Construction method and sequencing method of genetic typing sequencing library
A technology for genotyping, sequencing, and construction methods, which is applied in chemical libraries, biochemical equipment and methods, and microbial determination/inspection, etc. problem, to achieve the effect of improving the effective amount, improving the efficiency of building the library, and increasing the length
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[0021] It should be noted that, in the case of no conflict, the embodiments in the present application and the features in the embodiments can be combined with each other. The present invention will be described in detail below with reference to the accompanying drawings and examples.
[0022] In view of the cumbersome process of building a library in the construction method of the genotyping sequencing library in the prior art, the time is long, and the quality of the sequencing data obtained by sequencing the constructed library is not high, resulting in data waste and other shortcomings, in a typical method of the present invention In an embodiment, a method for constructing a genotyping sequencing library is provided, such as figure 1 As shown, the method includes the following steps: S1, when the genomic DNA is digested for the first time to generate the first restriction fragment, at the same time, a sample tag sequence is connected to both ends of the first restriction ...
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