Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Primer group and kit for detecting human erythrocyte Kell blood type genotyping

A red blood cell and blood type technology, applied in the field of kits for detecting human red blood cell Kell blood type genotyping, can solve the problems of high price and difficult purchase of reagents

Inactive Publication Date: 2015-06-24
天津市秀鹏生物技术开发有限公司
View PDF1 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is: to overcome the high price of serological detection reagents, the deficiencies such as reagents are not easy to buy, provide a kind of immunology method that can simply and conveniently detect the primer set of human erythrocyte Kell blood group gene;

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Primer group and kit for detecting human erythrocyte Kell blood type genotyping
  • Primer group and kit for detecting human erythrocyte Kell blood type genotyping
  • Primer group and kit for detecting human erythrocyte Kell blood type genotyping

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] According to the Kell allele sequence released by the National Center for Biotechnology Information (NCBI Gene Bank), a primer set for detecting the Kell blood group gene of human erythrocytes was designed. The primer set includes K1 primer pair, K2 primer pair and internal reference primers right.

Embodiment 2

[0022] A kit for detecting Kell blood group genes of human erythrocytes, comprising a primer plate coated with a K1 primer pair, a K2 primer pair and an internal reference primer pair and concentrated dNTP-Buffer.

[0023] The preparation method of the test kit for detecting human erythrocyte Kell blood group gene is:

[0024] (1) According to the well location map of human erythrocyte Kell blood group gene-specific primers (see figure 1 ), respectively coat K1 primer pair, K2 primer pair and internal reference primer pair on the corresponding positions of the primer plate, and dry them;

[0025] (2) Prepare 440μl concentrated dNTP-Buffer according to the mixture of 220mM dNTP, 3.5mM Mg2+, 500mM KCL, 100mM Tris-HCL, 1% TritonX-100;

[0026] (3) The primer plate coated with the primer pair includes 2 specific primer wells per person, and the dNTP-Buffer is concentrated to form a kit for detecting the Kell blood group gene of human erythrocytes.

Embodiment 3

[0028] Use operational procedures.

[0029] 1. Preparation of 2.5% agarose gel.

[0030] (1) Add 2.5g agarose to 100ml 1×TBE solution (Tris-borate-EDTA solution), heat until a uniform gel solution is formed. Then add an appropriate amount of electrophoresis dye and mix well.

[0031] (2) Place the gel tank in a horizontal position, add an appropriate amount of gel solution into the gel tank, and insert the electrophoresis comb. Move the gel tank horizontally back and forth to ensure even coverage of the gel solution.

[0032] (3) After the gel is completely solidified, pull out the electrophoresis comb vertically.

[0033] 2. The PCR cycle parameters are shown in Table 1.

[0034] 1 96℃ / 2min 1 cycle 2 96℃ / 20sec, 68℃ / 60sec 5 cycles 3 96°C / 20 sec, 65°C / 45 sec, 72°C / 30 sec 10 cycles 4 96℃ / 20sec, 62℃ / 45sec, 72℃ / 30sec 15 cycles 5 72℃ / 3min 1 cycle 6 Store at 4°C

[0035] 3. The test conditions that must be met.

[0036]...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a primer group and a kit for detecting human erythrocyte Kell blood type genotyping. The primer group for determining a Kell blood type gene comprises a K1 primer pair, a K2 primer pair and an internal reference primer pair. According to the kit disclosed by the invention, restrictions in authentication of Kell blood type by a serum method are overcome; and the kit is indispensable auxiliary means for correct judgment, and has wide application prospect and clinical reference value.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to a kit (PCR-SSP method) for detecting Kell blood type genotyping of human erythrocytes. Background technique [0002] In Europe and America, Kell, ABO, and Rh are listed as the three major blood group systems, which are of great significance in blood transfusion. Both anti-K and anti-K can cause hemolytic transfusion reactions. Although most of the Kell blood group system of Han people in my country is kk, the possibility of producing resistance to K still exists. A survey of 668 cases of Han population in Beijing using anti-K and anti-K blood types found that there were 3 cases of K (+) individuals, and the gene frequency was 0.0022. [0003] The Kell blood group system used serological technology to type. So far, there is no domestic anti-K and -K serum supply. Imported serum is expensive and difficult to popularize. However, the use of DNA typing technology can reduce costs and is...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6876C12Q2600/166
Inventor 赵卫军
Owner 天津市秀鹏生物技术开发有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com