Gene mapping multi-inspection method

A technology of gene positioning and testing methods, applied in the field of bioinformatics, to achieve the effect of improving precision and accuracy, improving utilization rate, and shortening cycle

CN104573409AActive Publication Date: 2015-04-29HANGZHOU HEYI GENE TECH

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Patent Information

Authority / Receiving Office: CN · China
Patent Type: Applications(China)
Current Assignee / Owner: HANGZHOU HEYI GENE TECH
Publication Date: 2015-04-29

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Abstract

The invention discloses a gene mapping multi-inspection method. The method is characterized by comprising the steps of 1, sequencing a parent DNA sample and a filial generation DNA sample to obtain a high-precision short segment sequence; 2,comparing the obtained high-precision short segment sequence with a reference sequence, or mutually clustering to obtain accurate SNP information; 3, converting the SNP information into group SNP, further converting into a standard molecular standard format, creating a genetic map, and performing QTL analysis on the basis of the genetic map to obtain QTL seat information; 4, identifying the phenotype information of filial generation DNA sample, accurately counting the phenotype value distribution, grouping the phenotype values according to gradient, respectively blending the high-precision short segment sequences of the filial generation DNA sample, corresponding to the extreme phenotype values, analyzing the genotype frequency distribution, and performing chi-square test and rank sum test to find out related obvious areas; 5, integrating the QTL seat information and the accurate gene mapping information obtained in step 4.

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Description

technical field

[0001] The invention relates to the technical field of biological information, in particular to a multiple testing method for gene positioning. Background technique

[0002] The simplified genome method is a technical method that uses enzyme digestion technology, sequence capture chip technology or other experimental methods to reduce the complexity of species genomes, and then study various genetic structural variations of genomes. Common simplified genome technologies include RAD (Restriction site Associated DNA) and GBS (Genotyping By Sequencing). These technologies can develop tens of thousands of SNP markers in a very short period of time, and molecular markers are the basis for genetic mapping, association analysis, population genetic analysis, and ecological diversity analysis. Scientific research is a popular application of current second-generation sequencing technology.

[0003] BSA (Bulked Segregation Analysis or Bulked Segregation Analysis) was ...

Claims

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