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Method for separating and purifying derivative of pinobanksin and caffeic acid from Chinese propolis aqueous extract

A technique for separating and purifying raffinin, which is applied in the field of chemical industry, can solve the problems of few reports on the research on chemical components of water-soluble parts, and achieves the effects of saving reagents, simple operation and high purity

Inactive Publication Date: 2015-05-06
LIAOCHENG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Because propolis is easily soluble in ethanol, there are many reports on the chemical components of the fat-soluble parts of Chinese propolis, but there are relatively few reports on the chemical components of the water-soluble parts.

Method used

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  • Method for separating and purifying derivative of pinobanksin and caffeic acid from Chinese propolis aqueous extract
  • Method for separating and purifying derivative of pinobanksin and caffeic acid from Chinese propolis aqueous extract
  • Method for separating and purifying derivative of pinobanksin and caffeic acid from Chinese propolis aqueous extract

Examples

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Embodiment

[0037] Example: A method for isolating and purifying derivatives of brachypine and caffeic acid from the water-soluble part of Chinese propolis, the steps of which are as follows:

[0038] (1) Preparation of Chinese propolis water extract: freeze Chinese propolis, crush it, heat and extract it with 8 times the amount of distilled water for 4 times, each time for 2 hours, filter, cool the water extract naturally, remove the solidified beeswax in the upper layer, and then depressurize Concentrate to a relative density of about 1.2.

[0039] (2) Alcohol precipitation: add about 2 times the amount of 95% ethanol to the above concentrated solution until the ethanol content is about 70%, let it stand overnight, and take the supernatant and concentrate to obtain the extract.

[0040] (3) Coarse separation of macroporous adsorption resin: Add appropriate amount of water to dissolve the above extract, put it on HPD826 macroporous adsorption resin column, first elute with water, and t...

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Abstract

The invention relates to a method for separating and purifying derivative of pinobanksin and caffeic acid from Chinese propolis aqueous extract. Chinese propolis is used as the raw material and the method comprises the following steps: (1) preparing the Chinese propolis aqueous extract; (2) carrying out alcohol precipitation; (3) carrying out crude separation on the macroreticular resin; and (4) separating and purifying via semi preparation type high performance liquid chromatography. Semi preparation type high performance liquid chromatography is used for separating and purifying, the mobile phase is methanol-water, and four components with high purity can be prepared and are identified to be 3,4-dimethoxycinnamic acid, pinobanksin, caffeic acid benzyl ester and caffeic acid phenethyl ester respectively. The technology process is environment-friendly, no serous prejudice is generated to the environment and the comprehensive cost is low.

Description

technical field [0001] The invention belongs to the field of chemical industry, and in particular relates to a method for separating and purifying derivatives of brachypine and caffeic acid from Chinese propolis water extract. Background technique [0002] Chinese propolis has a wide range of pharmacological activities such as anti-tumor, anti-bacterial, anti-virus, anti-inflammatory, anti-oxidation, enhancing immunity, promoting tissue regeneration, protecting the liver, lowering blood fat, and lowering blood sugar. It has the reputation of "purple gold" and is widely used in food , health care products and cosmetics industry. Chinese propolis contains a variety of chemical components (such as phenolic acids, flavonoids, amino acids, steroids, etc.), of which phenolic acids and flavonoids are the main active components. Caffeic acid and its derivatives (such as benzyl caffeate, phenethyl caffeate, etc.) contained in Chinese propolis have anti-tumor, anti-oxidation, antibac...

Claims

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Application Information

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IPC IPC(8): C07D311/32C07D311/40C07C59/64C07C51/42C07C69/732C07C67/48
CPCC07C51/47C07C67/56C07D311/32C07D311/40C07C59/64C07C69/732
Inventor 李爱峰玄红专柳仁民孙爱玲
Owner LIAOCHENG UNIV
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