Ailanthus bark anti-algae agent and its preparation method and application

The technology of Ailanthus bark and algae inhibitor is applied in the field of Ailanthus bark algae inhibitor and preparation, and can solve the problems of high technical requirements for separation and extraction of allelopathic substances, influence on the allelopathic potential of aquatic plants, and limitation of the growth of aquatic plants, etc. Excellent algae inhibition performance, simple preparation method and high inhibition efficiency

Inactive Publication Date: 2016-08-24
TAISHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the growth of aquatic plants is severely restricted by seasons and climates. At the same time, the release of toxic substances such as algae toxins will affect the allelopathic potential of aquatic plants.
In addition, the separation and extraction of allelochemicals from aquatic plants has problems such as high technical requirements and high cost, so the use of aquatic plants to inhibit algae has certain limitations in practical applications

Method used

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  • Ailanthus bark anti-algae agent and its preparation method and application
  • Ailanthus bark anti-algae agent and its preparation method and application
  • Ailanthus bark anti-algae agent and its preparation method and application

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Experimental program
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Effect test

Embodiment 1

[0029] The preparation method of embodiment 1 algastatic agent of the present invention

[0030] (1) Wash the Ailanthus bark and dry it in an oven at 70-80°C for later use.

[0031] (2) The dried Ailanthus bark is pulverized with a pulverizer and passed through a 100-mesh sieve.

[0032] (3) Weigh 15-20 g of Ailanthi bark dry powder, and extract it with an organic solvent according to the Soxhlet extraction method, the extraction temperature is 60-80° C., and the extraction time is 5-6 hours. Described organic solvent is dehydrated alcohol.

[0033] (4) The extract obtained by Soxhlet extraction is placed in a rotary evaporator, and the ethanol is evaporated to dryness by decompression and concentration, and dried to a constant weight to obtain an anhydrous ethanol crude extract of Ailanthus bark.

Embodiment 2

[0034] Embodiment 2 The application of algastatic agent of the present invention

[0035] Experimental Materials:

[0036] Microcystis aeruginosa (M.aeruginosa FACHB-905, provided by the Freshwater Algae Species Bank of the Chinese Academy of Sciences).

[0037] The formula of BG11 medium is:

[0038] NaNO 3 1.5g / L, K 2 HPO 4 40mg / L, MgSO 4 ·7H 2 O 75mg / L, CaCl 2 2H 2 O 36mg / L, Citricacid 6mg / L, Ferric ammonium citrate 6mg / L, EDTANa 2 1mg / L, Na 2 CO 3 20mg / L, A 5 Solution 1mL / L. where A 5 The solution formula is: MnCl 2 4H 2 O 1.86g / L, ZnSO 4 ·7H 2 O 0.22g / L, Na 2 MoO 4 2H 2 O0.39g / L, H 3 BO 3 2.86g / L, CuSO 4 ·5H 2 O 0.08g / L, Co(NO 3 ) 2 ·6H 2 O 0.05g / L.

[0039] Microcystis aeruginosa was expanded and cultured before the experiment. The culture conditions are: photoperiod 14h / 10h, temperature (25±1)°C, shaking 3-5 times a day, so that Microcystis aeruginosa enters logarithmic growth phase.

[0040] Use the absolute ethanol crude extract of Ailant...

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Abstract

The invention discloses an algae-inhibiting agent of Ailanthus bark, a preparation method and an application thereof, and belongs to the field of water body pollution control. The algastatic agent is the crude ethanol extract of Ailanthus bark. Compared with the prior art, the present invention overcomes the disadvantages of secondary pollution caused by existing chemical methods, high cost, difficult control, and easy damage to the ecological environment, and the technical cost of extracting allelopathic algae inhibitors from aquatic plants is high and the operation is complicated. limitations. The invention extracts the algae inhibitor from the bark of the woody plant Ailanthus ailanthus, has simple preparation method, convenient use, strong operability and is suitable for large-scale popularization. The algastatic agent prepared by the invention has high inhibitory efficiency to Microcystis aeruginosa, can be effective in the second day, and the 6 inhibitory rate reaches more than 90%, and can effectively protect the water body from algal blooms for a long time.

Description

technical field [0001] The invention belongs to the field of water body pollution control, and in particular relates to an algae-inhibiting agent of Ailanthus bark, its preparation method and application. Background technique [0002] With the development of economy and the increase of human activities, the eutrophication of lakes and reservoirs is becoming more and more serious. Eutrophication of water body leads to frequent outbreaks of cyanobacteria blooms, which not only affects water quality, but also releases harmful toxins, seriously endangering aquatic organisms and even human health. It has become a serious problem facing the current global ecological environment. Therefore, how to effectively control the excessive growth of harmful cyanobacteria, Then preventing algae blooms from happening has become a research focus and frontier in the field of environment. [0003] The current algae control technologies mainly include the following: physical methods such as isol...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C02F1/50
CPCC02F1/50C02F2303/20
Inventor 孟盼盼刘忠德高廷东
Owner TAISHAN UNIV
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