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High spatial resolution laser dual-axis differential confocal mass spectrometry imaging method and device

A two-axis differential confocal and high spatial resolution technology, which is applied in the field of confocal microscopy imaging and mass spectrometry imaging, can solve the problems of low spatial resolution of mass spectrometry detection, long mass spectrometry imaging time, and large laser focus spot, etc. Spatial resolution, overcoming the interference of stray light on the focal plane, and strong anti-stray light ability

Active Publication Date: 2018-01-12
BEIJING INSTITUTE OF TECHNOLOGYGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] 1) Due to the use of simple laser focusing to desorb and ionize the sample, there are still problems such as large laser focusing spot and low spatial resolution of mass spectrometry detection;
[0006] 2) The time required for mass spectrometry imaging is long, and the axial position of the focused spot of the laser mass spectrometer often drifts relative to the sample to be measured

Method used

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  • High spatial resolution laser dual-axis differential confocal mass spectrometry imaging method and device
  • High spatial resolution laser dual-axis differential confocal mass spectrometry imaging method and device
  • High spatial resolution laser dual-axis differential confocal mass spectrometry imaging method and device

Examples

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Embodiment 1

[0040] The embodiment of the present invention is based on image 3 The shown high spatial resolution laser dual-axis differential confocal mass spectrometry microscopic imaging device uses a pulsed laser 30, a condensing lens 31 and a light-transmitting optical fiber 33 at the focus of the condensing lens to replace figure 1In the point light source 1, a CCD detector 27 is used to replace the first light intensity point detector 15 and the second light intensity point detector 16 located on the focal plane of the relay magnifying lens 13 and placed symmetrically with respect to the collection optical axis 10 . exist image 3 The exit beam attenuator 34 is introduced into the laser focusing system of the laser, and the detection beam attenuator 35 is introduced into the laser dual-axis differential confocal detection system.

[0041] Such as image 3 As shown, the high spatial resolution laser dual-axis differential confocal mass spectrometer imaging device includes a pulsed...

Embodiment 2

[0061] Such as figure 2 In the shown high spatial resolution laser dual-axis differential confocal mass spectrometer imaging device, the point light source 1 consists of a pulsed laser 30 along the direction of the incident optical axis 7, a condenser lens 31, and a pinhole 32 at the focal point of the condenser lens 31. Instead, the ring light generating system 4 can be replaced by a vector beam generating system 28 and a pupil filter 29, and the first light intensity point detector 15 and the second light intensity point detector 16 located at the focal plane of the relay magnifying lens 13 are controlled by a CCD Detector 27 instead.

[0062] The radially polarized light longitudinal field tight focusing system composed of the vector beam generating system 28, the pupil filter 29 and the measuring objective lens 6 is used to compress the lateral size of the focused spot.

[0063] The remaining imaging measurement methods are the same as in Example 1.

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Abstract

The invention relates to a high-spatial resolution laser biaxial differential confocal mass spectrometry microimaging method and device, and belongs to the technical fields of confocal microimaging and mass spectrometry imaging. According to the method, the biaxial differential confocal imaging technology and the mass spectrometry imaging technology are combined, axial focus fixing and imaging are performed on a sample by use of the focusing spot of a high-spatial resolution biaxial differential confocal system, and the focusing spot of the high-spatial resolution biaxial differential confocal system is used for desorption ionization of the sample to realize mass spectrometry imaging, and consequently, high-spatial resolution imaging of the micro-area images and the components of the sample is realized. The device comprises a point light source, a collimating lens, a ring light generation system and a measuring objective, as well as a collecting lens, a focusing lens, a relay magnifying lens and an intensity detector, all of which are arranged in the direction of the collecting optical axis, and an ionization sample suction pipe for detecting plasma plume components and a mass spectrometry detection system; the inclined angle between an incident optical axis and the collecting optical axis is 2 theta. A brand new effective technical way can be provided for the high resolution imaging of the biomass spectrometry.

Description

technical field [0001] The invention belongs to the field of confocal microscopic imaging technology and mass spectrometry imaging technology, combines biaxial differential confocal microscopic imaging technology and mass spectroscopic imaging technology, and relates to a high spatial resolution laser biaxial differential confocal mass spectroscopic microscopic imaging method and device, It can be used for high-resolution imaging of biological mass spectrometry. technical background [0002] Mass Spectrometry (Mass Spectrometry) is to ionize the components in the sample, so that the generated charged atoms, molecules or molecular fragments with different charge-to-mass ratios are respectively focused under the action of an electric field and a magnetic field to obtain a sequence of mass-to-charge ratios. Spectrograph instrument. Mass spectrometry imaging is to perform mass spectrometry analysis on multiple small areas in the two-dimensional area of ​​the sample to detect th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/64
Inventor 赵维谦邱丽荣
Owner BEIJING INSTITUTE OF TECHNOLOGYGY
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