Plant leaf shading device and chlorophyll fluorescence determination method applying plant leaf shading device

Abstract

The invention discloses a plant leaf shading device and a chlorophyll fluorescence determination method applying the plant leaf shading device. The shading device comprises a front shading layer which is used for covering the upper surface of a leaf and is provided with an open hole, a rear shading layer which is used for covering the lower surface of the leaf and is connected with the front shading layer, and a movable shading blocking piece which is used for covering the open hole, wherein the front shading layer, the rear shading layer and the movable shading blocking piece are made of a soft light material. The soft light material can be a piece of tin foil paper which is light in weight, low in cost, simple and convenient, can shade effectively, and can be adjusted according to the size of the leaf; the shading area is large; the leaf is not damaged; the plant leaf shading device is suitable for all types of plant leaves. The shading device disclosed by the invention is used for the dark adaptation of the plant leaf in chlorophyll fluorescence determination, is slightly influenced by external environment factors, and is favorable for the stability of data determination; the determination data is relatively close to completely shading conditions.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a plant leaf shader and a method for measuring chlorophyll fluorescence using the shader. Background technique [0002] Chlorophyll fluorescence, as a probe for the study of plant photosynthesis, has not only been widely studied and applied, but also has become an indispensable tool for studying photosynthesis. Almost all changes in the photosynthesis process can be reflected by chlorophyll fluorescence, and the fluorescence measurement technique does not need to break cells and does not harm organisms. Therefore, it is a simple, fast and reliable method to indirectly study the changes in photosynthesis by studying chlorophyll fluorescence. method. [0003] The measurement of chlorophyll fluorescence includes two aspects, namely dark adaptation and light measurement. The maximum fluorescence Fm and the original fluorescence Fo, as well as the maximum quantum yield Fv / Fm=(Fm-Fo) / Fm ...

AI Technical Summary

Problems solved by technology

[0006] An object of the present invention is to provide a shader for plant leaves, which does not damage the leaves, is suitable for all types of plant leaves, is easy to operate and has a good shading effect, thereby overcoming the fact that the shading leaf clips matched with existing instruments are not suitable for small and soft plants The leaves and shading effects are vulnerable to external influences

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