Multiple testing methods for gene mapping

A technology of gene positioning and testing methods, applied in the field of bioinformatics, to achieve the effect of improving precision and accuracy, improving utilization rate, and shortening cycle
CN104573409BActive Publication Date: 2017-07-25HANGZHOU HEYI GENE TECH
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
HANGZHOU HEYI GENE TECH
Publication Date
2017-07-25

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Abstract

The invention discloses a multiple detection method for gene positioning, which is characterized in that it comprises: step 1: sequence the DNA samples of parents and offspring to obtain high-precision short fragment sequences; step 2: sequence the obtained high-precision DNA samples The short fragment sequence is compared with the reference sequence or clustered with each other to obtain accurate SNP information; Step 3: convert the SNP information into a population SNP, and further convert it into a standard molecular standard format to construct a genetic map, and on the basis of the genetic map Step 4: Identify the phenotypic information of the progeny DNA samples, and accurately count the distribution of phenotypic values, group the phenotypic values ​​according to gradients, and group the extreme phenotypic values ​​corresponding to the offspring The high-precision short fragment sequences of the first-generation DNA samples are mixed separately, the genotype frequency distribution is analyzed, and the relevant significant regions are found through the chi-square test and the rank sum test; Step 5: Integrate the QTL locus information and Step 4 to obtain accurate gene positioning information.
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Description

technical field

[0001] The invention relates to the technical field of biological information, in particular to a multiple testing method for gene positioning. Background technique

[0002] The simplified genome method is a technical method that uses enzyme digestion technology, sequence capture chip technology or other experimental methods to reduce the complexity of species genomes, and then study various genetic structural variations of genomes. Currently common simplified genome technologies include RAD (Restriction site Associated DNA) and GBS (Genotyping By Sequencing). These technologies can develop tens of thousands of SNP markers in a very short period of time, and molecular markers are the basis for genetic mapping, association analysis, population genetic analysis, and ecological diversity analysis. Scientific research is a popular application of current second-generation sequencing technology.

[0003] BSA (Bulked Segregation Analysis or Mixed Segregation Analy...

Claims

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