High spatial resolution laser split pupil confocal mass spectrometry imaging method and device

A high spatial resolution microscopic imaging technology, applied in the field of confocal microscopic imaging technology and mass spectrometry imaging, can solve the problems of low spatial resolution of mass spectrometry detection, large laser focus spot, long time of mass spectrometry imaging, etc., to improve the spatial resolution capability , Strong anti-stray light ability, and the effect of overcoming the interference of stray light on the focal plane
CN104697981BActive Publication Date: 2017-03-29BEIJING INSTITUTE OF TECHNOLOGYGY
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Patent Information

Authority / Receiving Office
CN Β· China
Patent Type
Patents(China)
Current Assignee / Owner
BEIJING INSTITUTE OF TECHNOLOGYGY
Publication Date
2017-03-29

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Abstract

The invention relates to a high-spatial resolution laser splitting-pupil confocal mass spectrometry microimaging method and device, and belongs to the technical fields of confocal microimaging and mass spectrometry imaging. According to the method, the splitting-pupil confocal imaging technology and the mass spectrometry and spectrum detection technologies are combined, high-spatial resolution morphological imaging is performed on a sample by use of the focusing micro-spot, treated by use of a super-resolution technology, of a splitting-pupil confocal microscope, a mass spectrometry detection system is used for performing micro-area mass spectrometry imaging on charged molecules, atoms and the like generated by performing desorption ionization on the sample with the focusing spot of a splitting-pupil confocal microscopic system, and then complete sample component information can be obtained by virtue of the fusion and the comparison of the detection data information; as a result, micro-area high-spatial resolution and high-sensitivity imaging and detection on the morphology and the components of the sample to be tested are realized. The method and device are used for overcoming the defect that the existing confocal microimaging technology is incapable of inhibiting the interference of the focal plane stray light, and are high in stray light resisting capability; a brand new effective technical way is provided for high resolution imaging of the biomass spectrometry.
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Description

technical field

[0001] The invention belongs to the field of confocal microscopic imaging technology and mass spectrometry imaging technology, combines split-pupil confocal microscopic imaging technology and mass spectrometry imaging technology, and relates to a high spatial resolution laser split-pupil confocal mass spectrometry microscopic imaging method and device, which can be used for biological mass spectrometry high-resolution imaging.

[0002] technical background

[0003] Mass Spectrometry (Mass Spectrometry) is to ionize the components in the sample, so that the generated charged atoms, molecules or molecular fragments with different charge-to-mass ratios are respectively focused under the action of an electric field and a magnetic field to obtain a sequence of mass-to-charge ratios. Spectrograph instrument. Mass spectrometry imaging is to perform mass spectrometry analysis on multiple small areas in the two-dimensional area of ​​the sample to detect the distributi...

Claims

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