Method for improving laying rate of active immunization of fusion prolactin of a Wulong goose

A technology of active immunization and prolactin, applied in the field of egg production, to achieve the effect of improving nesting and egg production rate, production performance and market value

Inactive Publication Date: 2015-05-27
SHANDONG SINDER TECH
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  • Description
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Problems solved by technology

[0005] Now there are many articles on the effect of recombinant prolactin surface antigen on the nesting and egg production of Wulong geese, the detection of goose prolactin receptor gene PRLRSNPs and its relationship with egg production,

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  • Method for improving laying rate of active immunization of fusion prolactin of a Wulong goose
  • Method for improving laying rate of active immunization of fusion prolactin of a Wulong goose
  • Method for improving laying rate of active immunization of fusion prolactin of a Wulong goose

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Embodiment Construction

[0022] Below in conjunction with embodiment the specific embodiment of the present invention is described in further detail:

[0023] 1. Experimental method:

[0024] (1) cRNA first-strand synthesis and amplification:

[0025] Two Wulong geese were randomly selected on the spot, and the pituitary glands were taken after bloodletting from the neck, and stored in liquid nitrogen at -80°C for later use. Total RNA was extracted from the collected Wulong goose pituitary using TRIzol reagent. Using total RNA as a template, cDNA was synthesized with Easy Script First-Strand cDNA Synthesis Super Mix. 1% agarose gel was used for PCR reaction product detection and electrophoresis, the target gene was detected by electrophoresis, and the gel was cut for recovery.

[0026] (2) Establishment, screening and identification of PRL prokaryotic expression vector:

[0027] The resulting PCR product was digested with double enzymes to obtain the pET32a (+) fragment, which was stored at 4°C fo...

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Abstract

The invention discloses a method for improving laying rate of active immunization of fusion prolactin of a wulong goose. The method comprises the following step: (1) first strand synthesis and amplification of cRNA; (2) building, screening and identifying of a PRL prokaryotic expression vector; (3) inducible expression and crushing of a recombinant PRL protein; (4) SDS-PAGE detection and concentration determination of the recombinant PRL protein; (5) purification and recovery of the recombinant PRL protein; (6) preparation of a recombinant PRL protein injection; and (7) active immunization, observation and recording. The broodiness and the laying rate of the wulong goose can be improved, so that the production performance and the market value of the Wulong goose can be improved.

Description

technical field [0001] The invention relates to the technical field of laying eggs, in particular to a method for actively immunizing Wulong goose with fusion prolactin to improve egg laying rate. Background technique [0002] Wulong goose is an excellent local poultry species in my country. Its outstanding advantages are small size, high egg production and high slaughter rate. At the same time, Wulong goose is also resistant to rough feeding, strong foraging ability, mainly grass-fed, and strong disease resistance. Both grazing and captive breeding can produce eggs seasonally, and their nesting ability is extremely strong, resulting in low egg production. Therefore, eliminating the nesting ability may be an effective means to improve the reproductive performance of Wulong geese. [0003] Nesting performance of poultry is related to changes in plasma PRL levels. The rise of plasma PRL concentration to a certain level can trigger nesting. After the nesting starts, the nestin...

Claims

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Application Information

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IPC IPC(8): A61K39/00C12N15/70C12N15/16C07K14/575C07K1/36C07K1/34C07K1/22
Inventor 李明义刘阳李晓林李彦凤赵航
Owner SHANDONG SINDER TECH
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