision energies (0V to 40V),
[0050] The experimental method is as follows: First, in the mode of selected ion detection, input the mass-to-charge ratio of the sample precursor ion, optimize the fragmentation voltage (10V ~ 120V), ensure the maximum transmission efficiency of the precursor ion, that is, reach the collision cell as much as possible, the specific experiment see results figure 1 , figure 2 Then, in the product ion scanning mode, input the mass-to-charge ratio of the parent ion and different collision energies (0V ~ 40V), investigate the influence of different collision energies on the peak intensity of the parent ion and the product ion, the selected parent ion almost disappears, and the product ion intensity Collision energy at maximum.
[0051] Linear relationship and...
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