Method for detecting 6-benzyladenine and 4-sodium chlophenoxycetate in bean sprouts at the same time

A technology of sodium chlorophenoxyacetate and benzyl adenine, which is applied to measurement devices, instruments, scientific instruments, etc., can solve the problems of poor sensitivity, multiple resources, and difficulty in meeting high-sensitivity detection requirements, and achieves the effect of high sensitivity

Active Publication Date: 2015-04-22
SHANDONG ANALYSIS & TEST CENT +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The current detection methods for 6-BA and 4-CPA in bean sprouts have the disadvantages of cumbersome pretreatment methods, poor sensitivity, and can only detect a single component. For example, the standard "GB / T23381-2009" issued by the National Standards Committee is only for 6-BA for detection, and the detection limit of the method is high, it is difficult to meet the high sensitivity detection requirements
Relevant departments of the country are also actively developing detection methods for multi-component plant growth regulators, for example, the local standard "DB33 / T 625.3-2007" in Zhejiang Province, however, this standard uses different methods for 6-BA and 4-CPA Pre-processing techniques and instrumental analysis methods will inevitably increase detection tasks and consume more resources

Method used

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  • Method for detecting 6-benzyladenine and 4-sodium chlophenoxycetate in bean sprouts at the same time
  • Method for detecting 6-benzyladenine and 4-sodium chlophenoxycetate in bean sprouts at the same time
  • Method for detecting 6-benzyladenine and 4-sodium chlophenoxycetate in bean sprouts at the same time

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] (1) Pretreatment process

[0038] Take the bean sprouts sample to be tested and put it into a tissue crusher for crushing. Take 10g of the crushed sample and put it in a 50mL centrifuge tube with a screw cap; add 5mL of 10mM sodium hydroxide aqueous solution and 5mL of methanol solution, shake well, and carry out Light wave extraction for 2 minutes (pay attention to sufficient light), then centrifuge at 8000rpm for 10min; take 1mL of the supernatant, put it in a 1.5mL centrifuge tube, centrifuge at 12000rpm for 10min, take the supernatant and filter it with a 0.45μm filter membrane, and put it into an automatic sampling bottle to be tested.

[0039] (2) Put the liquid to be tested into the liquid chromatography tandem mass spectrometer for detection

[0040] HPLC conditions

[0041] Chromatographic column: Eclipse Plus C18 (2.1 mm×150 mm, 3.1 μm, Agilent Company). Mobile phase: A phase is 0.1% formic acid water, and B phase is acetonitrile. Gradient elution program:...

Embodiment 2

[0047] The test procedure is the same as in Example 1, the difference is that the light wave extraction time is continuously increased from 10s to 2.5min, and the target peak area changes as follows: figure 2 .

Embodiment 3

[0049] The test procedure is the same as in Example 1, the difference is that the fragmentation voltage is changed from 10V to 150V, the mass-to-charge ratio of the parent ion and different collision energies (0V to 40V),

[0050] The experimental method is as follows: First, in the mode of selected ion detection, input the mass-to-charge ratio of the sample precursor ion, optimize the fragmentation voltage (10V ~ 120V), ensure the maximum transmission efficiency of the precursor ion, that is, reach the collision cell as much as possible, the specific experiment see results figure 1 , figure 2 Then, in the product ion scanning mode, input the mass-to-charge ratio of the parent ion and different collision energies (0V ~ 40V), investigate the influence of different collision energies on the peak intensity of the parent ion and the product ion, the selected parent ion almost disappears, and the product ion intensity Collision energy at maximum.

[0051] Linear relationship and...

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Abstract

The invention relates to a method for detecting 6-benzyladenine and 4-sodium chlophenoxycetate in bean sprouts at the same time. Breaking treatment is carried out on the bean sprouts to be detected, the broken bean sprouts to be detected are placed in a vessel, a sodium hydroxide aqueous solution and a methanol solution are added into the vessel, after the solutions are shaken up, light wave extraction is carried out for 10 seconds to 3 minutes, high-speed centrifugal treatment is carried out, a liquid supernatant is taken and filtered through a film to obtain liquid to be detected, and the liquid to be detected enters a liquid chromatography tandem mass spectrometer to be detected. The method has the advantages of being rapid, efficient, high in sensitivity and the like, and provides the new guarantee for safety monitoring of quality of the bean sprouts in the market.

Description

technical field [0001] The invention relates to a detection method of a growth regulator in bean sprouts. Background technique [0002] my country's bean sprouts are mainly produced in workshops. In order to pursue high profits, plant growth regulators are added indiscriminately in the bean sprouts production process to stimulate the growth of bean sprouts. Among them, 4-chlorophenoxyacetic acid (4-CPA) and 6-benzyl adenine (6-BA) are the main components of "AB powder" and "rootless bean sprouts" that are more commonly used in the cultivation of problem bean sprouts. 4-CPA is a systemic plant growth regulator, which can regulate the balance of plant hormones, stimulate ovary expansion, supplement the lack of auxin in plants, and promote growth. 6-BA is similar to plant endogenous hormones in structure and properties, and can promote the formation of sprouts and inhibit the growth of roots during the growth of bean sprouts. Excessive intake of 6-BA by the human body will st...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88
Inventor 赵汝松邢寒竹苑金鹏陈跃王珊珊王晓利
Owner SHANDONG ANALYSIS & TEST CENT
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