High spatial resolution laser dual-axis confocal mass spectrometry imaging method and device

Abstract

The invention relates to a high-spatial-resolution laser double-axis confocal mass spectrum microimaging method and device, and belongs to the field of cofocal microimaging technology and mass spectrum imaging technology. The method is that the double-axis confocal imaging technology and mass spectrum imaging technology are combined; focusing spots of a high-spatial-resolution double-axis cofocal microscope system are utilized for axial focus fixing and imaging on spicmen; the same focal spots are utilized for desorbing and ionizing a sample to achieve mass spectrum imaging, and then the high-spatial-resolution imaging of micro area image and component of the sample can be achieved. The device comprises a point light source, a collimating lens, a ring light generating system, a measuring object lens, an acquiring lens for detecting strength signals of reflecting light of focusing spots of the measuring object lens, a focusing lens, a strength point detector at a focusing point of the focusing lens, an ionizing sample suction tube for detecting plasma feather component in focusing spot desorption ionization of the measuring object lens, and a mass spectrum detecting system. The method and device can provide a brand new effective technological way for biomass spectrum high-resolution imaging.

Description

technical field [0001] The invention belongs to the field of confocal microscopic imaging technology and mass spectrometry imaging technology, combines biaxial confocal microscopic imaging technology and mass spectroscopic imaging technology, and relates to a high spatial resolution laser biaxial confocal mass spectroscopic microscopic imaging method and device, which can be used for biological mass spectrometry high-resolution imaging. technical background [0002] Mass Spectrometry (Mass Spectrometry) is to ionize the components in the sample, so that the generated charged atoms, molecules or molecular fragments with different charge-to-mass ratios are respectively focused under the action of an electric field and a magnetic field to obtain a sequence of mass-to-charge ratios. Spectrograph instrument. Mass spectrometry imaging is to perform mass spectrometry analysis on multiple small areas in the two-dimensional area of ​​the sample to detect the distribution of substanc...

AI Technical Summary

Problems solved by technology

[0005] 1) Due to the use of simple laser focusing to desorb and ionize the sample, there are still problems such as large laser focusing spot and low spatial resolution of mass spectrometry detection;

[0006] 2) The time required for mass spectrometry imaging is long, and the axial position of the focused spot of the laser mass spectrometer often drifts relative to the sample to be measured

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