A method for the determination of three isothiazolinone preservatives in water-based glue by liquid chromatography-tandem mass spectrometry
A technology of isothiazolinone and tandem mass spectrometry, which is applied in the field of physical and chemical inspection of water-based adhesives, and achieves the effect of fast operation, simple operation and high sensitivity
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[0018] The present invention is further described by the following specific examples, but does not limit the present invention.
[0019] 1. Instruments and reagents
[0020] Instruments: ThermoScientificTSQQuantumUltra triple quadrupole mass spectrometer, ThermoScientificSurveyorPlus high performance liquid chromatography system (ThermoFisher company in the United States); CORTECSC18+ chromatographic column (2.1mm×100mm, 2.7μm, American Waters company); Sigma4-16K high-speed centrifuge (German Sigma company); KQ500-DB ultrasonic generator (Jiangsu Kunshan Ultrasonic Instrument Co., Ltd.); Milli-Q ultrapure water system (U.S. Millipore company); XP205 electronic balance (sensing Quantity: 0.0001g, Mettler-Toledo, Switzerland).
[0021] Reagents: 2-methyl-4-isothiazolin-3-one (MI), 5-chloro-2-methyl-isothiazolin-3-one (CMI), 1,2-benzisothiazolyl- 3(2H)-ketone (BIT) (purity ≥98.0%, Dr.Ehrenstorfer, Germany), internal standard 1,2-benzisothiazolyl-3(2H)-one- 13 C 6 (BIT- 13 C 6 ) (Puri...
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