Gene mapping multi-inspection method

A technology of gene positioning and testing methods, applied in the field of bioinformatics, to achieve the effect of improving precision and accuracy, improving utilization rate, and shortening cycle

Active Publication Date: 2015-04-29
HANGZHOU HEYI GENE TECH
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

Previously traditional markers (such as SSR, RFLP, etc.) could not realize the possibility of performing two analyzes simultaneously in one independent experiment

Method used

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  • Gene mapping multi-inspection method

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Embodiment Construction

[0027] Embodiments of the present invention are described in further detail below in conjunction with accompanying drawings:

[0028] The invention discloses a multiple testing method for gene positioning, which is characterized in that it comprises:

[0029] Step 1: Sequence the parental DNA samples and offspring DNA samples using second-generation sequencing technology to obtain high-precision short-segment sequences;

[0030] Step 2: using SNP analysis software to compare or cluster the obtained high-precision short fragment sequences with reference sequences to obtain accurate SNP information for each sample;

[0031] Step 3: convert such SNP information into population SNP, further convert into standard molecular standard format, use mapping software to construct genetic map, and perform QTL analysis on the basis of genetic map to obtain QTL locus information;

[0032] Step 4: Identify the phenotypic information of the progeny DNA samples, and accurately count the distri...

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Abstract

The invention discloses a gene mapping multi-inspection method. The method is characterized by comprising the steps of 1, sequencing a parent DNA sample and a filial generation DNA sample to obtain a high-precision short segment sequence; 2,comparing the obtained high-precision short segment sequence with a reference sequence, or mutually clustering to obtain accurate SNP information; 3, converting the SNP information into group SNP, further converting into a standard molecular standard format, creating a genetic map, and performing QTL analysis on the basis of the genetic map to obtain QTL seat information; 4, identifying the phenotype information of filial generation DNA sample, accurately counting the phenotype value distribution, grouping the phenotype values according to gradient, respectively blending the high-precision short segment sequences of the filial generation DNA sample, corresponding to the extreme phenotype values, analyzing the genotype frequency distribution, and performing chi-square test and rank sum test to find out related obvious areas; 5, integrating the QTL seat information and the accurate gene mapping information obtained in step 4.

Description

technical field [0001] The invention relates to the technical field of biological information, in particular to a multiple testing method for gene positioning. Background technique [0002] The simplified genome method is a technical method that uses enzyme digestion technology, sequence capture chip technology or other experimental methods to reduce the complexity of species genomes, and then study various genetic structural variations of genomes. Common simplified genome technologies include RAD (Restriction site Associated DNA) and GBS (Genotyping By Sequencing). These technologies can develop tens of thousands of SNP markers in a very short period of time, and molecular markers are the basis for genetic mapping, association analysis, population genetic analysis, and ecological diversity analysis. Scientific research is a popular application of current second-generation sequencing technology. [0003] BSA (Bulked Segregation Analysis or Bulked Segregation Analysis) was ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G06F19/22C12Q1/68
Inventor 刘三阳范崇仪钱晓菊张新明高金龙罗亚丹范玉美赵艳艳王兆宝
Owner HANGZHOU HEYI GENE TECH
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